公开(公告)号：KR100729147B1

公开(公告)日：2007-06-19

申请号：KR1020060056350

申请日：2006-06-22

Applicant: 고려대학교 산학협력단

Inventor： 홍석인 ,  송민정 ,  윤동화

IPC: G01N33/53 ,  G01N33/573

Abstract: A high sensitive bio-sensor and a complex bio-sensor therewith are provided to simultaneously detect various markers for liver function including cholesterol, bilirubin, alanine aminotransferase and aspartate transferase, and minimize the size of the bio-sensor by using a thin film process. The high sensitive bio-sensor comprises: a relative electrode terminal portion(124) accumulated on the upper portion of a silicon substrate(130), and electronically connected to an outer electric source; a relative electrode portion(120) located in the end of a relative electrode wiring portion(122) extended from the relative electrode terminal portion in one direction; an operation electrode portion(106) comprising a plurality of irregularities accumulated on the upper portion of the silicon substrate oppositely to the relative electrode portion; an enzyme-immobilizing portion(100) fixed to the upper portion of the operation electrode portion via covalent bond and comprising enzymes capable of reacting with a certain substrate; an operation electrode wiring portion(102) extended from the operation electrode wiring portion in one direction and located in parallel to the relative electrode wiring portion; an operation electrode terminal portion(104) connected to the outer electrical circuit located to the end of the relative electrode wiring portion; and a reference electrode terminal portion(114) accumulated between the relative electrode wiring portion and operation electrode wiring portion, and located to the end of a reference electrode wiring portion(112) extended from the operation electrode terminal portion to the relative electrode terminal portion in parallel, wherein the operation electrode portion contains a platinum thin film.

Abstract translation: 提供高灵敏度生物传感器和复杂生物传感器以同时检测肝脏功能的各种标志物，包括胆固醇，胆红素，丙氨酸转氨酶和天冬氨酸转移酶，并通过使用薄膜工艺使生物传感器的尺寸最小化。 所述高灵敏度生物传感器包括：相对电极端子部分（124），所述相对电极端子部分（124）积聚在硅衬底（130）的上部上并电连接到外部电源; 相对电极部分（120），位于从相关电极端子部分向一个方向延伸的相对电极配线部分（122）的端部; 操作电极部分（106），其包括与所述相对电极部分相对地累积在所述硅基板的上部上的多个不规则部分; 通过共价键固定到操作电极部分的上部的酶固定部分（100），并且包含能够与特定底物反应的酶; 操作电极布线部分，从操作电极布线部分沿一个方向延伸并平行于相关电极布线部分; 连接到位于相对电极布线部分的端部的外部电路的操作电极端子部分（104） 和参考电极端子部分（114），积聚在相关电极布线部分和操作电极布线部分之间，并且位于从操作电极端子部分延伸到相对电极端子部分的参考电极布线部分（112）的端部 其中操作电极部分包含铂薄膜。