公开(公告)号：KR100844386B1

公开(公告)日：2008-07-07

申请号：KR1020070018222

申请日：2007-02-23

Applicant: 강원대학교산학협력단

Inventor： 이현용 ,  김철희 ,  김효성 ,  권민철 ,  배근중

IPC: C07K14/78 ,  C07K14/00

Abstract: A method for preparing a peptide from cuttlefish collagen is provided to produce the peptide having a low molecular weight by minimizing collagen molecules through cavitation of ultrasonic wave before enzyme treatment, cause no corrosion of a device by chemical materials such as an acid, and increase applicability of the cuttle fish, a conventional waste material, as a material for a cosmetic product because the obtained peptide shows more excellent skin wrinkle inhibitory activity than that of the cuttle fish. A method for preparing a peptide which skin wrinkle inhibitory activity comprises the steps of: (a) treating collagen extracted from cuttlefish at a temperature of 20 deg.C with ultrasonic wave of 40KHz for 30 minutes using an ultrasonic extractor; and (b) adding 0.5 wt.% of collagenase to a product obtained from the step(a) and hydrolyzing it for 1 hour at room temperature.

Abstract translation: 提供从墨鱼胶原蛋白制备肽的方法，通过在酶处理之前通过超声波的空化使胶原分子最小化，使化学物质如酸不会腐蚀装置，从而提高低分子量的肽，并且增加适用性 作为化妆品的材料，作为常规的废弃物的鱼，因为所获得的肽显示比鱼汁更好的皮肤皱纹抑制活性。 一种制备皮肤皱纹抑制活性肽的方法，包括以下步骤：（a）使用超声波提取器，在20℃的超声波40KHz的超声波处理20分钟的墨鱼中提取的胶原蛋白30分钟; 和（b）将0.5重量％的胶原酶加入到从步骤（a）获得的产物中，并在室温下水解1小时。

公开(公告)号：KR100808115B1

公开(公告)日：2008-02-29

申请号：KR1020060119102

申请日：2006-11-29

Applicant: 강원대학교산학협력단

Inventor： 이현용 ,  김철희 ,  김효성 ,  권민철 ,  송영규 ,  배근중

IPC: C12N1/12

Abstract: A method for preparing a spirulina medium is provided to be able to improve the spirulina medium by using purified deep sea water through precipitation and filtration, thereby the prepared medium capable of promoting the growth of the spirulina and efficiently mass-producing the same. A method for preparing a spirulina medium comprises the steps of: (a) preparing a culture medium by putting 18.61 grams of sodium hydrogen carbonate in 500ml of distilled water without adding sodium carbonate and potassium dihydrogen diphosphate thereto; and (b) mixing 10% of deep sea water, which is obtained by being precipitated at a temperature of 40 deg.C for 7 days and then filtered through 0.2mum filter, with the culture medium.

Abstract translation: 提供螺旋藻培养基的制备方法，能够通过沉淀和过滤使用纯化的深海水来改善螺旋藻培养基，从而能够促进螺旋藻的生长并有效地大量生产螺旋藻培养基。 制备螺旋藻培养基的方法包括以下步骤：（a）通过将18.61克碳酸氢钠放入500ml蒸馏水中而不加入碳酸钠和二氢磷酸二氢钾，制备培养基; 和（b）将通过在40℃的温度下沉淀得到的10％深海水混合7天，然后用培养基通过0.2mum过滤器过滤。