公开(公告)号：KR1020130016790A

公开(公告)日：2013-02-19

申请号：KR1020110078907

申请日：2011-08-09

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 차상호 ,  소병재 ,  나진주 ,  이명헌 ,  이금실 ,  이희수 ,  안영수

IPC: C12N5/0775 ,  C12Q1/02

Abstract: PURPOSE: A method for preparing pluripotent stem cells from canine umbilical cord matrix is provided to express one or more differentiation markers(Oct-3/4, Sox-2, SSEA-4, Nanog). CONSTITUTION: A method for preparing umbilical cord matrix-derived mesenchymal stem cells comprises: a step of collecting an umbilical cord matrix and removing amnion from the umbilical cord matrix; a step of treating the umbilical cord matrix with trypsin/EDTA at 36-38°C for 30 minutes to one hour; a step of removing umbilical artery and umbilical vein from the umbilical cord matrix; a step of treating the umbilical cord matrix with collagenase at 36-38°C for 30 minutes to 2 hours and collecting single cells; and a step of culturing and collecting the pluripotent mesenchymal stem cells in a medium. The medium contains bFGF(Basic Fibroblast Growth Factor).

Abstract translation: 目的：提供从犬脐带基质制备多能干细胞的方法，以表达一种或多种分化标志物（Oct-3/4，Sox-2，SSEA-4，Nanog）。 构成：制备脐带基质的间充质干细胞的方法包括：收集脐带基质并从脐带基质中除去羊膜的步骤; 用胰蛋白酶/ EDTA在36-38℃处理脐带基质30分钟至1小时的步骤; 从脐带基质中去除脐动脉和脐静脉的步骤; 用胶原酶在36-38℃处理脐带基质30分钟至2小时并收集单细胞的步骤; 以及在培养基中培养和收集多能间充质干细胞的步骤。 培养基含有bFGF（碱性成纤维细胞生长因子）。