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1.发明公开
公开(公告)号：KR1020030004864A
公开(公告)日：2003-01-15
申请号：KR1020010040594
申请日：2001-07-07
Applicant: 대한민국(농촌진흥청장)
IPC: C12N1/00
Abstract: PURPOSE: Provided are a selective culture medium composition for pure acido-halophilic microorganisms viable in semi-fluid conditions and a manufacturing method the selection medium using the same composition, thereby culturing the microorganisms with higher growth rate than in an MRS medium or a YM medium. CONSTITUTION: The selective culture medium composition for pure acido-halophilic microorganisms viable in semi-fluid conditions comprises 3 to 7 g/l of yeast extract, 1.5 to 4 g/l of malt extract, 1.5 to 4 g/l of meat extract, 8 to 12 g/l of tryptone or peptone, 15 to 25 g/l of glucose or lactose, 0.5 to 1.5 g/l of polysorbate 80, 1 to 3 g/l of citric acid or malic acid, 25 to 35 g/l of sodium chloride, and 7 to 9 g/l of agar, wherein 25 to 35 ml/l of glycerol is optionally added to the composition. The method for producing the selective culture medium comprises the steps of: mixing yeast extract, malt extract, meat extract, tryptone or peptone, glucose or lactose, polysorbate 80, citric acid or malic acid, sodium chloride, and agar and inserting the mixture into a flask; adding water into the mixture; slightly heating the mixture; adding glycerol into the heated mixture; and sealing the flask and sterilizing the mixture.
Abstract translation: 目的：提供一种在半流体条件下可行的纯酸性嗜酸性微生物的选择性培养基组合物，以及使用相同组成的选择培养基的制造方法，从而培养生长速度高于MRS培养基或YM培养基的微生物 。 构成：在半流体条件下存在的纯酸性嗜酸性微生物的选择性培养基组合物包含3至7g / l酵母提取物，1.5至4g / l麦芽提取物，1.5至4g / l肉提取物， 8至12g / l胰蛋白胨或蛋白胨，15至25g / l葡萄糖或乳糖，0.5至1.5g / l聚山梨醇酯80,1至3g / l柠檬酸或苹果酸，25至35g / l的氯化钠和7至9g / l的琼脂，其中25至35ml / l的甘油任选加入到组合物中。 制备选择性培养基的方法包括以下步骤：混合酵母提取物，麦芽提取物，肉提取物，胰蛋白胨或蛋白胨，葡萄糖或乳糖，聚山梨酯80，柠檬酸或苹果酸，氯化钠和琼脂，并将混合物 一个烧瓶 向混合物中加入水; 稍微加热混合物; 将甘油加入加热的混合物中; 并密封烧瓶并对混合物进行灭菌。
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公开(公告)号：KR101211560B1
公开(公告)日：2012-12-12
申请号：KR1020090099886
申请日：2009-10-20
Applicant: 대한민국(농촌진흥청장)
CPC classification number: Y02P60/871
Abstract: 본발명은청보리사일리지를이용한양돈용풀 발효사료및 이의제조방법에관한것으로서, 더욱상세하게는기존배합사료내 섬유소함량부족에따른심한배고픔현상, 대변량부족으로인한변비, 대장으로유입되는섬유소부족으로인한이상발효, 각종스트레스에의한면역력저하등을해소하여돼지의생산성을높일목적으로청보리사일리지를이용하여섬유질과유산균이풍부히함유되도록제조된양돈용풀 발효사료및 이의제조방법에관한것이다.
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Patent Agency Ranking
公开(公告)号：KR1020040080172A
公开(公告)日：2004-09-18
申请号：KR1020030015051
申请日：2003-03-11
Applicant: 대한민국(농촌진흥청장)
IPC: A23K1/165
CPC classification number: A23K10/14 , A23K10/18 , A23K20/189 , A23K50/10 , C12R1/00
Abstract: PURPOSE: To promote the secretion rate of digestive enzymes from anaerobic microbes in ruminant stomachs, thereby increasing the amount of milk production. CONSTITUTION: The agent is prepared by (a) mixing a non ionic surfactant and an acrylic acid polymer; (b) mixing the mixture with an alkaline ionic water(pH 11) at volume ratio of 1 to 10 to prepare a diluted solution; and (c) mixing the diluted solution with additives at rate of 1 to 1 (v/w). The feed composition is characterized in that it comprises 0.001 to 20 wt.% of the agent regarding the total weight of the feed composition.
