-
公开(公告)号：JP3591853B2
公开(公告)日：2004-11-24
申请号：JP22099993
申请日：1993-09-06
Applicant: 信和化工株式会社
Abstract: PURPOSE:To obtain a separating agent capable of efficiently separating enantiomer of an acidic compound and being stable to modification due to an organic solvent by binding an elastase or a partially modified elastase to a carrier. CONSTITUTION:This separating agent is obtained by binding an elastase or an elastase whose molecular structure is partially modified to a carrier. The elastase is obtained by separating acetone extract of swine pancreas with affinity chromatography. A method for binding the elastase to the carrier comprises putting, e.g. an aminopropyl silica gel used as a carrier into a sodium hydrogen carbonate buffer together with a crosslinking agent, stirring the mixture and binding the elastase to the resultant activated aminopropylsilica gel.
-
公开(公告)号：JP3597546B2
公开(公告)日：2004-12-08
申请号：JP22100093
申请日：1993-09-06
Applicant: 信和化工株式会社
Abstract: PURPOSE:To obtain a separating agent capable of separating enantiomer of salbutamol and atenolol and being inexpensive and stable to modification due to an organic solvent by binding a pepsin or a partially modified pepsin to a support. CONSTITUTION:This separating agent is obtained by binding a pepsin or a pepsin whose molecular structure is partially modified to a carrier. The pepsin is preferably obtained by purifying a protease contained in gastric juice of animals by fractionation, etc., using a precipitation absorbent consisting of safranine. Furthermore, a method for binding the pepsin to the carrier comprises e.g. activating an aminopropylsilica gel used as a carrier with a crosslinking agent consisting of N, N-disuccinimidylcarbonate and then binding the compound to the pepsin.
-
Search Results
2
records
(took 0.032 seconds)
