公开(公告)号：KR1020130083233A

公开(公告)日：2013-07-22

申请号：KR1020120003897

申请日：2012-01-12

Applicant: (주)옴니허브

Inventor： 허담 ,  김대익 ,  윤성란

IPC: C12Q1/25 ,  C12Q1/02 ,  G01N33/50 ,  A23F3/10

CPC classification number: G01N31/22 ,  A23F3/10 ,  A23F3/34 ,  G01N21/33 ,  G01N27/447 ,  G01N33/68

Abstract: PURPOSE: A method for verifying an antioxidative effect by fermenting citrus peels and treating with an enzyme is provided to develop and manufacture a high quality drink with an antioxidative function. CONSTITUTION: A method for verifying an antioxdiative effect by fermenting citrus peels and treating with an enzyme comprises the steps of: sterilely inoculating a spawn liquid to autoclaved citrus peels and fermenting; extracting and freeze-drying the citrus peel fermentation; adding the fermentation to the distilled water and extracting in a reflux extractor connected with a cooling device; filtering and concentrating the fermentation; and freeze-drying the fermentation to obtain the solid fermentation powder of the citrus peels. The content of a phenolic compound is measured and the content of flavonoid and the expression of superoxide dismutase are analyzed for testing the antioxidative effect of citrus peels. The antioxidative effect is verified through the test. [Reference numerals] (AA) Dermis; (BB) Enzyme; (CC) Fermentation

Abstract translation: 目的：提供通过发酵柑橘皮和用酶处理来检验抗氧化作用的方法，以开发和制造具有抗氧化功能的高品质饮料。 构成：通过发酵柑橘皮和用酶处理来验证抗氧化作用的方法包括以下步骤：将产卵液无菌接种于高压灭菌的柑橘皮和发酵; 提取和冷冻干燥柑橘皮发酵; 将发酵添加到蒸馏水中并在与冷却装置连接的回流提取器中提取; 过滤和浓缩发酵; 并冷冻干燥发酵，得到柑橘皮的固体发酵粉。 测定酚类化合物的含量，分析黄酮类化合物的含量和超氧化物歧化酶的表达，检测柑橘皮的抗氧化作用。 通过试验验证抗氧化作用。 （标号）（AA）真皮; （BB）酶; （CC）发酵