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公开(公告)号：KR101642775B1
公开(公告)日：2016-07-28
申请号：KR1020140164268
申请日：2014-11-24
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/68
Abstract: 본발명은감자글리코알칼로이드저함유계통을선발하기위한프라이머및 이를이용한감자글리코알칼로이드저함유품종선발방법에관한것이다. 보다상세하게, 본발명은건조스트레스처리시 감자글리코알칼로이드함량과정의상관이있는생합성유전자의발현수준을검출하기위한프라이머및 qRT-PCR(quantitative real time PCR) 방법에관한것이다. 또한상기생합성유전자의발현수준을선발지표로활용하여인체에독성을유발하는글리코알칼로이드저함유감자품종을선발하는방법에관한것이다.
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公开(公告)号：KR1020080043164A
公开(公告)日：2008-05-16
申请号：KR1020060111866
申请日：2006-11-13
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/09 , C12N15/00 , C12Q1/6844 , C12Q2535/139
Abstract: A method for identification of a clubroot resistance gene in Chinese cabbage is provided to reduce the identification time by performing identification with RAPD(randomly amplified polymorphic DNA) marker in a laboratory without a resistance examination process in the field, and screen a clubroot-resistant individual rapidly in a relatively small space. A clubroot resistance gene in Chinese cabbage is identified by confirming the genotype of Chinese cabbage clubroot resistance with RAPD marker, and selecting a resistant individual "CR-serona", wherein the resistance gene analysis of "CR-serona" is performed by discerning the resistance gene pattern of Chinese cabbage clubroot by using single spore-derived pathogenic bacteria(Rrace 4, Willinams method) and a group of F2, BC1P1 and BC1P2, and extracting DNAs from resistant and susceptible individuals from 230 individuals of F2 group and developing DNA marker closely associated with the resistance gene by BSA-RAPD(bulked segregant analysis-randomly amplified polymorphic DNA); and the RAPD marker is a DNA fragment amplified specifically by PCR(polymerase chain reaction) of Chinese cabbage genome by using RAPD primer(OPJ05).
Abstract translation: 提供了一种用于鉴定大白菜的根茎抗性基因的方法，通过在实地没有电阻检查过程的RAPD（随机扩增多态性DNA）标记物进行鉴定来减少识别时间，并筛选出一个耐发丝的个体 迅速在相对较小的空间。 通过使用RAPD标记确认大白菜丝状杆菌耐药性的基因型，选择抗性个体“CR-serona”来鉴定大白菜中的种子抗性基因，其中通过鉴别抗性来进行“CR-serona”的抗性基因分析 通过使用单孢子源性病原菌（Rrace 4，Willinams法）和一组F2，BC1P1和BC1P2，从F2群体230个个体的抗性和易感个体提取DNA，并开发DNA标记 与BSA-RAPD的抗性基因相关（大量分离分析 - 随机扩增多态性DNA）; RAPD标记是通过使用RAPD引物（OPJ05）的大白菜基因组的PCR（聚合酶链式反应）特异性扩增的DNA片段。
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公开(公告)号：KR1020030000404A
公开(公告)日：2003-01-06
申请号：KR1020010036169
申请日：2001-06-25
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/68
Abstract: PURPOSE: An identification method for a cytoplasmic male sterility factor using PCR-RFLP in onion is provided, to give high creditability and accuracy for test results, to significantly decrease time for identification from 4-8 years to one week and to increase breeding efficiency without involving no test cross and post sterility clarification. CONSTITUTION: The identification method for a cytoplasmic male sterility factor using PCR-RFLP in onion comprises the steps of: amplifying psbA gene of onion by using PCR; and identifying cytoplasmic male sterile genes of onion by RFLP analysis, wherein the identification process is carried out by digesting PCR products of the psbA gene of onion with MspI, and identifying the psbA gene of onion as an N-type gene when it is digested, or as an S-type gene when it is not digested.
Abstract translation: 目的：提供在洋葱中使用PCR-RFLP的细胞质雄性不育因子的鉴定方法，为测试结果提供高可信度和准确性，大大缩短了鉴定时间，从4-8年到一周，并提高了育种效率，而没有 不包括测试交叉和后不育澄清。 构成：在洋葱中使用PCR-RFLP的细胞质雄性不育因子的鉴定方法包括以下步骤：通过PCR扩增洋葱的psbA基因; 并通过RFLP分析鉴定洋葱的细胞质雄性不育基因，其中鉴定过程是通过用MspI消化洋葱psbA基因的PCR产物进行的，并且当消化时鉴定洋葱的psbA基因作为N型基因， 或当不消化时作为S型基因。
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公开(公告)号：KR1020170010226A
公开(公告)日：2017-01-26
申请号：KR1020150101243
申请日：2015-07-16
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/68
Abstract: 본발명은감자의근연야생종과재배종을구별하기위한마커에관한것이다. 또한본 발명은감자의근연야생종과재배종을구별하기위한프라이머세트를제공하는것이다. 또한본 발명은대상시료에서게놈 DNA를분리하는단계; 상기분리된게놈 DNA를주형으로하고, 상기프라이머세트를이용하여 PCR(polymerase chain reaction)을수행하여표적서열을증폭하는단계; 및상기증폭산물을동정하는단계;를포함하는, 감자의근연야생종과재배종을판별하는방법에관한것이다. 또한본 발명은감자의근연야생종과재배종감자의체세포융합체후대세포질선발방법에관한것이다.
