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    1.
    发明公开

    公开(公告)号:KR1020100132321A

    公开(公告)日:2010-12-17

    申请号:KR1020090051084

    申请日:2009-06-09

    CPC classification number: C12Q1/6827 C12N15/11 C12Q2600/156

    Abstract: PURPOSE: A method for selecting backfat thickness of a pig is provided to measure accurate genetic ability and to raise pig with high quality. CONSTITUTION: A SNP of leptin receptor gene(LEPR) is used as a molecular marker for evaluating phenotype of a pig. The SNPs are -200, -124, -25 or +161 loci mutant based on transcription initiation base(+1). The phenotype is backfat thickness of pig. A microarray for selecting the backfat thickness contains the molecular marker. A method for selecting backfat thickness of pig comprises: a step of obtaining a biological sample having a LEPR gene; a step of amplifying the LEPT gene by PCR; a step of detecting SNP from the PCR product; and a step of selecting SNP having significance in backfat thickness.

    Abstract translation: 目的:提供一种选择猪背板厚度的方法,以测量准确的遗传能力,提高猪的质量。 构成:使用瘦素受体基因(LEPR)的SNP作为评估猪表型的分子标记。 基于转录起始碱基(+1),SNP为-200,-124,-25或+161位点突变体。 表型为猪背背厚度。 用于选择背at厚度的微阵列包含分子标记。 一种选择猪背板厚度的方法,包括:获得具有LEPR基因的生物样品的步骤; 通过PCR扩增LEPT基因的步骤; 从PCR产物检测SNP的步骤; 以及选择具有背at厚度的SNP的步骤。

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