Abstract translation: 目的：促进反刍动物胃中厌氧微生物消化酶的分泌，从而增加产奶量。 构成：通过（a）混合非离子表面活性剂和丙烯酸聚合物制备试剂; （b）混合物与体积比为1至10的碱性离子水（pH11）混合以制备稀释溶液; 和（c）以1至1（v / w）的速率将稀释的溶液与添加剂混合。 饲料组合物的特征在于其包含占试剂组合物总重的0.001-20重量％的试剂。
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公开(公告)号：KR1020040080171A
公开(公告)日：2004-09-18
申请号：KR1020030015050
申请日：2003-03-11
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/34
Abstract: PURPOSE: A kit for measuring rumen digestibility is provided, thereby easily measuring the rumen digestibility without surgery operation, and reducing the cost for measuring rumen digestibility. CONSTITUTION: The kit for measuring rumen digestibility comprises a hydrolysis enzyme having standardized titer, antibiotics and a buffering material, wherein the standardization is carried out by mixing enzymes to have the condition consisting of the same distribution, kinds and titers of enzymes as the real rumen; the hydrolysis enzyme is one or more selected from alpha-amylase, beta-amylase, amyloglucosidase, cellulase, hemicellulase and protease; the antibiotics is one or more selected from penicillin-G, streptomycin and chloramphenicol; and the buffering material is sodium bicarbonate.
Abstract translation: 目的：提供用于测量瘤胃消化率的试剂盒，从而轻松测量瘤胃消化率而无需手术操作，并降低测量瘤胃消化率的成本。 构成：用于测量瘤胃消化率的试剂盒包括具有标准滴定度，抗生素和缓冲材料的水解酶，其中标准化是通过混合酶进行的，以使酶具有与真正的瘤胃相同的分布，种类和滴度 ; 水解酶是选自α-淀粉酶，β-淀粉酶，淀粉葡糖苷酶，纤维素酶，半纤维素酶和蛋白酶中的一种或多种; 抗生素是选自青霉素G，链霉素和氯霉素中的一种或多种; 缓冲材料为碳酸氢钠。
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公开(公告)号：KR1020110042991A
公开(公告)日：2011-04-27
申请号：KR1020090099886
申请日：2009-10-20
Applicant: 대한민국(농촌진흥청장)
CPC classification number: Y02P60/871
Abstract: PURPOSE: Grass fermentation pig feed using green barley silage, and a producing method thereof are provided to prevent symptoms including constipation and the shortage of fecal quantity of pigs caused by the lack of edible fibers in the feed. CONSTITUTION: A producing method of grass fermentation pig feed using green barley silage comprises the following steps: cutting and crushing the green barley silage, and mixing with mixed feed; adding treber and molasses into liquid beer yeast, for obtaining a liquid material; mixing the liquid material, probiotics, and the mixture of the mixed feed and the green barley silage; seal-packing the mixture; and naturally fermenting the mixture for 5~15days.
