公开(公告)号：KR102205558B1

公开(公告)日：2021-01-20

申请号：KR1020190147011

申请日：2019-11-15

Applicant: 고려대학교 산학협력단

Inventor： 정남현 ,  박우준 ,  이종구 ,  한상현 ,  장인동

IPC: C12N11/14 ,  C04B40/06 ,  C04B103/00

Abstract: 본발명은콘크리트자기치유성능을향상시킬수 있는미생물고정을위한코팅담체및 이의제조방법에관한것으로, 구체적으로본 발명은자기치유성능이향상된콘크리트담체의제조방법, 상기방법으로제조된자기치유성능이향상된콘크리트담체, 자기치유성능이향상된콘크리트담체를포함하는시멘트또는콘크리트용조성물및 상기조성물을시멘트또는콘크리트구조물의균열부위에도포하는단계를포함하는시멘트또는콘크리트구조물의균열보수방법에관한것이다. 본발명에따른미생물고정을위한코팅담체를사용할경우, 탄산칼슘석출미생물을담체내에서효율적으로보호할수 있고콘크리트내부에서의생존율을증가시킬수 있어친환경적으로콘크리트의균열을보수할수 있고내구성을증진시킬수 있어콘크리트의자기치유능력을향상시킬수 있다. 따라서본 발명에따른코팅된담체는친환경적인자기치유콘크리트의골재로써활용가능하고기존의시멘트및 폴리머수지표면처리기법의단기적인치유기술을대체할수 있는보다효과적이며지속가능한기술로활용될수 있다.

公开(公告)号：KR1020100024305A

公开(公告)日：2010-03-05

申请号：KR1020080083100

申请日：2008-08-25

Applicant: 고려대학교 산학협력단

Inventor： 정남현 ,  이용권 ,  김승열 ,  김기헌 ,  전복환

IPC: C12N5/071 ,  C12N5/04 ,  C12N5/00

Abstract: PURPOSE: A method for culturing human keratinocytes is provided to strengthen in vitro proliferation and delay differentiation with less risk. CONSTITUTION: A medium for culturing human keratinocytes in vitro contains 24ug/ml of bovine pituitary extract and 0.25-2 g/L of soybean protein hydrolysate A method for culturing the human keratinocytes comprises: a step of isolating keratinocyte from preputium from a 10-15 year old child by maintaining a skin tissue in 0.05% trypsin-0.53mM EDTA solution at 4°C for 16 hours; a step of collecting keratinocytes by shaking the skin tissue vigorously; and a step of culturing the keratinocytes in a medium containing 0.2 ng/ml of EGF(Epidermal Growth Factor), 5ug/ml of insulin, 5ug/ml of transferring, 0.18 ug/ml of hydrocortisone, 0.2%(v/v) of bovine pituitary extract(BPE).

Abstract translation: 目的：提供培养人角质形成细胞的方法，以加强体外增殖和延缓分化，降低风险。 构成：体外培养人角质形成细胞的培养基含有24ug / ml的牛垂体提取物和0.25-2g / L的大豆蛋白水解物。培养人角质形成细胞的方法包括：从10-15分离角质形成细胞与前体的步骤 在0.05％胰蛋白酶0.53mM EDTA溶液中于4℃维持皮肤组织16小时; 通过大力摇动皮肤组织收集角质形成细胞的步骤; 在含有0.2ng / ml EGF（表皮生长因子），5ug / ml胰岛素，5ug / ml转移量，0.18μg/ ml氢化可的松，0.2％（v / v）的血小板浓度的培养基中培养角化细胞的步骤 牛垂体提取物（BPE）。