公开(公告)号：KR101207840B1

公开(公告)日：2012-12-04

申请号：KR1020100013893

申请日：2010-02-16

Applicant: 강원대학교산학협력단

Inventor： 오덕환 ,  김재명 ,  박용배 ,  김중범

IPC: C12N15/11 ,  C12Q1/68

Abstract: 본발명은바실러스세리우스(Bacillus cereus)의구토독소및 설사독소를동시에검출할수 있도록재조합된멀티플렉스중합효소연쇄반응프라이머세트(multiplex polymerase chain reaction primer set), 이를이용한바실러스세리우스의구토독소및 설사독소검출방법및 바실러스세리우스의구토독소및 설사독소검출용검사키트에관한것이다.바실러스세리우스에의한식중독과관련하여종래설사형독소검출법으로는 HPLC, GC, MS 등이이용되었고, 최근중합효소연쇄반응, ELISA 등이개발되어있다. 그러나구토형독소검출방법과관련된연구는전무한실정이다. 이에본 발명은구토형독소를생성하는바실러스세리우스를신속, 정확하게검출할수 있고, 나아가구토독소및 설사독소를동시에검출할수 있도록재조합된멀티플렉스중합효소연쇄반응프라이머세트를제공한다.

公开(公告)号：KR1020110094491A

公开(公告)日：2011-08-24

申请号：KR1020100013893

申请日：2010-02-16

Applicant: 강원대학교산학협력단

Inventor： 오덕환 ,  김재명 ,  박용배 ,  김중범

IPC: C12N15/11 ,  C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q2537/143 ,  C12Q2600/16 ,  C12R1/085

Abstract: PURPOSE: A recombinant multiplex PCR primer for detecting emetic toxin and diarrhetic toxin of Bacillus cereus is provided to accurately and quickly detect both of toxins simultaneously. CONSTITUTION: A multiplex PCR primer is recombined to detect emetic toxin and diarrhetic toxin simultaneously. The multiplex PCR primer is: a forward primer of sequence number 1 and a reverse primer of sequence number 2; a forward primer of sequence number 3 and a reverse primer of sequence number 4; a forward primer of sequence number 5 and a reverse primer of sequence number 6; a forward primer of sequence number 7 and a reverse primer of sequence number 8; a forward primer of sequence number 9 and a reverse primer of sequence number 10; or a forward primer of sequence number 11 and a reverse primer of sequence number 12. A kit for detecting the toxin contains the primer.

Abstract translation: 目的：提供一种用于检测蜡状芽孢杆菌的吐血毒素和腹泻毒素的重组多重PCR引物，用于准确快速地同时检测两种毒素。 构成：多重PCR引物重组以同时检测催吐毒素和腹泻毒素。 多重PCR引物是：序列号1的正向引物和序列号2的反向引物; 序列号3的正向引物和序列号4的反向引物; 序列号5的正向引物和序列号6的反向引物; 序列号7的正向引物和序列号8的反向引物; 序列号9的正向引物和序列号10的反向引物; 或序列号11的正向引物和序列号12的反向引物。用于检测毒素的试剂盒含有引物。