公开(公告)号：KR101437381B1

公开(公告)日：2014-09-11

申请号：KR1020130099031

申请日：2013-08-21

Applicant: 대한민국(관리부서:국립수산과학원)

Inventor： 강정하 ,  임현정 ,  안철민 ,  이정미

IPC: C12N15/11 ,  C12Q1/68

CPC classification number: C12N15/11 ,  C12Q1/6844

Abstract: To provide an economical and effective method for discerning pacific oyster triploids, the present invention provides a multiplex kit for discerning pacific oyster triploids, wherein the multiplex kit comprises three or more primer sets selected from the group including: a primer set 1 which consists of a forward primer having a nucleic acid sequence represented by sequence number 1 and a reverse primer represented by sequence number 2; a primer set 2 which consists of a forward primer having nucleic acid sequence represented by sequence number 5 and a reverse primer having a nucleic acid sequence represented by sequence number 6; a primer set 3 which consists of a forward primer having a nucleic acid sequence represented by sequence number 7 and a reverse primer having a nucleic acid sequence represented by sequence number 8; a primer set 4 which consists of a forward primer having a nucleic acid sequence represented by sequence number 15 and a reverse primer having a nucleic acid sequence represented by sequence number 16; a primer set 5 which consists of a forward primer having a nucleic acid sequence represented by sequence number 19 and a reverse primer having a nucleic acid sequence represented by sequence number 20; and a primer set 6 which consists of a forward primer having a nucleic acid sequence represented by sequence number 21 and a reverse primer having a nucleic acid sequence represented by sequence number 22.

Abstract translation: 为了提供一种用于鉴别太平洋牡蛎三倍体的经济有效的方法，本发明提供了一种用于鉴别太平洋牡蛎三倍体的多重试剂盒，其中所述多重试剂盒包含三个或更多个选自以下的引物组：引物组1，其由 具有由序列号1表示的核酸序列的正向引物和由序列号2表示的反向引物; 引物组2，其由具有由序列号5表示的核酸序列的正向引物和具有由序列号6表示的核酸序列的反向引物组成; 引物组3，其由具有由序列号7表示的核酸序列的正向引物和具有由序列号8表示的核酸序列的反向引物组成; 引物组4，其由具有由序列号15表示的核酸序列的正向引物和具有由序列号16表示的核酸序列的反向引物组成; 引物组5，其由具有由序列号19表示的核酸序列的正向引物和具有由序列号20表示的核酸序列的反向引物组成; 和引物组6，其由具有由序列号21表示的核酸序列的正向引物和具有由序列号22表示的核酸序列的反向引物组成。