-
1.发明公开인간 제대혈 유래 내피 전구세포의 배양방법 및 인간 제대혈 유래 내피 전구세포를 포함하는 허혈성 질환의 예방 또는 치료용 조성물 有权用于培养从人体脐带血中衍生的内皮祖细胞的方法和组合物，用于预防或治疗包含人脐带血的内皮祖细胞的化学疾病
公开(公告)号：KR1020140091801A
公开(公告)日：2014-07-23
申请号：KR1020130003204
申请日：2013-01-11
Applicant: (주)차바이오텍
CPC classification number: C12N5/0652 , A61K35/44 , A61K35/51 , A61K38/39
Abstract: The present invention provides a method for culturing endothelial progenitor cells derived from human umbilical cord blood. According to the present invention, the endothelial progenitor cells derived from human umbilical cord blood cultured zare capable of having the cell adhesive ability and proliferation ratio similar to a cell cultured by a conventional cell culturing method. According to the present invention, collagen derived from umbilical cord and umbilical cord extract can be used as serum replacement and adjurvants for culturing a stem cell and a general animal cell. According to the present invention, a vascular endothelial (precursor) cell, and extract and collagen derived from umbilical cord, which are an autologous substance, are capable of preventing an immune rejection response generated when a cell is transplanted; and minimizing the pollution caused by a pollution source derived from an animal by excluding the use of a protein source derived from an animal. The present invention provides a pharmaceutical composition comprising endothelial progenitor cells derived from human umbilical cord blood for preventing or treating ischemic diseases.
Abstract translation: 本发明提供一种培养源自人脐带血的内皮祖细胞的方法。 根据本发明，源自人脐带血培养的能够具有与通过常规细胞培养方法培养的细胞相似的细胞粘附能力和增殖比例的内皮祖细胞。 根据本发明，可以将来自脐带和脐带提取物的胶原蛋白用作血清替代物和用于培养干细胞和一般动物细胞的辅助剂。 根据本发明，作为自体物质的来自脐带的血管内皮（前体）细胞和来源于脐带的提取物和胶原能够防止移植细胞时产生的免疫排斥反应; 并且通过排除使用源自动物的蛋白源来最小化源自动物的污染源造成的污染。 本发明提供一种包含源自人脐带血的内皮祖细胞用于预防或治疗缺血性疾病的药物组合物。
-
公开(公告)号：KR101422689B1
公开(公告)日：2014-07-23
申请号：KR1020120075755
申请日：2012-07-11
Applicant: (주)차바이오텍
IPC: A61K38/39 , A61K31/728 , A61K35/44 , A61K35/12
CPC classification number: A61K38/39 , A61K31/728 , A61K35/51 , A61K35/545 , A61K47/36 , A61L27/26 , A61L27/3834 , A61L27/3852 , A61L27/52 , A61L2400/06 , A61L2430/06 , A61K2300/00 , C08L89/06 , C08L5/08
Abstract: 본 발명은 의료용 복합 생체소재에 관한 것이다. 보다 상세하게는, 본 발명은 콜라겐 및 히알루론산 유도체를 포함하는 의료용 복합 생체소재에 대한 것이다. 또한, 상기 생체소재 및 포유류의 탯줄 유래 줄기세포를 이용한 연골세포치료제에 관한 것이다. 이러한, 생체소재는 면역 반응을 야기하지 않고, 내구성이 우수하며, 이러한 생체소재와 줄기세포를 포함한 연골세포치료제는 관절경을 이용한 시술이 가능하여 환자의 고통을 줄여줄 뿐만 아니라 효과적으로 퇴행성 관절염 및 연골 손상을 치료할 수 있다.
-
公开(公告)号：KR1020130007751A
公开(公告)日：2013-01-21
申请号：KR1020110068261
申请日：2011-07-11
Applicant: (주)차바이오메드 , (주)차바이오텍 , 의료법인 성광의료재단
Abstract: PURPOSE: A method for effectively extracting an active ingredient from umbilical cord is provided to use an umbilical cord extract to a cosmetic composition for dressing the skin and improving skin condition. CONSTITUTION: A method for preparing a mammalian umbilical cord extract comprises: a step of cutting umbilical cord; a step of adding the cut umbilical cord to a buffer solution; a step of stirring the mixture at 4-10 deg.C.; and a step of centrifuging the resultant at 4-10 deg. C. and collecting supernatant. The umbilical cord is cut in a length of 0.5-2 cm. The mammalian includes human, pig, horse, cow, mouse, rat, hamster, rabbit, goat, or sheep. The umbilical cord extract contains 800-2,200 pg/ml of FGF-2(fibroblast growth factor-2), 1,000-2,000 pg/ml of G-CSF(Granulocyte colony stimulating factor), 500-1,500 pg/ml of MCP-1(monocyte chemoattractant protein-1), and 300-2,000 pg/ml of GRO[growth regulated oncogene as a main ingredients.