Abstract translation: 目的：提供使用绿色大麦青贮饲料的草发酵猪饲料及其制备方法，以防止饲料中缺乏可食用纤维引起的便秘和粪便数量不足等症状。 构成：使用绿色大麦青贮饲料的草发酵猪饲料的生产方法包括以下步骤：切碎并粉碎青大麦青贮饲料，并与混合饲料混合; 将柠檬和糖蜜加入液体啤酒酵母中，得到液体物质; 混合液体材料，益生菌以及混合饲料和绿色大麦青贮饲料的混合物; 密封包装混合物; 并自然发酵混合物5〜15天。
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公开(公告)号：KR100496016B1
公开(公告)日：2005-06-16
申请号：KR1020030015050
申请日：2003-03-11
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/34
Abstract: 본 발명은 반추위내에서 사료분해에 직접적으로 관여하는 가수분해 효소의 종류와 역가 및 분포체계들을 축종별로 구명하고, 사료급여 후 경과시간별로 측정한 다음 반추위내 효소의 역가를 표준화시켜 산업적으로 판매되고 있는 효소들을 이용하여 반추위와 동일한 역가로 혼합하고 미생물의 오염을 방지하기 위하여 항생제를 첨가하여 제조한 반추위 동물의 소화율 측정용 키트에 관한 것이다.
본 발명은 여러 가지 사료들의 반추위내 소화율을 측정한 결과 전통적으로 사용하고 있는 위액을 이용한 in vitro 방법이나 누관을 설치한 동물을 직접 이용하는 in situ 방법과 유사한 결과를 얻었다. 이러한 결과는 사료의 소화율 측정에 투여되는 시간이나 장비 및 노동력을 크게 줄일 수 있고 동물이나 동물에 대한 수술작업이 전혀 필요치 않아 손쉽고 경제적인 방법으로서 반추위 소화율 분석비용을 1/3 이상 감소시킬 수 있다.-
公开(公告)号：KR100445163B1
公开(公告)日：2004-08-21
申请号：KR1020010040594
申请日：2001-07-07
Applicant: 대한민국(농촌진흥청장)
IPC: C12N1/00
Abstract: PURPOSE: Provided are a selective culture medium composition for pure acido-halophilic microorganisms viable in semi-fluid conditions and a manufacturing method the selection medium using the same composition, thereby culturing the microorganisms with higher growth rate than in an MRS medium or a YM medium. CONSTITUTION: The selective culture medium composition for pure acido-halophilic microorganisms viable in semi-fluid conditions comprises 3 to 7 g/l of yeast extract, 1.5 to 4 g/l of malt extract, 1.5 to 4 g/l of meat extract, 8 to 12 g/l of tryptone or peptone, 15 to 25 g/l of glucose or lactose, 0.5 to 1.5 g/l of polysorbate 80, 1 to 3 g/l of citric acid or malic acid, 25 to 35 g/l of sodium chloride, and 7 to 9 g/l of agar, wherein 25 to 35 ml/l of glycerol is optionally added to the composition. The method for producing the selective culture medium comprises the steps of: mixing yeast extract, malt extract, meat extract, tryptone or peptone, glucose or lactose, polysorbate 80, citric acid or malic acid, sodium chloride, and agar and inserting the mixture into a flask; adding water into the mixture; slightly heating the mixture; adding glycerol into the heated mixture; and sealing the flask and sterilizing the mixture.
Abstract translation: 目的：提供一种用于在半流体条件下存活的纯酸 - 嗜盐微生物的选择性培养基组合物和一种使用相同组合物的选择培养基的制备方法，由此培养具有比在MRS培养基或YM培养基中更高生长速率的微生物 。 构成：用于在半流体条件下存活的纯酸 - 嗜盐微生物的选择性培养基组合物包含3至7g / l酵母提取物，1.5至4g / l麦芽提取物，1.5至4g / l肉提取物， 8至12克/升胰蛋白胨或蛋白胨，15至25克/升葡萄糖或乳糖，0.5至1.5克/升聚山梨酯80,1至3克/升柠檬酸或苹果酸，25至35克/ l的氯化钠和7-9g / l的琼脂，其中任选地将25-35ml / l的甘油加入到组合物中。 生产选择性培养基的方法包括以下步骤：将酵母提取物，麦芽提取物，肉膏，胰蛋白胨或蛋白胨，葡萄糖或乳糖，聚山梨酯80，柠檬酸或苹果酸，氯化钠和琼脂混合并将混合物插入 烧瓶; 将水加入混合物中; 稍微加热混合物; 向加热的混合物中加入甘油; 并密封烧瓶并将混合物灭菌。
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