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公开(公告)号：KR1020120041823A
公开(公告)日：2012-05-03
申请号：KR1020100083537
申请日：2010-08-27
Applicant: 고려대학교 산학협력단
CPC classification number: G01N33/6896 , C12Q1/6844 , C12Q2600/158 , G01N33/15 , G01N33/5302
Abstract: PURPOSE: A proteinic marker for the early diagnosis of Alzheimer's disease is provided to effectively diagnose neurodegenerative diseases. CONSTITUTION: A proteinic marker for the early diagnosis of Alzheimer's disease includes: ATP synthase subunit beta of 55-57 kD containing an amino acid sequence of sequence number 1; adenosine kinase(Isoform Long) of 39-41 kD containing an amino acid sequence of sequence number 2; or regucalcin of 32-34 kD containing an amino acid of sequence number 3. A composition for the early diagnosis Alzheimer's disease contains a material for detecting the presence, amount, or pattern of the proteinic marker.
Abstract translation: 目的：提供早期诊断阿尔茨海默病的蛋白质标志物，以有效诊断神经退行性疾病。 构成：用于早期诊断阿尔茨海默病的蛋白质标记包括：含有序列1的氨基酸序列的55-57kD的ATP合酶亚基β; 含有序列号2的氨基酸序列的39-41kD的腺苷激酶（Isoform Long） 或含有序列号3的氨基酸的32-34kD的聚蛋白。用于早期诊断阿尔茨海默病的组合物含有用于检测蛋白质标记物的存在，量或模式的材料。
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公开(公告)号：KR1020120041821A
公开(公告)日：2012-05-03
申请号：KR1020100083508
申请日：2010-08-27
Applicant: 고려대학교 산학협력단
CPC classification number: G01N33/6896 , C12Q1/6844 , C12Q2600/158 , G01N33/15
Abstract: PURPOSE: A proteinic marker for diagnosing Alzheimer's disease and a composition containing a material for detecting marker change are provided to diagnose and treat Alzheimer's disease. CONSTITUTION: A proteinic marker for diagnosing Alzheimer's disease includes: secernin-1 of 45-47 kD, containing an amino acid sequence of sequence number 1; V-type proton ATPase subunit E1(Atp6v1e1) of 25-27 kD, containing an amino acid sequence of sequence number 2; carbonic anhydrase II of 28-30 kD, containing an amino acid sequence of sequence number 3; glutathione S-transferase of 23-25 kD, containing an amino acid sequence of sequence number 4. A composition for diagnosing Alzheimer's disease contains a material for detecting the presence, amount, or pattern of the proteinic marker.
Abstract translation: 目的：用于诊断阿尔茨海默氏病的蛋白质标记物和含有检测标志物变化的物质的组合物用于诊断和治疗阿尔茨海默氏病。 构成：用于诊断阿尔茨海默病的蛋白质标志物包括：含有序列1的氨基酸序列的45-47kD的Secernin-1; 25-27kD的V型质子ATP酶亚基E1（Atp6v1e1）含有序列号2的氨基酸序列; 碳酸酐酶II为28-30kD，含有序列号3的氨基酸序列; 23-25kD的谷胱甘肽S-转移酶，含有序列号4的氨基酸序列。用于诊断阿尔茨海默病的组合物含有用于检测蛋白质标记物的存在，量或模式的材料。
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公开(公告)号：KR101240207B1
公开(公告)日：2013-03-06
申请号：KR1020100083537
申请日：2010-08-27
Applicant: 고려대학교 산학협력단
Abstract: 본 발명은 알츠하이머병 조기진단용 단백질성 마커, 상기 단백질성 마커의 변화를 검출하는 물질을 포함하는 알츠하이머병 조기진단용 조성물, 상기 알츠하이머병 조기진단용 조성물을 포함하는 알츠하이머병 조기진단용 키트, 상기 알츠하이머병 조기진단용 단백질성 마커를 검출하는 방법, 및 상기 단백질성 마커를 활용한 알츠하이머병 치료제의 스크리닝 방법에 관한 것이다.
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公开(公告)号：KR100863466B1
公开(公告)日：2008-10-16
申请号：KR1020070080131
申请日：2007-08-09
Applicant: 고려대학교 산학협력단
CPC classification number: C07K1/145 , C12N5/0006
Abstract: A method for purification of plasma proteins from an animal cell is provided to remove a large quantity of fat and sugar from the plasma proteins simultaneously through biotinylation, improve yield and purity of plasma proteins by only two-stage purification, and reduce the purification time and costs. A method for purification of plasma proteins from an animal cell comprises the steps of: pulverizing the animal cell by a homogenizer, and centrifuging the pulverized cell to precipitate the nucleus; remove the nucleus, and ultracentrifuging the supernatant with 25-35%(w/v) percoll; and extracting a crude plasma membrane(PM) formed by percoll, and extracting the plasma membrane through two-phase extraction method of 6-7%(w/v) polyethylene glycol and 6-7%(w/v) dextran.
Abstract translation: 提供用于从动物细胞中纯化血浆蛋白的方法，通过生物素化同时从血浆蛋白中除去大量的脂肪和糖，仅通过两步纯化提高血浆蛋白的产量和纯度，并减少纯化时间和 成本。 用于从动物细胞中纯化血浆蛋白的方法包括以下步骤：通过均化器粉碎动物细胞，并将粉碎的细胞离心沉淀细胞核; 去除细胞核，并用25-35％（w / v）percoll将上清液超速离心; 提取通过percoll形成的粗质质膜（PM），并通过6-7％（w / v）聚乙二醇和6-7％（w / v）葡聚糖的两相萃取方法提取质膜。
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