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公开(公告)号：KR101089255B1
公开(公告)日：2011-12-02
申请号：KR1020090049762
申请日：2009-06-05
Applicant: 가톨릭대학교 산학협력단
IPC: C12N11/06 , C12N9/20 , C07K14/195 , C07K14/76
Abstract: 본 발명은 자체적으로 가교제와 반응하여 화학적 가교 응집체를 형성하기 어려운 효소와 같은 표적 단백질에 대해서, 가교제와 화학적으로 반응할 수 있는 가교 보조 단백질을 혼합하여 표적 단백질-가교보조 단백질의 '공동-효소교차응집체(co-Cross-linked enzyme aggregates, co-CLEA)'를 형성할 수 있는 방법과 이와 같이 얻어진 응집체를 이용하여 화학적 변환 반응을 매개할 수 있는 방법을 제안한다. 본 발명에 따라 얻어진 공동-효소교차응집체는 수용성 효소와 비교할 때 온도, pH 및 유기용매에 대한 안정성이 향상됨은 물론이고 반복적인 사용에도 그 활성이 저하되지 않고 유지되므로, 산업적으로 유용하게 적용될 수 있을 것으로 기대된다.
공동-효소교차결합체(co-Cross-Linked Enzyme Aggregates), 아미다아제(Amidase), 로도코커스 에리스로폴리스(Rhodococcus erythropolis), 포토박테리움(Photobacterium lipolyticum), M37 리파아제(M37 Lipase)-
公开(公告)号：KR1020090126680A
公开(公告)日：2009-12-09
申请号：KR1020080052893
申请日：2008-06-05
Applicant: 가톨릭대학교 산학협력단
Abstract: PURPOSE: A method for producing biodiesel by reaction with alcohol and fat is provided to improve productivity and economics without reduction of activity by alcohol. CONSTITUTION: A biodiesel is produced through ester exchange of fat and fatty oil and alcohol under the presence of biological enzyme. The biological enzyme is Photobacterium lipolyticum M37 lipase. A method for producing biodiesel comprises: a step of reacting fat and fatty oil with alcohol and converting to fatty acid-alcohol ester; and a step of isolating the fatty acid-alcohol ester from glycerol ingredient and moisture; The Photobacterium lipolyticum M37 lipase comprises an amino acid sequence of sequence number 1.
Abstract translation: 目的：提供通过与酒精和脂肪反应生产生物柴油的方法，以提高生产力和经济性，而不会降低酒精活性。 构成：在生物酶存在下，通过脂肪和脂肪油和醇的酯交换生产生物柴油。 生物酶是解脂肪解脂M37脂肪酶。 一种生产生物柴油的方法包括：使脂肪和脂肪油与醇反应并转化成脂肪酸 - 醇酯的步骤; 以及从甘油成分和水分中分离脂肪酸 - 醇酯的步骤; 嗜热解脂肪酸M37脂肪酶包含序列号1的氨基酸序列。
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公开(公告)号：KR1020100131075A
公开(公告)日：2010-12-15
申请号：KR1020090049762
申请日：2009-06-05
Applicant: 가톨릭대학교 산학협력단
IPC: C12N11/06 , C12N9/20 , C07K14/195 , C07K14/76
Abstract: PURPOSE: A method for forming co-cross-linked enzyme aggregates(co-CLEA) of target protein-cross linking protein is provided to improve temperature, pH concentration and solubility to organic solvent. CONSTITUTION: A method for forming co-cross-linked enzyme aggregates(co-CLEA) of mixture protein comprises: a step of reacting a target protein with low amino acid content having functional group with crosslinking protein with rich amino acid content having a functional group which is able to form crosslinked protein aggregates. The target protein is Rhodococcus erythropolis-derived amidase of sequence number 1 or Photobacterium lipolyticum-derived M37 lipase of sequence number 3. The crosslinking protein is bovine serum albumin(BSA) of sequence number 5.
Abstract translation: 目的：提供一种形成靶蛋白交联蛋白的共交联酶聚集体（co-CLEA）的方法，以改善温度，pH浓度和对有机溶剂的溶解度。 构成：形成混合蛋白质的共交联的酶聚集体（co-CLEA）的方法包括：使具有官能团的低氨基酸含量的目标蛋白质与富含氨基酸含量的具有官能团的交联蛋白质反应的步骤 其能够形成交联的蛋白质聚集体。 目标蛋白质是序列号为1的红球菌衍生的红酵母衍生物或序列号3的脂解赖氨酸衍生的M37脂肪酶。交联蛋白是序列号5的牛血清白蛋白（BSA）。
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