公开(公告)号：KR1020080043164A

公开(公告)日：2008-05-16

申请号：KR1020060111866

申请日：2006-11-13

Applicant: 대한민국(농촌진흥청장)

Inventor： 조광수 ,  홍수영 ,  유승열 ,  우종규 ,  문지영 ,  장석우

IPC: C12N15/09 ,  C12N15/00 ,  C12Q1/68

CPC classification number: C12N15/09 ,  C12N15/00 ,  C12Q1/6844 ,  C12Q2535/139

Abstract: A method for identification of a clubroot resistance gene in Chinese cabbage is provided to reduce the identification time by performing identification with RAPD(randomly amplified polymorphic DNA) marker in a laboratory without a resistance examination process in the field, and screen a clubroot-resistant individual rapidly in a relatively small space. A clubroot resistance gene in Chinese cabbage is identified by confirming the genotype of Chinese cabbage clubroot resistance with RAPD marker, and selecting a resistant individual "CR-serona", wherein the resistance gene analysis of "CR-serona" is performed by discerning the resistance gene pattern of Chinese cabbage clubroot by using single spore-derived pathogenic bacteria(Rrace 4, Willinams method) and a group of F2, BC1P1 and BC1P2, and extracting DNAs from resistant and susceptible individuals from 230 individuals of F2 group and developing DNA marker closely associated with the resistance gene by BSA-RAPD(bulked segregant analysis-randomly amplified polymorphic DNA); and the RAPD marker is a DNA fragment amplified specifically by PCR(polymerase chain reaction) of Chinese cabbage genome by using RAPD primer(OPJ05).

Abstract translation: 提供了一种用于鉴定大白菜的根茎抗性基因的方法，通过在实地没有电阻检查过程的RAPD（随机扩增多态性DNA）标记物进行鉴定来减少识别时间，并筛选出一个耐发丝的个体 迅速在相对较小的空间。 通过使用RAPD标记确认大白菜丝状杆菌耐药性的基因型，选择抗性个体“CR-serona”来鉴定大白菜中的种子抗性基因，其中通过鉴别抗性来进行“CR-serona”的抗性基因分析 通过使用单孢子源性病原菌（Rrace 4，Willinams法）和一组F2，BC1P1和BC1P2，从F2群体230个个体的抗性和易感个体提取DNA，并开发DNA标记 与BSA-RAPD的抗性基因相关（大量分离分析 - 随机扩增多态性DNA）; RAPD标记是通过使用RAPD引物（OPJ05）的大白菜基因组的PCR（聚合酶链式反应）特异性扩增的DNA片段。