公开(公告)号：KR100404767B1

公开(公告)日：2003-11-07

申请号：KR1020010016649

申请日：2001-03-29

Applicant: 대한민국(농촌진흥청장) ,  윤철식

Inventor： 윤철식 ,  이민호 ,  김홍선 ,  유재기 ,  김정준 ,  조점래 ,  최병렬

IPC: C12N1/20

Abstract: PURPOSE: A microorganism Verticillium lecanii CS626 and a method for the control of harmful insects using the same microorganism are provided, thereby effectively preventing the growth of the harmful insects living in agricultural equipments. CONSTITUTION: The microorganism Verticillium lecanii CS626(KFCC-11277) has an improved insecticidal effect on the harmful insects living in agricultural equipments, wherein it is isolated by collecting a mycelium from the surface of an insect infected by Verticillium lecanni; inoculating the mycelium on a potato agar medium containing tetracycline; culturing it at 22 to 28 deg. C; isolating spores and mycelia from the cultured medium; suspending the isolated spores in 0.01% tween 80 solution; spraying the suspension on a leaf containing insects; and selecting a microorganism showing excellent insecticidal activity.

Abstract translation: 目的：提供一种微生物蜡蚧轮枝菌（Verticillium lecanii）CS626和使用相同微生物控制有害昆虫的方法，从而有效地防止住在农业设备中的有害昆虫的生长。 组成：微生物蜡蚧轮枝菌Verticillium lecanii CS626（KFCC-11277）对农业设备中的有害昆虫具有改善的杀虫效果，其中通过从被轮枝孢菌感染的昆虫表面收集菌丝体来分离它; 将菌丝体接种在含有四环素的马铃薯琼脂培养基上; 在22至28度培养它。 C; 从培养基中分离孢子和菌丝体; 将分离的孢子悬浮在0.01％吐温80溶液中; 将悬浮液喷洒在含有昆虫的叶上; 并选择显示优异的杀虫活性的微生物。

公开(公告)号：KR100239209B1

公开(公告)日：2000-01-15

申请号：KR1019970051035

申请日：1997-10-02

Applicant: 대한민국(농촌진흥청장)

Inventor： 이정운 ,  윤철식 ,  이영욱 ,  이상계 ,  유재기 ,  문기혁 ,  성기호

IPC: C12N1/20

Abstract: 본 발명은 십자화과 배추에 심각한 피해를 유발하는 배추좀나방에 대하여 우수한 살충력을 갖는 백강균(Beauveria bassiana) CS-1 균주(KFCC-10988) 및 이를 이용하여 배추좀나방을 방제하는 방법에 관한 것이다.

公开(公告)号：KR100426462B1

公开(公告)日：2004-04-17

申请号：KR1020010007901

申请日：2001-02-16

Applicant: 대한민국(농촌진흥청장)

Inventor： 김정환 ,  김용헌 ,  고현관 ,  한만위 ,  유재기

IPC: A01K67/033

Abstract: PURPOSE: A method for breeding food of oriusspp and a breeding apparatus thereof are provided to more safely and effectively supply a large amount of cadra cautella instead of plant louse or mite. CONSTITUTION: The method for breeding food of oriusspp and a breeding apparatus thereof is comprised of steps of; uniformly covering the bottom of a larvae breeding container with feed, and spreading eggs of cadra cautella; extracting a lump of mixed pupae and feed when the eggs become pupae within the breeding container maintaining a temperature of 25deg.C, and fixing the lump with a pupae protecting member with interposing it between iron nets(5,5'); storing the iron nets on the upper end of a light permissible imago eclosion frame(10) with loops; and collecting eggs dropped from the imagoes in a box(40).

