公开(公告)号：KR1020130063850A

公开(公告)日：2013-06-17

申请号：KR1020110130447

申请日：2011-12-07

Applicant: 대한민국(농림축산식품부 농림축산검역본부장)

Inventor： 신연경 ,  송재영 ,  최은진 ,  조우얀 ,  표현미

IPC: C12N7/01 ,  C12N15/44 ,  A61K39/145 ,  A61P31/16

Abstract: PURPOSE: A recombinant baculovirus is provided to protect a swinery from novel swine-origin influenza A(H1N1) and to prevent new or variant virus expressed in a pig. CONSTITUTION: A recombinant baculovirus is prepared by inserting a gene which expresses HA(hemagglutinin), a gene which expresses NA(neuraminidase), and a gene which expresses M1(matrix 1) protein. The HA expressing gene is a gene(GeneBank FJ966082) which expresses HA of influenza virus A/California/04/2009(CA09). The NA expressing gene is a gene(GeneBank FJ966084) which expresses NA of influenzavirus A/California/04/2009(CA09). The M1 protein expressing gene is a gene(GeneBank FJ969513) which expresses M1 protein of influenza virus A/California/04/2009(CA09). A VLP(Virus-Like Particle) for suppressing swine influenza viral infection contains the recombinant baculovirus. A vaccine for suppressing swine influenza viral infection contains the VLP. [Reference numerals] (AA) Tn7L,Tn7R sequence homologous recombination for Baculovirus

Abstract translation: 目的：提供重组杆状病毒以保护猪只免受猪源甲型H1N1流感病毒侵袭，并防止在猪中表达的新病毒或变异病毒。 构成：通过插入表达HA（血凝素）的基因，表达NA（神经氨酸酶）的基因和表达M1（基质1）蛋白质的基因来制备重组杆状病毒。 HA表达基因是表达流感病毒A / California / 04/2009（CA09）的HA的基因（GeneBank FJ966082）。 NA表达基因是表达流感病毒A / California / 04/2009（CA09）的NA的基因（GeneBank FJ966084）。 M1蛋白表达基因是表达流感病毒A / California / 04/2009（CA09）的M1蛋白的基因（GeneBank FJ969513）。 用于抑制猪流感病毒感染的VLP（病毒样颗粒）含有重组杆状病毒。 用于抑制猪流感病毒感染的疫苗包含VLP。 （AA）Tn7L，Tn7R序列同源重组杆状病毒