조직별, 품종별, 성장단계별 유전자 발현양상 분석을 통하여 확보한 유전자를 이용하여 성장 또는 육질이 우수한 종돈을 조기에 선발하는 방법

    公开(公告)号:KR1020090065831A

    公开(公告)日:2009-06-23

    申请号:KR1020070133339

    申请日:2007-12-18

    CPC classification number: C12Q1/6827 C12Q1/6888 C12Q2600/124

    Abstract: A specific gene for diagnosing pig growth and the diagnosis of meat quality is provided to early select excellent pig species by obtaining the specific gene related to growth and meat quality. A method for obtaining a specific gene for diagnosing growth and meat quality of pig comprises: a step of building a cDNA library using muscle and fat tissue of Sus scrofa, designing and analyzing cDNA microarray; a step of collecting muscle and fat from Sus scrofa and Yorkshire and making tissue sample; a step of cloning a gene from the tissue sample; a step of analyzing full-sequence; a step of isolating total RNA; a step of synthesizing first strand cDNA using superscript II reverse transcriptase; and a step of performing statistical analysis.

    Abstract translation: 通过获得与生长和肉质相关的具体基因,提供了诊断猪生长和肉质诊断的具体基因,以早期选择优良的猪种。 用于获得用于诊断猪的生长和肉质的特定基因的方法包括:使用Sus scrofa的肌肉和脂肪组织构建cDNA文库的步骤,设计和分析cDNA微阵列; 从Sus scrofa和Yorkshire收集肌肉和脂肪并制作组织样本的步骤; 从组织样品中克隆基因的步骤; 分析全序列的步骤; 分离总RNA的步骤; 使用上标II逆转录酶合成第一链cDNA的步骤; 以及执行统计分析的步骤。

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