Abstract translation: 目的：提供一种用于培养oriusspp食物的方法及其培育装置，以更安全有效地供应大量的cadra cautella而不是植物虱或螨。 组成：oriusspp食品的饲养方法及其饲养设备包括以下步骤： 用饲料均匀地覆盖幼虫繁殖容器的底部，铺上小菜蛾的卵; 提取一块混合的蛹并在饲养温度为25℃时在蛹中成为蛹时进行饲喂，并用蛹保护件将块固定在铁网（5,5'）之间; 将铁网存放在具有环的轻的许可的成象羽化框架（10）的上端; 并收集从成像中投下的蛋（40）。

公开(公告)号：KR1020020076646A

公开(公告)日：2002-10-11

申请号：KR1020010016649

申请日：2001-03-29

Applicant: 대한민국(농촌진흥청장) ,  윤철식

Inventor： 윤철식 ,  이민호 ,  김홍선 ,  유재기 ,  김정준 ,  조점래 ,  최병렬

IPC: C12N1/20

Abstract: PURPOSE: A microorganism Verticillium lecanii CS626 and a method for the control of harmful insects using the same microorganism are provided, thereby effectively preventing the growth of the harmful insects living in agricultural equipments. CONSTITUTION: The microorganism Verticillium lecanii CS626(KFCC-11277) has an improved insecticidal effect on the harmful insects living in agricultural equipments, wherein it is isolated by collecting a mycelium from the surface of an insect infected by Verticillium lecanni; inoculating the mycelium on a potato agar medium containing tetracycline; culturing it at 22 to 28 deg. C; isolating spores and mycelia from the cultured medium; suspending the isolated spores in 0.01% tween 80 solution; spraying the suspension on a leaf containing insects; and selecting a microorganism showing excellent insecticidal activity.

Abstract translation: 目的：提供微生物轮枝孢菌CS626和使用相同微生物控制害虫的方法，从而有效防止生活在农业设备中的有害昆虫的生长。 构成：微生物轮枝孢属植物CS626（KFCC-11277）对农业设备中的有害昆虫具有改善的杀虫作用，其通过从轮枝堇病毒感染的昆虫表面收集菌丝来分离， 将菌丝体接种在含有四环素的马铃薯琼脂培养基上; 在22〜28度培养 C; 从培养基中分离孢子和菌丝体; 将分离的孢子悬浮在0.01％吐温80溶液中; 将悬浮液喷洒在含有昆虫的叶上; 并选择显示出优异的杀虫活性的微生物。

公开(公告)号：KR1020020067369A

公开(公告)日：2002-08-22

申请号：KR1020010007901

申请日：2001-02-16

Applicant: 대한민국(농촌진흥청장)

Inventor： 김정환 ,  김용헌 ,  고현관 ,  한만위 ,  유재기

IPC: A01K67/033

CPC classification number: A01K67/033

Abstract: PURPOSE: A method for breeding food of oriusspp and a breeding apparatus thereof are provided to more safely and effectively supply a large amount of cadra cautella instead of plant louse or mite. CONSTITUTION: The method for breeding food of oriusspp and a breeding apparatus thereof is comprised of steps of; uniformly covering the bottom of a larvae breeding container with feed, and spreading eggs of cadra cautella; extracting a lump of mixed pupae and feed when the eggs become pupae within the breeding container maintaining a temperature of 25deg.C, and fixing the lump with a pupae protecting member with interposing it between iron nets(5,5'); storing the iron nets on the upper end of a light permissible imago eclosion frame(10) with loops; and collecting eggs dropped from the imagoes in a box(40).

Abstract translation: 目的：提供一种用于育种oriusspp食品的方法及其育种装置，以更安全有效地供应大量的睾丸而不是植物虱子或螨虫。 构成：用于育种oriusspp食品的方法及其育种装置包括以下步骤： 用饲料均匀覆盖幼虫繁殖容器的底部，并传播蛹虫卵; 当饲养容器内的卵成为蛹时保持温度为25℃，并用蛹保护部件将块固定在铁网（5,5'）之间，从而提取一团混合蛹。 将铁网存放在具有环的光允许成像框架（10）的上端; 并从一个盒子里的成像中收集蛋（40）。