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Patent Agency Ranking
公开(公告)号：KR100435152B1
公开(公告)日：2004-06-14
申请号：KR1020010036169
申请日：2001-06-25
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/68
Abstract: PURPOSE: An identification method for a cytoplasmic male sterility factor using PCR-RFLP in onion is provided, to give high creditability and accuracy for test results, to significantly decrease time for identification from 4-8 years to one week and to increase breeding efficiency without involving no test cross and post sterility clarification. CONSTITUTION: The identification method for a cytoplasmic male sterility factor using PCR-RFLP in onion comprises the steps of: amplifying psbA gene of onion by using PCR; and identifying cytoplasmic male sterile genes of onion by RFLP analysis, wherein the identification process is carried out by digesting PCR products of the psbA gene of onion with MspI, and identifying the psbA gene of onion as an N-type gene when it is digested, or as an S-type gene when it is not digested.
Abstract translation: 目的：提供一种在洋葱中使用PCR-RFLP的细胞质雄性不育因子的鉴定方法，以提高测试结果的可信性和准确性，显着缩短鉴定时间从4-8年到一周并提高育种效率，而无需 不涉及测试交叉和无菌后澄清。 构成：在洋葱中使用PCR-RFLP鉴定细胞质雄性不育因子的方法包括以下步骤：用PCR扩增洋葱的psbA基因; 并通过RFLP分析鉴定洋葱的细胞质雄性不育基因，其中鉴定过程是通过用MspI消化洋葱psbA基因的PCR产物，并在消化时将洋葱的psbA基因鉴定为N型基因来进行的， 或者当它不被消化时作为S型基因。
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公开(公告)号：KR101740571B1
公开(公告)日：2017-05-30
申请号：KR1020150015154
申请日：2015-01-30
Applicant: 대한민국(농촌진흥청장)
CPC classification number: Y02A40/17 , Y02P60/146 , Y02P60/216
Abstract: 본발명은씨감자생산용묘를옮겨심을수 있는적어도하나의씨감자묘정식구가형성된정식패널; 상기정식패널이안치되는공간이형성되고, 상기정식패널에정식된씨감자묘의생장에필요한배양액을담액하는베드; 상기베드내부로배양액을순환시키기위한순환펌프와연결되어상기베드에상기배양액을공급하는공급관; 및상기베드를순환하여배출되는배양액을회수하는퇴수관; 을포함하며, 상기퇴수관에는상기베드내에배양액의수위를유지하도록베드의하단으로부터소정간격이격되는배수구가형성되어, 상기정식된씨감자묘는소정부위상기배양액에담액되는것을특징으로하는순환식담액수경장치에관한것이다.
Abstract translation: 本发明涉及一种正式小组，其中至少一个种子马铃薯家族成员被种植以转移植物种子马铃薯; 的空间已满面板被放置在床上，为生长到完全正式面板种薯幼苗所需要的介质damaek形成; 连接到循环泵的供给管，用于使培养液循环到床中以将培养液供给到床; 以及用于收集通过床循环排出的培养液的排水管; 包括toesu管循环damaek其特征在于，形成有在预定距离离床，以保持在床内的培养液的水平的底部的排放，damaek充分种薯墓预定部分的培养 到水培设备。
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公开(公告)号：KR101728893B1
公开(公告)日：2017-04-28
申请号：KR1020150101243
申请日：2015-07-16
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/68
Abstract: 본발명은감자의근연야생종과재배종을구별하기위한마커에관한것이다. 또한본 발명은감자의근연야생종과재배종을구별하기위한프라이머세트를제공하는것이다. 또한본 발명은대상시료에서게놈 DNA를분리하는단계; 상기분리된게놈 DNA를주형으로하고, 상기프라이머세트를이용하여 PCR(polymerase chain reaction)을수행하여표적서열을증폭하는단계; 및상기증폭산물을동정하는단계;를포함하는, 감자의근연야생종과재배종을판별하는방법에관한것이다. 또한본 발명은감자의근연야생종과재배종감자의체세포융합체후대세포질선발방법에관한것이다.
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