Abstract:
The present invention relates to a single-stranded nucleic acid aptamer selectively binding to Escherichia coli and a method for detecting Escherichia coli using the same. The method, a kit, or a sensor of the present invention can specifically detect Escherichia coli among microorganisms in water, and can be applied to food hygiene or medical diagnosis.
Abstract:
여러 종류의 미생물에 공통적이고 선택적으로 결합하는 단일가닥 핵산 앱타머 및 상기 앱타머의 제조방법을 제공한다. 본 발명의 일 양상에 따르면 다양한 미생물들을 동시에 검출하거나 진단할 수 있는 프로브를 제공할 수 있으며, 또한 이러한 기능을 갖는 앱타머를 제조하는 방법을 제공할 수 있다.
Abstract:
PURPOSE: A bioreceptor-immobilized titanium dioxide is provided to generate oxidative radicals through crosslinking between functional groups conjugated to the bioreceptor and to show effective antibacterial properties. CONSTITUTION: A titanium dioxide particle has a bioreceptor which specifically binds to microorganisms through a bond between a functional group conjugated to the titanium dioxide particles and a functional group of a receptor. A selective antibacterial method according to the kinds of antibodies immobilized on the titanium dioxide particles using antibody-immobilized titanium dioxide particles comprises the steps of: making a titanium dioxide-antibody complex come in contact with microorganisms using the antibody-immobilized titanium dioxide particles; and irradiating the titanium dioxide-antibody complex by UV rays.
Abstract:
본발명은 DNA 앱타머의스크리닝방법에대한것으로, 더욱상세하게는랜덤핵산라이브러리와대상으로하는표적물질을혼합하여결합을유도하는표적물질반응단계와상기표적물질반응단계후에특정분리방법을실시하여표적물질과결합하지않은핵산을제거하고표적물질과핵산이결합하여형성된핵산결합체를회수하는결합체회수단계와약하게결합한핵산을제거하기위해핵산결합체에대해특정분리방법을실시하여표적물질과결합하지않은핵산을제거하여핵산결합체를재회수하는재회수단계와상기재회수단계가복수번 반복실시된후에최종적으로재회수된핵산결합체에서핵산을분리하는핵산분리단계를포함하여, 표적물질에특이적으로결합하는 DNA 앱타머를간단하고빠르게발굴할수 있는 DNA 앱타머스크리닝방법에대한것이다.