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公开(公告)号:KR100435153B1
公开(公告)日:2004-06-14
申请号:KR1020010041698
申请日:2001-07-11
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/68
Abstract: PURPOSE: PCR primers for detecting a DNA fragment specific to Phellinus linteus are provided, thereby deciding can be rapidly and correctly whether a mushroom is Phellinus linteus. CONSTITUTION: The DNA fragment specific to Phellinus linteus has the nucleotide sequence of SEQ ID NO: 2, wherein the DNA fragment specific to Phellinus linteus is derived from a mycelium or a fruit body of Phellinus linteus. The PCR primers for detecting the DNA fragment specific to Phellinus linteus contains 10 to 40 mer in the nucleotide sequence of SEQ ID NO: 2, wherein the PCR primers include PLSPF1 having the nucleotide sequence of SEQ ID NO: 3, PLSPF2 having the nucleotide sequence of SEQ ID NO: 4, PLSPR1 having the nucleotide sequence of SEQ ID NO: 5, and PLSPR2 having the nucleotide sequence of SEQ ID NO: 6.
Abstract translation: 目的:提供用于检测桑黄特异性DNA片段的PCR引物,由此可以快速准确地判定蘑菇是否是桑黄。 构成:特异于Phellinus linteus的DNA片段具有SEQ ID NO:2的核苷酸序列,其中特异于Phellinus linteus的DNA片段来源于桑黄的菌丝体或果实体。 用于检测特异于桑黄的DNA片段的PCR引物在SEQ ID NO:2的核苷酸序列中含有10至40聚体,其中PCR引物包括具有SEQ ID NO:3的核苷酸序列的PLSPF1,具有该核苷酸序列的PLSPF2 SEQ ID NO:4的核苷酸序列的PLSPR1,具有SEQ ID NO:5的核苷酸序列的PLSPR1和具有SEQ ID NO:6的核苷酸序列的PLSPR2。
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公开(公告)号:KR1020030006126A
公开(公告)日:2003-01-23
申请号:KR1020010041698
申请日:2001-07-11
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/68
Abstract: PURPOSE: PCR primers for detecting a DNA fragment specific to Phellinus linteus are provided, thereby deciding can be rapidly and correctly whether a mushroom is Phellinus linteus. CONSTITUTION: The DNA fragment specific to Phellinus linteus has the nucleotide sequence of SEQ ID NO: 2, wherein the DNA fragment specific to Phellinus linteus is derived from a mycelium or a fruit body of Phellinus linteus. The PCR primers for detecting the DNA fragment specific to Phellinus linteus contains 10 to 40 mer in the nucleotide sequence of SEQ ID NO: 2, wherein the PCR primers include PLSPF1 having the nucleotide sequence of SEQ ID NO: 3, PLSPF2 having the nucleotide sequence of SEQ ID NO: 4, PLSPR1 having the nucleotide sequence of SEQ ID NO: 5, and PLSPR2 having the nucleotide sequence of SEQ ID NO: 6.
Abstract translation: 目的:提供用于检测特异于番茄的DNA片段的PCR引物,从而可以快速准确地确定蘑菇是否为桑黄素。 构成:对桑黄素特异性的DNA片段具有SEQ ID NO:2的核苷酸序列,其中对桑黄素特异性的DNA片段来源于桑皮的菌丝体或果实体。 用于检测对桑黄素特异性的DNA片段的PCR引物在SEQ ID NO:2的核苷酸序列中含有10至40个聚体,其中PCR引物包括具有SEQ ID NO:3的核苷酸序列的PLSPF1,具有核苷酸序列的PLSPF2 具有SEQ ID NO:5的核苷酸序列的PLSPR1和具有SEQ ID NO:6的核苷酸序列的PLSPR2。
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