Abstract translation: 目的：提供一种从脐带中有效提取活性成分的方法，用于将脐带提取物用于化妆品组合物，用于敷料皮肤和改善皮肤状况。 构成：哺乳动物脐带提取物的制备方法，包括：切断脐带的步骤; 将切割的脐带添加到缓冲溶液的步骤; 在4-10℃下搅拌该混合物的步骤。 和在4-10度离心所得物的步骤。 C.收集上清液。 脐带切割成0.5-2厘米的长度。 哺乳动物包括人，猪，马，牛，小鼠，大鼠，仓鼠，兔，山羊或绵羊。 脐带提取物含有800-2,200pg / ml的FGF-2（成纤维细胞生长因子-2），1,000-2,000pg / ml的G-CSF（粒细胞集落刺激因子），500-1,500pg / ml的MCP-1 （单核细胞趋化蛋白-1）和300-2,000pg / ml GRO [生长调节癌基因作为主要成分]。
-
Patent Agency Ranking4.发明授权인간 제대혈 유래 내피 전구세포의 배양방법 및 인간 제대혈 유래 내피 전구세포를 포함하는 허혈성 질환의 예방 또는 치료용 조성물 有权用于培养源自人脐带血和组合物的内皮祖细胞的方法，用于预防或治疗包含人脐带血的内皮祖细胞的缺血性疾病
公开(公告)号：KR101515369B1
公开(公告)日：2015-04-30
申请号：KR1020130003204
申请日：2013-01-11
Applicant: (주)차바이오텍
Abstract: 본발명은인간제대혈유래내피전구세포의배양방법을제공한다. 본발명의방법에의해배양된인간제대혈유래내피전구세포는종래세포배양방법에의해배양된세포와유사한세포부착능및 증식율을나타낸다. 본발명에서탯줄유래콜라겐및 탯줄추출물은일반적인동물세포및 줄기세포배양을위한보조제및 혈청대체물로사용할수 있다. 본발명에서혈관내피 (전구)세포, 탯줄유래콜라겐및 추출물은자가유래물질로서세포이식시 발생할수 있는면역거부반응을예방할수 있으며, 동물유래의단백질원사용을배제함으로써동물유래오염원에의한오염을최소화할수 있다. 본발명은인간제대혈유래내피전구세포를포함하는허혈성질환의예방또는치료용약제학적조성물을제공한다.
-
公开(公告)号：KR101462004B1
公开(公告)日：2014-11-19
申请号：KR1020120075754
申请日：2012-07-11
Applicant: (주)차바이오메드 , 의료법인 성광의료재단 , (주)차바이오텍
CPC classification number: C12N5/0607 , A61K8/985 , A61K35/51 , A61K2800/10 , A61Q19/00 , C12N5/0018 , C12N5/0605 , C12N2502/025 , C12N2509/00
Abstract: 본 발명은 탯줄로부터 유용성분을 효과적으로 추출하는 방법을 제공한다. 본 발명은 유용성분을 포함하는 탯줄 추출물을 제공한다. 본 발명의 탯줄 추출물은 동물 유래의 일반 세포 및 줄기세포 배양을 위한 혈청 대체물로 사용할 수 있다. 또한, 본 발명의 탯줄 추출물은 조직 수복용 필러, 드레싱 및 피부상태 개선용 화장료 조성물에 이용할 수 있다. 또한, 탯줄 추출물을 이용하여 탯줄, 지방 등의 조직 유래 줄기세포 분리 및 배양용 배지 조성물 그리고 이를 이용한 조직 유래 줄기세포 분리 및 배양 방법에 관한 것이다.
-
公开(公告)号：KR1020140008749A
公开(公告)日：2014-01-22
申请号：KR1020120075754
申请日：2012-07-11
Applicant: (주)차바이오메드 , 의료법인 성광의료재단 , (주)차바이오텍
CPC classification number: C12N5/0607 , A61K8/985 , A61K35/51 , A61K2800/10 , A61Q19/00 , C12N5/0018 , C12N5/0605 , C12N2502/025 , C12N2509/00
Abstract: The present invention provides a method for effectively extracting useful components from an umbilical cord. The present invention provides an umbilical cord extract including the useful components. The umbilical cord extract according to the present invention can be used as a serum substitute for cultivating ordinary cells and stem cells derived from an animal. Also, the umbilical cord extract according to the present invention, can be used for a filler for tissue repair, dressing, and improving skin condition. In addition, the present invention relates to a medium composition for isolating and culturing stem cells derived from a tissue, such as an umbilical cord, fatty tissues and the like, and a method for isolating and culturing stem cells derived from the tissue using the same.
