公开(公告)号：KR1020040080172A

公开(公告)日：2004-09-18

申请号：KR1020030015051

申请日：2003-03-11

Applicant: 대한민국(농촌진흥청장)

Inventor： 이성실 ,  김현섭 ,  신기준 ,  정일병 ,  이병석 ,  이왕식 ,  김창현 ,  정완태

IPC: A23K1/165

CPC classification number: A23K10/14 ,  A23K10/18 ,  A23K20/189 ,  A23K50/10 ,  C12R1/00

Abstract: PURPOSE: To promote the secretion rate of digestive enzymes from anaerobic microbes in ruminant stomachs, thereby increasing the amount of milk production. CONSTITUTION: The agent is prepared by (a) mixing a non ionic surfactant and an acrylic acid polymer; (b) mixing the mixture with an alkaline ionic water(pH 11) at volume ratio of 1 to 10 to prepare a diluted solution; and (c) mixing the diluted solution with additives at rate of 1 to 1 (v/w). The feed composition is characterized in that it comprises 0.001 to 20 wt.% of the agent regarding the total weight of the feed composition.

Abstract translation: 目的：促进反刍动物胃中厌氧微生物消化酶的分泌，从而增加产奶量。 构成：通过（a）混合非离子表面活性剂和丙烯酸聚合物制备试剂; （b）混合物与体积比为1至10的碱性离子水（pH11）混合以制备稀释溶液; 和（c）以1至1（v / w）的速率将稀释的溶液与添加剂混合。 饲料组合物的特征在于其包含占试剂组合物总重的0.001-20重量％的试剂。

公开(公告)号：KR1020040080171A

公开(公告)日：2004-09-18

申请号：KR1020030015050

申请日：2003-03-11

Applicant: 대한민국(농촌진흥청장)

Inventor： 이성실 ,  신기준 ,  정일병 ,  이병석 ,  이왕식 ,  김창현 ,  정완태

IPC: C12Q1/34

Abstract: PURPOSE: A kit for measuring rumen digestibility is provided, thereby easily measuring the rumen digestibility without surgery operation, and reducing the cost for measuring rumen digestibility. CONSTITUTION: The kit for measuring rumen digestibility comprises a hydrolysis enzyme having standardized titer, antibiotics and a buffering material, wherein the standardization is carried out by mixing enzymes to have the condition consisting of the same distribution, kinds and titers of enzymes as the real rumen; the hydrolysis enzyme is one or more selected from alpha-amylase, beta-amylase, amyloglucosidase, cellulase, hemicellulase and protease; the antibiotics is one or more selected from penicillin-G, streptomycin and chloramphenicol; and the buffering material is sodium bicarbonate.

Abstract translation: 目的：提供用于测量瘤胃消化率的试剂盒，从而轻松测量瘤胃消化率而无需手术操作，并降低测量瘤胃消化率的成本。 构成：用于测量瘤胃消化率的试剂盒包括具有标准滴定度，抗生素和缓冲材料的水解酶，其中标准化是通过混合酶进行的，以使酶具有与真正的瘤胃相同的分布，种类和滴度 ; 水解酶是选自α-淀粉酶，β-淀粉酶，淀粉葡糖苷酶，纤维素酶，半纤维素酶和蛋白酶中的一种或多种; 抗生素是选自青霉素G，链霉素和氯霉素中的一种或多种; 缓冲材料为碳酸氢钠。

公开(公告)号：KR100399570B1

公开(公告)日：2003-09-29

申请号：KR1020010007897

申请日：2001-02-16

Applicant: 대한민국(농촌진흥청장)

Inventor： 이왕식 ,  이병석 ,  이상철 ,  신기준 ,  정일병 ,  이영석

IPC: A23K1/00

Abstract: PURPOSE: An alternative device for roughage obtained by using polypropylene as a core rod and then planting polymeric fibers in the core rod is provided which reduces the amount of roughage intake and feces and improves productivity. CONSTITUTION: Polymeric fibers(3) having a length of 10 to 40mm are planted in a core rod(2) using a steel core(5) for fixing to form an alternative device(1) for roughage. The diameter of the core rod has a diameter of 10 to 30mm to easily feed to ruminant animals and the polymeric fibers have a specific gravity of 0.80 to 0.90 for stimulation in the rumen of the animal.

