公开(公告)号：KR100839185B1

公开(公告)日：2008-06-17

申请号：KR1020070034732

申请日：2007-04-09

Applicant: 고려대학교 산학협력단

Inventor： 이광원 ,  이현순 ,  홍충의 ,  정성훈 ,  김종탁

IPC: A61K31/7028 ,  A61K36/68 ,  A61P3/10

CPC classification number: A61K31/7028 ,  A23L33/105 ,  A61K31/05 ,  A61K31/235 ,  A61K36/68 ,  A61K2236/33 ,  B01D15/34 ,  Y10S514/866

Abstract: A composition comprising plantamajoside derived from a natural plant is provided to show increased efficacy than a conventional diabetes/diabetic complications inhibiting material without side effects and toxicity, thereby being widely used as a health functional food or a medicine for preventing and treating the diabetes/diabetic complications. A composition for preventing or treating diabetes and diabetic complications comprises plantamajoside derived from Plantago asiatica as an effective ingredient. A method for preparing the composition comprises the steps of: (a) powderizing an alcohol extract of the Plantago asiatica; (b) after dissolving the powder in water, extracting the solution in sequence using hexane, chloroform, ethyl acetate and butanol in accordance with a polarity; and (c) subjecting the organic solvent extract obtained from the step(b) to gel filtration chromatography. Further, the gel filtration chromatography is performed by using a silica gel 60 or Sepadex LH-20.

Abstract translation: 提供了包含来源于天然植物的植物苷的组合物，以显示比常规的糖尿病/糖尿病并发症抑制物质没有副作用和毒性增加的功效，从而被广泛用作健康功能食品或用于预防和治疗糖尿病/糖尿病的药物 并发症。 用于预防或治疗糖尿病和糖尿病并发症的组合物包括源自车前草的植物苷作为有效成分。 制备组合物的方法包括以下步骤：（a）粉化车前草的醇提取物; （b）将粉末溶解在水中后，依次使用己烷，氯仿，乙酸乙酯和丁醇依次萃取溶液; 和（c）使由步骤（b）获得的有机溶剂提取物进行凝胶过滤层析。 此外，通过使用硅胶60或Sepadex LH-20进行凝胶过滤层析。

公开(公告)号：KR100741350B1

公开(公告)日：2007-07-20

申请号：KR1020060002721

申请日：2006-01-10

Applicant: 고려대학교 산학협력단

Inventor： 이광원 ,  이현순 ,  홍충의 ,  정성훈

IPC: A61K36/68 ,  A61P17/18 ,  A61P35/00

Abstract: 본 발명은 식물체 분말을 추출용매인 에탄올에 녹인 후, 끓는 점에서 2-4시간 동안 환류 냉각 추출하고, 얻어진 추출물을 2,000-3,000rpm으로 7-15분 동안 원심분리한 후 상등액만을 여과하여 조 추출물(crude extract)을 제조하는 단계; 상기 조 추출물을 건조시켜 추출용매를 완전히 제거한 후, 물에 용해시키는 단계; 및 상기 물에 용해된 조 추출물에 대하여 이온교환수지에서 에탄올 0%-100%의 농도 구배로 용출하여 단계별 획분을 얻는 단계를 포함하는 식물 유효성분의 추출정제방법을 제공한다.
본 발명에 의하는 경우, 기존 식물 추출물의 추출과 정제 방법을 단회로 단순화함으로써, 추출용매와 칼럼 레진의 사용을 줄일 수 있을 뿐만 아니라, 유기용매 추출과정 또한 단축할 수 있어 시간 및 비용의 효율성을 높일 수 있게 된다.
식물추출물, 정제방법, 차전초, 플랜타마조사이드, 에탄올

公开(公告)号：KR1020080092664A

公开(公告)日：2008-10-16

申请号：KR1020070036287

申请日：2007-04-13

Applicant: 고려대학교 산학협력단

Inventor： 이광원 ,  이현순

IPC: A61K36/185 ,  A61P3/10

Abstract: A composition comprising chebulic acid is provided to inhibit production of AGEs(advanced glycation end-products) and remove reactive oxygen produced thereby without side effects and toxicity, so that the composition is useful for treating or preventing diabetes and diabetic complication. A composition for treating or preventing diabetes and diabetic complication comprises chebulic acid isolated by pulverizing Terminalia chebula, extracting the pulverized Terminalia chebula with ethanol, centrifuging the extract to obtain the supernatant, fractionating the supernatant with hexane, chloroform, ethylacetate and butanol, and subjecting the active fraction to silica gel and sephadex LH-20 column electrophoresis. The health food contains 15 wt.%, preferably 10 wt.% of the composition comprising chebulic acid.

