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公开(公告)号：KR101445596B1
公开(公告)日：2014-10-07
申请号：KR1020120108074
申请日：2012-09-27
Applicant: 단국대학교 산학협력단 , 대한민국 (식품의약품안전처장)
CPC classification number: G01N33/12 , G01N33/533 , G01N33/54326 , G01N33/552 , G01N33/582
Abstract: 항생제 농도의 측정 방법 및 측정 키트가 제공된다. 본 발명의 일 실시예에 따른 항생제 농도의 측정 방법은, 항생제와 결합된 자성 입자를 준비하는 단계, 항생제의 항체가 하나 이상 결합된 실리카 코팅 형광 입자를 준비하는 단계, 자성 입자를 실리카 코팅 형광 입자와 반응시키는 단계, 및 반응된 실리카 코팅 형광 입자에 레이저를 조사하는 단계를 포함한다.
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公开(公告)号：KR1020140042065A
公开(公告)日：2014-04-07
申请号：KR1020120108074
申请日：2012-09-27
Applicant: 단국대학교 산학협력단 , 대한민국 (식품의약품안전처장)
CPC classification number: G01N33/12 , G01N33/533 , G01N33/54326 , G01N33/552 , G01N33/582
Abstract: Provided are a method and a kit for measuring the concentration of antibiotics. The method for measuring the concentration of antibiotics according to one embodiment of the present invention includes a step of preparing magnetic particles combined with antibiotics, a step of preparing silica coated fluorescent particles combined with one or more antibodies of antibiotics, a step of making the magnetic particles react with the fluorescent particles, and a step of making the silica coated fluorescent particles be irradiated with lasers. [Reference numerals] (AA) Start; (BB) End; (S11) Step of pre-processing antibiotics; (S12) Step of coupling an amine group on the surface of magnetic particles; (S13) Step of adding a cross linking agent to the magnetic particles in which the amine group is combined; (S14) Step of combining the magnetic particles in which the amine group is combined with antibiotics; (S15) Step of collecting the magnetic particles combined with the antibiotics by using magnetic force; (S21) Step of forming coated fluorescent particles by coating the fluorescent particles with silica; (S22) Step of combining a carboxyl group on the surface of silica coated fluorescent particles; (S23) Step of adding the cross linking agent to the silica coated fluorescent particles combined with the carboxyl group; (S24) Step of combining antibodies of the antibiotics and the silica coated fluorescent particles combined with the carboxyl group; (S25) Step of measuring the antibodies amount of the antibiotics combined with the silica coated fluorescent particles; (S3) Step of reacting the magnetic particles with the silica coated fluorescent particles; (S4) Step of collecting the magnetic particles reacting to the silica coated fluorescent particles by using a magnet; (S5) Step of measuring the concentration of the antibiotics by irradiating the silica coated fluorescent particles with a laser
Abstract translation: 提供了用于测量抗生素浓度的方法和试剂盒。 根据本发明的一个实施方案的用于测量抗生素浓度的方法包括制备与抗生素组合的磁性颗粒的步骤，制备与一种或多种抗生素抗体结合的二氧化硅涂覆的荧光颗粒的步骤，制备磁性的步骤 颗粒与荧光颗粒反应，并且用激光照射二氧化硅涂覆的荧光颗粒的步骤。 （附图标记）（AA）开始; （BB）结束; （S11）预处理抗生素的步骤; （S12）将磁性粒子表面上的胺基团偶联的工序; （S13）将交联剂加入其中胺基团的磁性颗粒中的步骤; （S14）将胺基与抗生素组合的磁性粒子组合的工序; （S15）使用磁力收集与抗生素结合的磁性粒子的步骤; （S21）通过用二氧化硅涂布荧光体颗粒来形成涂布的荧光颗粒的步骤; （S22）在二氧化硅被覆荧光体颗粒的表面上组合羧基的工序; （S23）将交联剂添加到与羧基组合的二氧化硅被覆荧光粒子上的工序; （S24）将抗生素的抗体与与羧基结合的二氧化硅被覆荧光粒子结合的步骤; （S25）测定与二氧化硅被覆荧光粒子结合的抗生素的抗体量的步骤; （S3）磁性颗粒与二氧化硅涂覆的荧光颗粒反应的步骤; （S4）使用磁铁收集与二氧化硅涂布的荧光体粒子反应的磁性粒子的工序; （S5）通过用激光照射二氧化硅被覆荧光粒子来测定抗生素浓度的步骤
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