公开(公告)号：KR100836669B1

公开(公告)日：2008-06-10

申请号：KR1020070002774

申请日：2007-01-10

Applicant: 고려대학교 산학협력단

Inventor： 구만복 ,  박경서 ,  김병찬 ,  윤철희 ,  김한나

IPC: C12Q1/68

CPC classification number: C12Q1/6837

Abstract: A Japanese medaka DNA chip is provided to be used for evaluating toxicity of an environment hormone or an environmental stress inducing material and detecting the hormone or the inducing material. A DNA chip for evaluating toxicity of an environmental hormone consists of Japanese medaka cDNA having sequences of SEQ ID : NOs. 1 to 574. A method for preparing the DNA chip comprises the steps of: (a) synthesizing cDNA from mRNA extracted from a liver tissue of the Japanese medaka; (b) inserting the cDNA into a plasmid to construct a cDNA library; (c) sequencing the cDNA library to construct a local database including genetic information; (d) classifying the local database using a local BLAST function in a BIOEDIT(NCBI) program into cDNA groups, each of which is not overlapped with one another; and (e) spotting only representative cDNAs from the classified cDNA groups on a chip. Further, the environmental hormone is selected from a group consisting of 17beta-estradiol(E2), bisphenol A(BPA), 2-chlorophenol, Phenol and nonyl phenol.

Abstract translation: 提供日本medaka DNA芯片，用于评估环境激素或环境应激诱导材料的毒性并检测激素或诱导材料。 用于评价环境激素的毒性的DNA芯片由具有SEQ ID NO：1的序列的日本medaka cDNA组成。 一种制备DNA芯片的方法包括以下步骤：（a）从日本medaka的肝组织提取的mRNA合成cDNA; （b）将cDNA插入质粒构建cDNA文库; （c）对cDNA文库进行测序以构建包括遗传信息在内的本地数据库; （d）使用BIOEDIT（NCBI）程序中的局部BLAST功能将本地数据库分类为cDNA组，每组不相互重叠; 和（e）从芯片上分类的cDNA组中仅鉴别出代表性的cDNA。 此外，环境激素选自17β-雌二醇（E2），双酚A（BPA），2-氯苯酚，苯酚和壬基苯酚。