Abstract translation: 提供包含乳酸的组合物以抑制AGE（晚期糖基化终产物）的产生并除去由此产生的活性氧而没有副作用和毒性，使得该组合物可用于治疗或预防糖尿病和糖尿病并发症。 用于治疗或预防糖尿病和糖尿病并发症的组合物包括通过粉碎Terminalia chebula分离的乳酸，用乙醇萃取粉碎的Terminalia chebula，离心提取物以获得上清液，用己烷，氯仿，乙酸乙酯和丁醇分馏上清液， 活性级分至硅胶和sephadex LH-20柱电泳。 保健食品含有15重量％，优选10重量％的包含乳酸的组合物。

公开(公告)号：KR100814368B1

公开(公告)日：2008-03-18

申请号：KR1020070038669

申请日：2007-04-20

Applicant: 고려대학교 산학협력단

Inventor： 이광원 ,  이현순

IPC: A61K31/185 ,  A61K36/185 ,  A61P3/10

Abstract: A composition comprising chebulic acid is provided to show excellent diabetes and diabetic complications inhibitory effect with secured safety due to be derived from a natural plant, thereby being widely used as a raw material of health functional foods and medicines. A composition for preventing or treating diabetes and diabetic complications comprises chebulic acid as an effective ingredient, wherein the chebulic acid is derived from Terminalia chebula. A method for preparing the composition comprises the steps of: (a) obtaining an alcohol extract of Terminalia chebula; (b) extracting the alcohol extract using at least one organic solvent in sequence in accordance with polarities; and (c) subjecting an organic solvent extract obtained from the step(b) to a gel filtration chromatography. Further, the composition for preventing or treating diabetes and diabetic complications is reacted as kinetics preventing a generation of an advanced glycation end-product.

Abstract translation: 提供一种包含乳酸的组合物，以显示优异的糖尿病和糖尿病并发症的抑制作用，具有由于天然植物得到的安全性，从而广泛用作健康功能性食品和药物的原料。 用于预防或治疗糖尿病和糖尿病并发症的组合物包含乳酸作为有效成分，其中所述乳酸衍生自Terminalia chebula。 一种制备该组合物的方法，包括以下步骤：（a）得到终止蜂蜡的醇提取物; （b）根据极性依次使用至少一种有机溶剂萃取醇提取物; 和（c）使由步骤（b）得到的有机溶剂提取物进行凝胶过滤层析。 此外，用于预防或治疗糖尿病和糖尿病并发症的组合物作为阻止产生晚期糖基化终产物的动力学反应。

公开(公告)号：KR100930927B1

公开(公告)日：2009-12-10

申请号：KR1020080022574

申请日：2008-03-11

Applicant: 고려대학교 산학협력단

Inventor： 이광원 ,  이현순 ,  박호영

IPC: A23L1/29 ,  A23L2/00 ,  A23L1/212

Abstract: 본 발명은 들깻잎으로부터 γ-GCS의 활성을 증가시키는 카페인산을 제조하는 방법, 그로부터 제조된 카페인산, 및 상기 카페인산을 포함하는 식품 조성물 및 약학 조성물에 관한 것으로서, 더욱 구체적으로는 건조 들깻잎에 대하여 증류수를 이용한 환류 냉각추출을 수행하는 단계; 상기 환류 냉각추출 단계로부터 얻어진 추출물에 대하여 원심분리를 수행하여 상등액을 얻는 단계; 상기 상등액을 여과한 여과액을 감압농축 및 동결건조시킴으로써 들깻잎 추출 분말을 얻는 단계; 상기 들깻잎 추출 분말을 복수 개의 추출용매를 사용하여 순차적으로 추출함으로써 분획물들을 제조하는 단계; 및 상기 분획물들 중 가장 높은 γ-GCS 활성을 나타내는 분획물을 분리 및 정제하는 단계를 포함하는 들깻잎으로부터 γ-GCS의 활성을 증가시키는 카페인산을 제조하는 방법, 그로부터 제조된 카페인산, 및 상기 카페인산을 포함하는 식품 조성물 및 약학 조성물에 관한 것이다.
본 발명에 따르면, 들깻잎으로부터 간편하고 저렴한 방법으로 γ-GCS의 활성을 증가시켜 간 기능을 향상시키며 강력한 항산화 효능을 갖는 카페인산을 제조할 수 있으며, 제조된 카페인산을 포함하는 식품 조성물 및 약학 조성물을 간 기능 저하 예방 및 치료에 광범위하게 활용할 수 있다.
들깻잎, 카페인산, γ-글루타밀시스테인 신테타아제, 간 보호