Abstract translation: 目的：提供一种用聚丙烯作为芯棒，然后在芯棒中植入聚合物纤维的粗饲料替代装置，可减少粗饲料摄入量和粪便量，并提高生产率。 组成：长度为10至40mm的聚合物纤维（3）使用钢芯（5）植入芯棒（2）中以固定以形成用于粗饲料的替代装置（1）。 芯棒的直径为10-30mm，易于喂养反刍动物，聚合物纤维的比重为0.80-0.90，用于动物瘤胃的刺激。

公开(公告)号：KR1020030004865A

公开(公告)日：2003-01-15

申请号：KR1020010040595

申请日：2001-07-07

Applicant: 대한민국(농촌진흥청장)

Inventor： 이성실 ,  신기준 ,  정일병 ,  이병석 ,  정완태 ,  이왕식

IPC: A23K1/10

CPC classification number: A23K10/18 ,  A23K20/189

Abstract: PURPOSE: Provided is a fermenting agent for hygienically producing safe feeds from food wastes, thereby preventing the growth of pathogenic microorganisms, increasing enzyme activity and reduced sugar level and maximizing coefficient of utilization of food wastes. CONSTITUTION: The fermenting agent for hygienically producing safe feeds from food wastes is characteristically composed of 5-15 wt.% of microbial powder and 85-95 wt.% of excipient. It further contains 5-15 part by weight of oligosaccharide, referred to 100 part by weight of the fermenting agent. Wherein the microbial powder is prepared by mixing at least one selected from the group consisting of Bacillus circulans(ATCC 9500), Bacillus megaterium(ATCC 10778), Lactobacillus amylophilus(ATCC 49845), Weissella confusa(ATCC 14434), Bifidus sp.(ATCC 11863), Candida utilis(ATCC 9950) and Candida sp.(ATCC 90238); the excipient is at least one selected from the group consisting of corn flour, rice bran and wheat bran; the oligosaccharide is at least one selected from the group consisting of glucomannan-oligosaccharide, inulo-oligosaccharide, fructo-oligosaccharide and mannan-oligosaccharide.

Abstract translation: 目的：提供一种用于卫生生产食品废物安全饲料的发酵剂，从而防止病原微生物的生长，增加酶活性，降低糖含量，最大限度地利用食物废物。 构成：用于卫生地生产来自食品废物的安全饲料的发酵剂的特征在于5-15重量％的微生物粉末和85-95重量％的赋形剂。 它还含有5-15重量份的寡糖，称为100重量份的发酵剂。 其中微生物粉末是通过混合选自环状芽胞杆菌（ATCC 9500），巨大芽孢杆菌（Bacillus megaterium）（ATCC 10778），布氏乳杆菌（ATCC 49845），We，（Weissella confusa）（ATCC 14434），双歧杆菌（Bifidus sp。 11863），产朊假丝酵母（ATCC 9950）和假丝酵母属（ATCC 90238）; 赋形剂是选自玉米粉，米糠和麦麸中的至少一种; 所述寡糖是选自由葡甘露聚糖 - 低聚糖，全低聚糖，果寡糖和甘露聚寡糖组成的组中的至少一种。

公开(公告)号：KR100496016B1

公开(公告)日：2005-06-16

申请号：KR1020030015050

申请日：2003-03-11

Applicant: 대한민국(농촌진흥청장)

Inventor： 이성실 ,  신기준 ,  정일병 ,  이병석 ,  이왕식 ,  김창현 ,  정완태

IPC: C12Q1/34

Abstract: 본 발명은 반추위내에서 사료분해에 직접적으로 관여하는 가수분해 효소의 종류와 역가 및 분포체계들을 축종별로 구명하고, 사료급여 후 경과시간별로 측정한 다음 반추위내 효소의 역가를 표준화시켜 산업적으로 판매되고 있는 효소들을 이용하여 반추위와 동일한 역가로 혼합하고 미생물의 오염을 방지하기 위하여 항생제를 첨가하여 제조한 반추위 동물의 소화율 측정용 키트에 관한 것이다.
본 발명은 여러 가지 사료들의 반추위내 소화율을 측정한 결과 전통적으로 사용하고 있는 위액을 이용한 in vitro 방법이나 누관을 설치한 동물을 직접 이용하는 in situ 방법과 유사한 결과를 얻었다. 이러한 결과는 사료의 소화율 측정에 투여되는 시간이나 장비 및 노동력을 크게 줄일 수 있고 동물이나 동물에 대한 수술작업이 전혀 필요치 않아 손쉽고 경제적인 방법으로서 반추위 소화율 분석비용을 1/3 이상 감소시킬 수 있다.