公开(公告)号：KR1019990030698A

公开(公告)日：1999-05-06

申请号：KR1019970051035

申请日：1997-10-02

Applicant: 대한민국(농촌진흥청장)

Inventor： 이정운 ,  윤철식 ,  이영욱 ,  이상계 ,  유재기 ,  문기혁 ,  성기호

IPC: C12N1/20

Abstract: 본 발명은 십자화과 배추에 심각한 피해를 유발하는 배추좀나방에 대하여 우수한 살충력을 갖는 백강균(Beauveria bassiana) CS-1 균주(KFCC-10988) 및 이를 이용하여 배추좀나방을 방제하는 방법에 관한 것이다.

公开(公告)号：KR100475346B1

公开(公告)日：2005-03-10

申请号：KR1020020040749

申请日：2002-07-12

Applicant: 대한민국(농촌진흥청장) ,  학교법인고려중앙학원

Inventor： 김정준 ,  유재기 ,  이민호 ,  김홍선 ,  조점래

IPC: C12N1/14

Abstract: 본 발명은 국내 토착 균주
버티실리움 레카니 CS625(
Verticillium lecanii CS625) 및 이를 이용한 진딧물 방제 방법에 관한 것이다. 보다 구체적으로는, 진딧물에 대하여 살충력이 우수하고 시설 재배지 환경조건에서도 잘 적응된 국내 토착 균주
버티실리움 레카니 CS625 균주, 상기 균주 및 균주의 포자 현탁액을 함유하는 진딧물 방제용 조성물 및 상기 조성물을 농작물에 살포하여 진딧물, 특히 목화진딧물 및 복숭아혹진딧물을 생물학적으로 방제하는 방법에 관한 것이다. 본 발명 균주는 복숭아혹진딧물로부터 분리한 국내 토착 균주로, 시설 재배지의 환경조건에서도 생장률이 우수할 뿐만 아니라, 상기 균주 및 균주로부터 생성된 포자현탁액을 시설 재배재의 농작물에 처리 시 목화진딧물을 효과적으로 방제할 수 있는 뛰어난 효과가 있다.

公开(公告)号：KR1020040006457A

公开(公告)日：2004-01-24

申请号：KR1020020040749

申请日：2002-07-12

Applicant: 대한민국(농촌진흥청장) ,  학교법인고려중앙학원

Inventor： 김정준 ,  유재기 ,  이민호 ,  김홍선 ,  조점래

IPC: C12N1/14

Abstract: PURPOSE: A microorganism Verticillium lecanii CS625 and a method for the control of aphids using the same are provided. The microorganism has improved growth rate in protected cultivation and effectively controls aphids in crops being grown by the protected cultivation. CONSTITUTION: A microorganism Verticillium lecanii CS625(KACC 93002) is provided. A composition for the control of aphids comprises the spore suspension produced from Verticillium lecanii CS625(KACC 93002) as an effective component, wherein the spore suspension comprises conidium or blastopore produced from Verticillium lecanii CS625(KACC 93002); and the spore suspension contains 10(6) to 10(10) cfu/ml of spores. A method for the control of aphids comprises spraying the aphid controlling composition to crops.

Abstract translation: 目的：提供微生物轮枝孢菌CS625和使用其的蚜虫控制方法。 微生物在受保护的栽培中具有改善的生长速度，并有效控制受保护栽培种植的作物中的蚜虫。 构成：提供了一种微生物轮枝孢菌TC625（KACC 93002）。 用于控制蚜虫的组合物包含作为有效成分的由轮枝孢霉属植物轮枝孢菌CS625（KACC 93002）产生的孢子悬浮液，其中孢子悬浮液包含由轮枝孢虱CS625（KACC 93002）产生的分生孢子或孢子虫; 孢子悬浮液含有10（6）至10（10）cfu / ml孢子。 用于控制蚜虫的方法包括将蚜虫控制组合物喷洒到作物上。