Abstract translation: 提供一种从绿紫苏叶生产咖啡酸改善γ-GCS活性的方法，以确保以少许成本改善肝和抗氧化效果。 从紫苏叶生产咖啡酸改善γ-GCS活性的方法包括：第一步，在蒸馏水中回流提取干燥的绿色紫苏叶; 离心提取物以从其中获得上清液的第二步骤; 第三步，过滤上清液并真空浓缩并冷冻干燥滤液以获得粉末; 第四步，依次使用多种提取溶剂提取粉末; 以及分离和精制显示高γ-GCS活性的部分的第五步骤。 在第一步中，将100g干燥的绿色紫苏叶在100-110℃的0.8-1.2L蒸馏水中提取3-4小时两次。 在第二步中，离心以4℃和7500rpm进行25-30分钟。 提取溶剂是第一，第二，第三和第四提取溶剂。

公开(公告)号：KR1020090097440A

公开(公告)日：2009-09-16

申请号：KR1020080022574

申请日：2008-03-11

Applicant: 고려대학교 산학협력단

Inventor： 이광원 ,  이현순 ,  박호영

IPC: A23L1/29 ,  A23L2/00 ,  A23L1/212

CPC classification number: A23L33/00 ,  A23L2/00 ,  A23L19/00 ,  A23V2002/00 ,  A23V2200/30 ,  A23V2250/21 ,  A61K31/192 ,  A61K36/535

Abstract: A method for producing caffeic acid improving gamma-GCS activity from green perilla leaves is provided to secure an improvement to liver and antioxidant effect at a little cost. A method for producing caffeic acid improving gamma-GCS activity from green perilla leaves comprises: a first step of reflux-extracting dried green perilla leaves in distilled water; a second step of centrifuging the extract to obtain supernate from it; a third step of filtering the supernate and vacuum-concentrating and freeze-drying the filtrate in order to obtain powder; a fourth step of extracting the powder using a plurality of extraction solvents in sequential order; and a fifth step of separating and refining the fractions showing high gamma-GCS activity. In the first step, 100g of the dried green perilla leaves are extracted in 0.8-1.2L of distilled water at 100-110°C for 3-4 hours twice. In the second step, the centrifugation is performed at 4°C and 7500rpm for 25-30 minutes. The extraction solvents are a first, second, third and fourth extraction solvent.

Abstract translation: 提供了一种从绿色紫苏叶提高咖啡酸提高γ-GCS活性的方法，以确保肝脏和抗氧化作用的改善。 一种从青紫苏叶提取咖啡酸提高γ-GCS活性的方法，包括：在蒸馏水中回收提取干燥的紫苏叶片的第一步; 离心提取物以从其中获得上清液的第二步骤; 过滤上清液并对滤液进行真空浓缩和冷冻干燥以获得粉末的第三步骤; 第四步骤，使用多个提取溶剂依次提取粉末; 以及分离和精制显示高γ-GCS活性的级分的第五步骤。 在第一步中，将100g干黄绿色叶子在0.8-1.2L蒸馏水中在100-110℃下萃取3-4小时。 在第二步中，离心在4℃和7500rpm下进行25-30分钟。 萃取溶剂是第一，第二，第三和第四萃取溶剂。

公开(公告)号：KR1020070074763A

公开(公告)日：2007-07-18

申请号：KR1020060002721

申请日：2006-01-10

Applicant: 고려대학교 산학협력단

Inventor： 이광원 ,  이현순 ,  홍충의 ,  정성훈

IPC: A61K36/68 ,  A61P17/18 ,  A61P35/00

CPC classification number: A61K36/68 ,  A61K2236/33 ,  A61K2236/51 ,  A61K2236/53

Abstract: An extraction and purification method for an effective component of a plant is provided to reduce the use amounts of extraction solvent and column resin by performing the extraction and purification processes once only, thereby reducing extraction time and enhancing economic efficiency of the extraction. The extraction and purification method for the effective component of the plant comprises the steps of: solubilizing plant powder in ethanol, heating the mixture solution with a reflux at boiling point for 2-4 hours, centrifuging the plant extract at 2000-3000 rpm for 7-15 minutes, and collecting the supernatant to prepare the crude extract; drying the crude extract through vacuum-drying or vacuum-drying and freeze-drying, and dissolving the dried crude extract in water; and subjecting the crude extract solution to ion exchange resin and eluting it with 0-100% ethanol gradients to obtain fractions, wherein the plant is Plantago asiatica Decaisne and the effective component of plant is plantamajoside.

Abstract translation: 提供植物有效成分的提取和提纯方法，通过一次进行提取和纯化过程来减少萃取溶剂和柱状树脂的使用量，从而减少萃取时间并提高萃取的经济效率。 植物有效成分的提取和纯化方法包括以下步骤：将植物粉末溶于乙醇中，加热回流沸点为2-4小时的混合溶液，以2000-3000 rpm离心植物提取物7 -15分钟，收集上清液制备粗提物; 通过真空干燥或真空干燥和冷冻干燥干燥粗提取物，并将干燥的粗提取物溶解在水中; 并将粗提物溶液进行离子交换树脂，用0-100％乙醇梯度洗脱，得到部分，其中植物是车前草，并且植物的有效成分是植物苷。