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公开(公告)号：KR101704123B1
公开(公告)日：2017-02-20
申请号：KR1020140136554
申请日：2014-10-10
Applicant: 대한민국(농촌진흥청장) , 주식회사 청진바이오텍
Abstract: 본발명은 i) 정제봉독을멸균증류수에용해시키는단계; ii) 상기용해된정제봉독을원심분리하여 3kDa 이상의단백질을분리하는단계; iii) 상기원심분리후 분리된정제봉독하층액을동결건조하는단계; iv) 액체크로마토그래피를이용하여상기동결건조된봉독에서멜리틴, 아파민, 포스포리파아제 A2 및 MCD 펩타이드를분리정제하고남은분획물을획득하는단계; 및 v) 상기남은분획물을동결건조하는단계;를포함하는단백질및 펩타이드가제거된것을특징으로하는봉독의분리방법에관한것이다. 또한본 발명은상기방법으로분리된단백질및 펩타이드가제거된봉독및 이를포함하는식품조성물에관한것이다.
Abstract translation: 本发明涉及不含蛋白质和肽的蜂毒提取方法，包括以下步骤：i）将精制蜂毒液溶解于无菌蒸馏水中; ii）通过对溶解的蜂毒进行离心分离过程来提取至少3kDa的蛋白质; iii）进行离心分离处理后的冻干蜂毒丸; iv）使用液相色谱法从冷冻干燥的蜂毒中提取和提炼蜂毒素，阿帕胺，磷脂酶A2和MCD肽，并获得剩余部分; 和v）冷冻干燥剩余部分。 此外，本发明涉及通过该方法除去蛋白质和肽的蜂毒素和包含蜂毒的食物组合物。
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公开(公告)号：KR100814680B1
公开(公告)日：2008-03-20
申请号：KR1020060124927
申请日：2006-12-08
Applicant: 대한민국(농촌진흥청장)
Abstract: A portable apparatus for analyzing nutrient solution is provided to measure concentrations of necessary ions contained in a culture solution used for water culture on the sport rapidly and accurately. A portable apparatus for analyzing nutrient solution(10) comprises: a solid-type ion sensor module(50) measuring concentration of ions; a high resistance input portion(40) which is connected to the solid-type ion sensor module and converts a current signal into an analog voltage signal; an analog-digital converter(30) which is connected to the high resistance input portion and converts the analog voltage signal outputted from the high resistance input portion into a digital signal; and a portable personal digital assistance(PDA)(20) which is connected to the analog-digital converter to display the measured concentration of the ion. The solid-type ion sensor module includes at least two ion sensors selected from the group consisting of K^+ ion sensor, Ca^2+ ion sensor, NH4^+ ion sensor, NO3^- ion sensor, and H^+ ion sensor, each of the ion sensor being ion selective electrode sensor using valinomycin for the K^+ ion electrode, ETH1001 for the Ca^2+ ion electrode, nonactin for the NH4^+, tridodecyl amine for the H^+ ion electrode, and tridodecylmethylammonium nitrate for the NO3^- electrode as an ion selective material, and comprises a substrate, an electrode formed on the substrate, an insulation layer coating at least one portion of the electrode, and an ion selective electrode membrane formed on an exposed portion of the electrode and having a supporting body such as PVC polymer, a plasticizer, and the ion selective material and a lipophilic additive.
Abstract translation: 提供用于分析营养液的便携式装置，用于快速，准确地测量用于水培养的培养液中所含有的必需离子的浓度。 用于分析营养液（10）的便携式设备包括：测量离子浓度的固体离子传感器模块（50）; 连接到固体离子传感器模块并将电流信号转换为模拟电压信号的高电阻输入部分（40）; 模拟数字转换器（30），其连接到高电阻输入部分，并将从高电阻输入部分输出的模拟电压信号转换为数字信号; 以及连接到模拟数字转换器以便显示所测量的离子浓度的便携式个人数字助理（PDA）（20）。 固体离子传感器模块包括至少两个离子传感器，其选自K ^ +离子传感器，Ca 2+离子传感器，NH 4+离子传感器，NO 3离子传感器和H +离子传感器 ，每个离子传感器是使用缬氨霉素用于K 2+离子电极的离子选择性电极传感器，用于Ca 2+离子电极的ETH1001，用于NH 4+的非激活剂，用于H +离子电极的十三烷基胺，以及十三烷基甲基铵 硝酸盐作为离子选择性材料，并且包括基板，形成在基板上的电极，涂覆至少一部分电极的绝缘层和形成在电极的暴露部分上的离子选择性电极膜 电极，并具有诸如PVC聚合物，增塑剂和离子选择性材料的支撑体和亲脂性添加剂。
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公开(公告)号：KR1020160043167A
公开(公告)日：2016-04-21
申请号：KR1020140136554
申请日：2014-10-10
Applicant: 대한민국(농촌진흥청장) , 주식회사 청진바이오텍
Abstract: 본발명은 i) 정제봉독을멸균증류수에용해시키는단계; ii) 상기용해된정제봉독을원심분리하여 3kDa 이상의단백질을분리하는단계; iii) 상기원심분리후 분리된정제봉독하층액을동결건조하는단계; iv) 액체크로마토그래피를이용하여상기동결건조된봉독에서멜리틴, 아파민, 포스포리파아제 A2 및 MCD 펩타이드를분리정제하고남은분획물을획득하는단계; 및 v) 상기남은분획물을동결건조하는단계;를포함하는단백질및 펩타이드가제거된것을특징으로하는봉독의분리방법에관한것이다. 또한본 발명은상기방법으로분리된단백질및 펩타이드가제거된봉독및 이를포함하는식품조성물에관한것이다.
Abstract translation: 本发明涉及不含蛋白质和肽的蜂毒提取方法，包括以下步骤：i）将精制蜂毒液溶解于无菌蒸馏水中; ii）通过对溶解的蜂毒进行离心分离过程来提取至少3kDa的蛋白质; iii）进行离心分离处理后的冻干蜂毒丸; iv）使用液相色谱法从冷冻干燥的蜂毒中提取和提炼蜂毒素，阿帕胺，磷脂酶A2和MCD肽，并获得剩余部分; 和v）冷冻干燥剩余部分。 此外，本发明涉及通过该方法除去蛋白质和肽的蜂毒素和包含蜂毒的食物组合物。
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