콩 단백질 가수분해물을 포함하는 배양 배지를 이용하여 인간 각질형성세포의 체외 증식을 강화시키고 분화를 지연시키는 배양 방법
    2.
    发明公开
    콩 단백질 가수분해물을 포함하는 배양 배지를 이용하여 인간 각질형성세포의 체외 증식을 강화시키고 분화를 지연시키는 배양 방법 无效
    使用含有大量蛋白质水解酶的培养基刺激细胞增殖和人类杀伤细胞的抑制分化的制备方法

    公开(公告)号:KR1020100024305A

    公开(公告)日:2010-03-05

    申请号:KR1020080083100

    申请日:2008-08-25

    Abstract: PURPOSE: A method for culturing human keratinocytes is provided to strengthen in vitro proliferation and delay differentiation with less risk. CONSTITUTION: A medium for culturing human keratinocytes in vitro contains 24ug/ml of bovine pituitary extract and 0.25-2 g/L of soybean protein hydrolysate A method for culturing the human keratinocytes comprises: a step of isolating keratinocyte from preputium from a 10-15 year old child by maintaining a skin tissue in 0.05% trypsin-0.53mM EDTA solution at 4°C for 16 hours; a step of collecting keratinocytes by shaking the skin tissue vigorously; and a step of culturing the keratinocytes in a medium containing 0.2 ng/ml of EGF(Epidermal Growth Factor), 5ug/ml of insulin, 5ug/ml of transferring, 0.18 ug/ml of hydrocortisone, 0.2%(v/v) of bovine pituitary extract(BPE).

    Abstract translation: 目的:提供培养人角质形成细胞的方法,以加强体外增殖和延缓分化,降低风险。 构成:体外培养人角质形成细胞的培养基含有24ug / ml的牛垂体提取物和0.25-2g / L的大豆蛋白水解物。培养人角质形成细胞的方法包括:从10-15分离角质形成细胞与前体的步骤 在0.05%胰蛋白酶0.53mM EDTA溶液中于4℃维持皮肤组织16小时; 通过大力摇动皮肤组织收集角质形成细胞的步骤; 在含有0.2ng / ml EGF(表皮生长因子),5ug / ml胰岛素,5ug / ml转移量,0.18μg/ ml氢化可的松,0.2%(v / v)的血小板浓度的培养基中培养角化细胞的步骤 牛垂体提取物(BPE)。

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