公开(公告)号：KR1020090057760A

公开(公告)日：2009-06-08

申请号：KR1020070124481

申请日：2007-12-03

Applicant: 재단법인서울대학교산학협력재단 ,  강원대학교산학협력단

Inventor： 서진호 ,  안지현 ,  김명동 ,  조제원

IPC: C12Q1/68 ,  C12N15/11

CPC classification number: C12Q2545/107

Abstract: A primer kit for detecting Lactobacillus brevis is provided to accurately monitor the Lactobacillus brevis from a fermentation material such as Kimchi. A primer kit for detecting a Lactobacillus brevis comprises: a primer set which contains a forward primer of the sequence number 5 and reverse primer of the sequence number 6 and amplifies a part of sequence in 16S rRNA gene of Lactobacillus brevis; a primer set which contains a forward primer of the sequence number 3 and reverse primer of the sequence number 4 and amplifies a part of sequence in dextransucrase gene; and a primer set which contains a forward primer of the sequence number 1 and reverse primer of the sequence number 2.

Abstract translation: 提供了一种用于检测短乳杆菌的引物试剂盒，用于从泡菜等发酵材料中准确地监测短乳杆菌（Lactobacillus brevis）。 用于检测短乳杆菌的引物试剂盒包括：引物组，其含有序列号5的正向引物和序列号6的反向引物，并扩增短乳杆菌16S rRNA基因中的一部分序列; 包含序列号3的正向引物和序列号4的反向引物的引物组，并扩增葡聚糖蔗糖酶基因中的一部分序列; 以及包含序列号1的正向引物和序列号2的反向引物的引物组。

公开(公告)号：KR1020110093135A

公开(公告)日：2011-08-18

申请号：KR1020100012986

申请日：2010-02-11

Applicant: 강원대학교산학협력단

Inventor： 서진호 ,  김명동 ,  이원흥 ,  이태희 ,  장미희 ,  유종길

IPC: C12N1/21 ,  C12N15/31 ,  C12N15/53 ,  C12R1/865

Abstract: PURPOSE: A method for producing metabolic products is provided to supply enough NADPH and to enhance production of the metabolic products. CONSTITUTION: A method for producing metabolic products comprises step of transforming Saccharomyces cerevisiae-derived POS5 gene to E.coli by singular or plural enzyme reactions. A mitochondria-targeting sequence is deleted in POS5 gene. Glucose-6-phosphate dehydrogenase(G6PDH) is overexpressed in the E.coli.

Abstract translation: 目的：提供生产代谢产物的方法以提供足够的NADPH并增强代谢产物的产生。 构成：生产代谢产物的方法包括通过单数或多次酶反应将酿酒酵母衍生的POS5基因转化到大肠杆菌的步骤。 线粒体靶向序列在POS5基因中被删除。 葡萄糖-6-磷酸脱氢酶（G6PDH）在大肠杆菌中过表达。

公开(公告)号：KR101151429B1

公开(公告)日：2012-06-01

申请号：KR1020100012986

申请日：2010-02-11

Applicant: 강원대학교산학협력단

Inventor： 서진호 ,  김명동 ,  이원흥 ,  이태희 ,  장미희 ,  유종길

IPC: C12N1/21 ,  C12N15/31 ,  C12N15/53 ,  C12R1/865

Abstract: 본발명은 NADPH를보효소로필요로하는효소반응이최종대사산물의합성경로상에단수또는복수로구비된대사산물의생산방법에있어서, 사카로마이세스세레비지애() 유래의유전자 (미토콘드리아에존재하는 NADH 키나제(kinase) 단백질을암호화하는유전자)로형질전환된대장균을이용하는것을특징으로하는데, 세포내에상대적으로높은농도로존재하는 NADH를 NADPH로직접전환함으로써대조군보다 NADPH를충분히공급할수 있으므로, 생합성과정에 NADPH를필요로하는대사산물의생산량을향상시킬수 있는효과가발휘된다.