公开(公告)号：KR101099666B1

公开(公告)日：2011-12-29

申请号：KR1020110063137

申请日：2011-06-28

Applicant: (주)지에프씨

Inventor： 이동걸 ,  강희철 ,  한상근 ,  이찬우

IPC: C12N1/20 ,  C12N9/02 ,  C12N15/53 ,  C12P17/00

CPC classification number: C12N9/0004 ,  C12P17/10 ,  C12R1/01 ,  C12Y114/18001

Abstract: PURPOSE: A method for preparing 5,6-dihydroxy indole using Kitasatospora sp. DG09 is provided to remarkably enhance melanogenesis and tyrosinase production. CONSTITUTION: A method for preparing 5,6-dihydroxy indole comprises: a step of culturing a transformant containing Kitasatospora sp. DG09(deposit number KCCM11178P) or tyrosinase nucleic acid sequence of sequence number 3; and a step of collecting 5,6-dihydroxy indole from the culture liquid. A tyrosinase has an amino acid sequence of sequence number 2 prepared from Kitasatospora sp. DG09. The tyrosinase has a nucleic acid sequence of sequence number 3.

Abstract translation: 目的：使用Kitasatospora sp。制备5,6-二羟基吲哚的方法。 提供DG09显着增强黑素生成和酪氨酸酶的产生。 构成：制备5,6-二羟基吲哚的方法包括：培养含有西葫芦孢菌的转化体的步骤 DG09（保藏号KCCM11178P）或序列号3的酪氨酸酶核酸序列; 以及从培养液中收集5,6-二羟基吲哚的步骤。 酪氨酸酶具有从Kitasatospora sp。制备的序列号2的氨基酸序列。 DG09。 酪氨酸酶具有序列号3的核酸序列。