Abstract:
본 발명은 사용환경에서 자연적으로 분해되는 분해성 초음파 변환자에 관한 것으로, 본 발명의 실시예에 따른 분해성 초음파 변환자는 분해성 압전소자를 포함하는 압전층(10), 압전층(10)의 일측에 배치되는 분해성 제1 전극(20), 및 압전층(10)의 타측에 배치되는 분해성 제2 전극(30)을 포함한다.
Abstract:
PURPOSE: A method for culturing human keratinocytes is provided to strengthen in vitro proliferation and delay differentiation with less risk. CONSTITUTION: A medium for culturing human keratinocytes in vitro contains 24ug/ml of bovine pituitary extract and 0.25-2 g/L of soybean protein hydrolysate A method for culturing the human keratinocytes comprises: a step of isolating keratinocyte from preputium from a 10-15 year old child by maintaining a skin tissue in 0.05% trypsin-0.53mM EDTA solution at 4°C for 16 hours; a step of collecting keratinocytes by shaking the skin tissue vigorously; and a step of culturing the keratinocytes in a medium containing 0.2 ng/ml of EGF(Epidermal Growth Factor), 5ug/ml of insulin, 5ug/ml of transferring, 0.18 ug/ml of hydrocortisone, 0.2%(v/v) of bovine pituitary extract(BPE).