公开(公告)号：KR100445163B1

公开(公告)日：2004-08-21

申请号：KR1020010040594

申请日：2001-07-07

Applicant: 대한민국(농촌진흥청장)

Inventor： 이성실 ,  신기준 ,  정일병 ,  이병석 ,  김창현 ,  정완태 ,  이왕식

IPC: C12N1/00

Abstract: PURPOSE: Provided are a selective culture medium composition for pure acido-halophilic microorganisms viable in semi-fluid conditions and a manufacturing method the selection medium using the same composition, thereby culturing the microorganisms with higher growth rate than in an MRS medium or a YM medium. CONSTITUTION: The selective culture medium composition for pure acido-halophilic microorganisms viable in semi-fluid conditions comprises 3 to 7 g/l of yeast extract, 1.5 to 4 g/l of malt extract, 1.5 to 4 g/l of meat extract, 8 to 12 g/l of tryptone or peptone, 15 to 25 g/l of glucose or lactose, 0.5 to 1.5 g/l of polysorbate 80, 1 to 3 g/l of citric acid or malic acid, 25 to 35 g/l of sodium chloride, and 7 to 9 g/l of agar, wherein 25 to 35 ml/l of glycerol is optionally added to the composition. The method for producing the selective culture medium comprises the steps of: mixing yeast extract, malt extract, meat extract, tryptone or peptone, glucose or lactose, polysorbate 80, citric acid or malic acid, sodium chloride, and agar and inserting the mixture into a flask; adding water into the mixture; slightly heating the mixture; adding glycerol into the heated mixture; and sealing the flask and sterilizing the mixture.

Abstract translation: 目的：提供一种用于在半流体条件下存活的纯酸 - 嗜盐微生物的选择性培养基组合物和一种使用相同组合物的选择培养基的制备方法，由此培养具有比在MRS培养基或YM培养基中更高生长速率的微生物 。 构成：用于在半流体条件下存活的纯酸 - 嗜盐微生物的选择性培养基组合物包含3至7g / l酵母提取物，1.5至4g / l麦芽提取物，1.5至4g / l肉提取物， 8至12克/升胰蛋白胨或蛋白胨，15至25克/升葡萄糖或乳糖，0.5至1.5克/升聚山梨酯80,1至3克/升柠檬酸或苹果酸，25至35克/ l的氯化钠和7-9g / l的琼脂，其中任选地将25-35ml / l的甘油加入到组合物中。 生产选择性培养基的方法包括以下步骤：将酵母提取物，麦芽提取物，肉膏，胰蛋白胨或蛋白胨，葡萄糖或乳糖，聚山梨酯80，柠檬酸或苹果酸，氯化钠和琼脂混合并将混合物插入 烧瓶; 将水加入混合物中; 稍微加热混合物; 向加热的混合物中加入甘油; 并密封烧瓶并将混合物灭菌。

公开(公告)号：KR100375673B1

公开(公告)日：2003-03-15

申请号：KR1020000004429

申请日：2000-01-29

Applicant: 대한민국(농촌진흥청장)

Inventor： 이현정 ,  이상철 ,  김진욱 ,  신기준 ,  최윤재 ,  강승하 ,  복진덕

IPC: C12N15/63

Abstract: PURPOSE: Provided is a recombinant vector pGAP-phyA including phytase gene coding myo-inositol hexaphosphate phosphohydrolase derived from gram-positive bacteria, Enterobactor sp.4. And transformant Pichia pastoris pGAP-phyA is also provided, which can secret myo-inositol hexaphosphate phosphohydrolase continuously, and is useful as a feed additive. CONSTITUTION: A recombinant vector pGAP-phyA including phytase gene coding myo-inositol hexaphosphate phosphohydrolase is prepared by the steps of: (a) separating myo-inositol hexaphosphate phosphohydrolase chromosome derived from Enterobactor sp.4; (b) cloning and sequencing the base sequence of the gene coding myo-inositol hexaphosphate phosphohydrolase originated from gram-positive bacteria, Enterobactor sp.4; (c) constructing an expression vector including a gene fragment for coding myo-inositol hexaphosphate phosphohydrolase; (d) transforming Pichia pastoris GS 115 yeast strain with the vector; and (e) selecting a transformant.