Abstract translation: 本发明提供了一种从脐带有效提取有用成分的方法。 本发明提供了包含有用成分的脐带提取物。 根据本发明的脐带提取物可用作培养来自动物的普通细胞和干细胞的血清替代物。 此外，根据本发明的脐带提取物可用于组织修复，敷料和改善皮肤状况的填料。 此外，本发明涉及用于分离和培养源自组织如脐带，脂肪组织等的干细胞的培养基组合物，以及使用该组合物分离和培养源于组织的干细胞的方法 。
-
公开(公告)号：KR1020130009651A
公开(公告)日：2013-01-23
申请号：KR1020120075755
申请日：2012-07-11
Applicant: (주)차바이오텍
IPC: A61K38/39 , A61K31/728 , A61K35/44 , A61K35/12
CPC classification number: A61K38/39 , A61K31/728 , A61K35/51 , A61K35/545 , A61K47/36 , A61L27/26 , A61L27/3834 , A61L27/3852 , A61L27/52 , A61L2400/06 , A61L2430/06 , A61K2300/00 , C08L89/06 , C08L5/08
Abstract: PURPOSE: A medical composite biomaterial containing collagen and hyaluronic acid derivatives is provided to ensure durability and to effectively treat degenerative arthritis and cartilage damage. CONSTITUTION: A composition contains collagen and hyaluronic acid or a derivative thereof. The collagen is type I collagen and is derived from human umbilical cord. The hyalruonic acid derivative is prepared by crosslinking hyaluronic acid using BDDE(1,4-butandiol diglycidyl ether). A medical composite biomaterial contains the composition. A chondrocyte therapeutic agent contains the stem cells, collagen, and hyaluronic acid or the derivative. The stem cells are cultured in a container coated with cell adhesion proteins using a medium for culturing the stem cells. The cell adhesion protein is the collagen, gelatin, fibronectin, laminin, or poly-D-lysine. A composition for an injection for treating damaged cartilage contains the chondrocyte therapeutic agent. A method for treating damaged cartilage comprises a step of administering the composition to a patient.
Abstract translation: 目的：提供含有胶原蛋白和透明质酸衍生物的医用复合生物材料，以确保耐久性，有效治疗退行性关节炎和软骨损伤。 构成：组合物含有胶原蛋白和透明质酸或其衍生物。 胶原蛋白是I型胶原蛋白，来源于人脐带。 通过使用BDDE（1,4-丁二醇二缩水甘油醚）交联透明质酸来制备透明质酸衍生物。 医疗复合生物材料包含组合物。 软骨细胞治疗剂含有干细胞，胶原和透明质酸或其衍生物。 使用培养干细胞的培养基将干细胞培养在用细胞粘附蛋白包被的容器中。 细胞粘附蛋白是胶原，明胶，纤连蛋白，层粘连蛋白或聚-D-赖氨酸。 用于治疗损伤软骨的注射用组合物含有软骨细胞治疗剂。 用于治疗受损软骨的方法包括将组合物给予患者的步骤。
-
8.发明公开포유류의 탯줄유래 줄기세포 분리 또는 배양용 배지 조성물, 그리고 이를 이용한 포유류의 탯줄유래 줄기세포 분리 또는 배양 방법 无效用于分离或培养MAMMALIAN脐带血干细胞的介质，以及分离或培养MAMMALIAN脐带干细胞的方法
公开(公告)号：KR1020130008178A
公开(公告)日：2013-01-22
申请号：KR1020110068761
申请日：2011-07-12
Applicant: (주)차바이오텍 , (주)차바이오메드 , 의료법인 성광의료재단
Abstract: PURPOSE: A medium composition for isolating and culturing mammalian umbilical cord-derived stem cells is provided to quickly obtain a large amount of stem cells with excellent proliferation and differentiation abilities. CONSTITUTION: A medium composition for isolating or culturing mammalian umbilical cord-derived stem cells contains an umbilical cord extract without serum. Mammal includes human, pig, horse, cow, mouse, rat, hamster, rabbit, goat, or sheep. A method for preparing the umbilical cord extract comprises: a step of adding cut umbilical cord to a buffer solution and stirring; and a step of collecting supernatant from the mixed solution. A method for preparing the stem cells from the mammalian umbilical cord comprises: a step of culturing a blood-removed umbilical cord tissue in a container which is coated with a cell adhesion protein and contains the medium composition; and a step of treating the tissue with a medium containing stem cell lysis enzyme and isolating the stem cells. The cell adhesion protein includes mammalian umbilical cord-derived collagen, gelatin, fibronectin, laminin, or poly-D-lysine.
Abstract translation: 目的：提供用于分离和培养哺乳动物脐带血干细胞的培养基组合物，以快速获得大量具有优异增殖和分化能力的干细胞。 构成：用于分离或培养哺乳动物脐带源干细胞的培养基组合物含有不含血清的脐带提取物。 哺乳动物包括人，猪，马，牛，老鼠，大鼠，仓鼠，兔，山羊或绵羊。 制备脐带提取物的方法包括：将切割的脐带加入缓冲溶液并搅拌的步骤; 以及从混合溶液中收集上清液的步骤。 用于从哺乳动物脐带制备干细胞的方法包括：在包被细胞粘附蛋白并容纳培养基组合物的容器中培养除血的脐带组织的步骤; 以及用含有干细胞裂解酶的培养基处理组织并分离干细胞的步骤。 细胞粘附蛋白包括哺乳动物脐带胶原，明胶，纤连蛋白，层粘连蛋白或聚-D-赖氨酸。
-
-
-
-
-
-
-
Search Results
8
records
(took 0.026 seconds)
