公开(公告)号：KR100840144B1

公开(公告)日：2008-06-23

申请号：KR1020070048230

申请日：2007-05-17

Applicant: 경상대학교산학협력단

Inventor： 윤태진 ,  이증훈 ,  김창덕 ,  최태영 ,  김진화

IPC: C12Q1/00 ,  C12Q1/68

CPC classification number: G01N33/5008 ,  C12N15/64

Abstract: A method for screening a material with whitening activity is provided to use an NQ01(NADPH quinone reductase 1) involving with an in vivo pigmentation and having an amino acid sequence of SEQ ID : NO. 1, 2 or 3 or an isoform thereof as a target for screening the material with whitening activity. A method for screening a material with whitening activity comprises the steps of: (a) treating a protein having an amino acid sequence selected from the group consisting of amino acid sequences of SEQ ID : NOs. 1 to 3 with a candidate material; and (b) selecting a material decreasing the activity of the protein by measuring activity change of the protein obtained form the step(a) through a benzo(a)pyrene quinones-reduction analysis or Western blotting.

Abstract translation: 提供用于筛选具有增白活性的材料的方法，以使用涉及体内色素沉着并具有SEQ ID NO：1的氨基酸序列的NQ01（NADPH醌还原酶1）。 1,2或3或其同种型作为筛选具有增白活性的材料的靶标。 用于筛选具有增白活性的材料的方法包括以下步骤：（a）处理具有选自SEQ ID：NO的氨基酸序列的氨基酸序列的蛋白质。 1至3名候选材料; （b）通过苯并（a）芘醌还原分析或Western印迹法测定步骤（a）获得的蛋白质的活性变化来选择降低蛋白质活性的物质。