Abstract translation: 目的：提供一种重组载体pGAP-phyA，其包括编码肌酸六磷酸磷酸水解酶的肌醇六磷酸酶基因，所述肌醇六磷酸磷酸水解酶来源于革兰氏阳性细菌Enterobactor sp.4。 还提供了转化体巴斯德毕赤酵母pGAP-phyA，其可以连续分泌肌醇六磷酸磷酸水解酶，并且可用作饲料添加剂。 构成：通过以下步骤制备包含编码肌醇六磷酸磷酸水解酶的植酸酶基因的重组载体pGAP-phyA：（a）分离源自Enterobactor sp.4的肌醇六磷酸磷酸水解酶染色体; （b）克隆和测序编码源自革兰氏阳性细菌肌醇六磷酸磷酸水解酶的基因的碱基序列，Enterobactor sp.4; （c）构建包含编码肌肉六磷酸磷酸水解酶的基因片段的表达载体; （d）用载体转化巴斯德毕赤酵母GS115酵母菌株; 和（e）选择转化体。

公开(公告)号：KR1020010076962A

公开(公告)日：2001-08-17

申请号：KR1020000004429

申请日：2000-01-29

Applicant: 대한민국(농촌진흥청장)

Inventor： 이현정 ,  이상철 ,  김진욱 ,  신기준 ,  최윤재 ,  강승하 ,  복진덕

IPC: C12N15/63

CPC classification number: C12N15/63 ,  A23K10/16 ,  A61K35/741

Abstract: PURPOSE: Provided is a recombinant vector pGAP-phyA including phytase gene coding myo-inositol hexaphosphate phosphohydrolase derived from gram-positive bacteria, Enterobactor sp.4. And transformant Pichia pastoris pGAP-phyA is also provided, which can secret myo-inositol hexaphosphate phosphohydrolase continuously, and is useful as a feed additive. CONSTITUTION: A recombinant vector pGAP-phyA including phytase gene coding myo-inositol hexaphosphate phosphohydrolase is prepared by the steps of: (a) separating myo-inositol hexaphosphate phosphohydrolase chromosome derived from Enterobactor sp.4; (b) cloning and sequencing the base sequence of the gene coding myo-inositol hexaphosphate phosphohydrolase originated from gram-positive bacteria, Enterobactor sp.4; (c) constructing an expression vector including a gene fragment for coding myo-inositol hexaphosphate phosphohydrolase; (d) transforming Pichia pastoris GS 115 yeast strain with the vector; and (e) selecting a transformant.

Abstract translation: 目的：提供一种重组载体pGAP-phyA，其中包括植酸酶基因，其编码来自革兰氏阳性菌的Entero反应器sp.4的肌醇六磷酸磷酸水解酶。 还提供了转化体巴斯德毕赤酵母pGAP-phyA，其可以连续分泌肌醇六磷酸磷酸水解酶，并且可用作饲料添加剂。 构成：通过以下步骤制备包含编码肌醇六磷酸磷酸水解酶的肌醇六磷酸酶基因的重组载体pGAP-phyA：（a）分离源自Enterobactor sp.4的肌醇六磷酸磷酸水解酶染色体; （b）克隆和测序来源于革兰氏阳性菌的Enterobactor sp.4的编码肌醇六磷酸磷酸水解酶的基因的碱基序列; （c）构建包含编码肌醇六磷酸磷酸水解酶的基因片段的表达载体; （d）用载体转化巴斯德毕赤酵母GS 115酵母菌株; 和（e）选择转化体。

公开(公告)号：KR100435154B1

公开(公告)日：2004-06-14

申请号：KR1020010040595

申请日：2001-07-07

Applicant: 대한민국(농촌진흥청장)

Inventor： 이성실 ,  신기준 ,  정일병 ,  이병석 ,  정완태 ,  이왕식

IPC: A23K1/10

Abstract: PURPOSE: Provided is a fermenting agent for hygienically producing safe feeds from food wastes, thereby preventing the growth of pathogenic microorganisms, increasing enzyme activity and reduced sugar level and maximizing coefficient of utilization of food wastes. CONSTITUTION: The fermenting agent for hygienically producing safe feeds from food wastes is characteristically composed of 5-15 wt.% of microbial powder and 85-95 wt.% of excipient. It further contains 5-15 part by weight of oligosaccharide, referred to 100 part by weight of the fermenting agent. Wherein the microbial powder is prepared by mixing at least one selected from the group consisting of Bacillus circulans(ATCC 9500), Bacillus megaterium(ATCC 10778), Lactobacillus amylophilus(ATCC 49845), Weissella confusa(ATCC 14434), Bifidus sp.(ATCC 11863), Candida utilis(ATCC 9950) and Candida sp.(ATCC 90238); the excipient is at least one selected from the group consisting of corn flour, rice bran and wheat bran; the oligosaccharide is at least one selected from the group consisting of glucomannan-oligosaccharide, inulo-oligosaccharide, fructo-oligosaccharide and mannan-oligosaccharide.

Abstract translation: 发明目的：提供一种用于卫生生产食品废物安全饲料的发酵剂，由此防止病原微生物的生长，增加酶活性和降低糖水平，并使食品废物的利用系数最大化。 组成：用于从食品垃圾卫生生产安全饲料的发酵剂特征性地由5-15重量％的微生物粉末和85-95重量％的赋形剂组成。 它还含有5-15重量份的低聚糖，指的是100重量份的发酵剂。 其中所述微生物粉末通过混合至少一种选自环状芽孢杆菌（ATCC 9500），巨大芽孢杆菌（ATCC 10778），amylophilus乳杆菌（ATCC 49845），魏斯氏银花（ATCC 14434），双歧菌组成的组制备的。（ATCC 11863），产朊假丝酵母（ATCC 9950）和假丝酵母属（ATCC 90238）; 赋形剂是选自玉米粉，米糠和麦麸中的至少一种; 所述寡糖为选自葡甘露聚糖寡糖，低聚谷氨酸，低聚果糖和甘露寡糖中的至少一种。

公开(公告)号：KR1020030004864A

公开(公告)日：2003-01-15

申请号：KR1020010040594

申请日：2001-07-07

Applicant: 대한민국(농촌진흥청장)

Inventor： 이성실 ,  신기준 ,  정일병 ,  이병석 ,  김창현 ,  정완태 ,  이왕식

IPC: C12N1/00

Abstract: PURPOSE: Provided are a selective culture medium composition for pure acido-halophilic microorganisms viable in semi-fluid conditions and a manufacturing method the selection medium using the same composition, thereby culturing the microorganisms with higher growth rate than in an MRS medium or a YM medium. CONSTITUTION: The selective culture medium composition for pure acido-halophilic microorganisms viable in semi-fluid conditions comprises 3 to 7 g/l of yeast extract, 1.5 to 4 g/l of malt extract, 1.5 to 4 g/l of meat extract, 8 to 12 g/l of tryptone or peptone, 15 to 25 g/l of glucose or lactose, 0.5 to 1.5 g/l of polysorbate 80, 1 to 3 g/l of citric acid or malic acid, 25 to 35 g/l of sodium chloride, and 7 to 9 g/l of agar, wherein 25 to 35 ml/l of glycerol is optionally added to the composition. The method for producing the selective culture medium comprises the steps of: mixing yeast extract, malt extract, meat extract, tryptone or peptone, glucose or lactose, polysorbate 80, citric acid or malic acid, sodium chloride, and agar and inserting the mixture into a flask; adding water into the mixture; slightly heating the mixture; adding glycerol into the heated mixture; and sealing the flask and sterilizing the mixture.

Abstract translation: 目的：提供一种在半流体条件下可行的纯酸性嗜酸性微生物的选择性培养基组合物，以及使用相同组成的选择培养基的制造方法，从而培养生长速度高于MRS培养基或YM培养基的微生物 。 构成：在半流体条件下存在的纯酸性嗜酸性微生物的选择性培养基组合物包含3至7g / l酵母提取物，1.5至4g / l麦芽提取物，1.5至4g / l肉提取物， 8至12g / l胰蛋白胨或蛋白胨，15至25g / l葡萄糖或乳糖，0.5至1.5g / l聚山梨醇酯80,1至3g / l柠檬酸或苹果酸，25至35g / l的氯化钠和7至9g / l的琼脂，其中25至35ml / l的甘油任选加入到组合物中。 制备选择性培养基的方法包括以下步骤：混合酵母提取物，麦芽提取物，肉提取物，胰蛋白胨或蛋白胨，葡萄糖或乳糖，聚山梨酯80，柠檬酸或苹果酸，氯化钠和琼脂，并将混合物 一个烧瓶 向混合物中加入水; 稍微加热混合物; 将甘油加入加热的混合物中; 并密封烧瓶并对混合物进行灭菌。