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    장미 스위트엘로우 체세포배(배발생캘러스 포함)를 이용한 형질전환 식물체 대량획득방법
    2.
    发明公开
    장미 스위트엘로우 체세포배(배발생캘러스 포함)를 이용한 형질전환 식물체 대량획득방법 有权
    通过使用SOMATIC EMBRYO（诱发香草蜂蜜酱）在玫瑰黄色中发酵转基因植物的大量生产方法

    公开(公告)号：KR1020120043371A

    公开(公告)日：2012-05-04

    申请号：KR1020100104647

    申请日：2010-10-26

    Applicant: 대한민국(농촌진흥청장)

    Inventor： 이수영 ,  김성태 ,  이은경 ,  김원희 ,  이정림 ,  한봉희

    IPC: A01H5/00 ,  C12N15/87 ,  C12N5/04 ,  C12N5/02

    CPC classification number: C12N15/8205 ,  A01H4/001 ,  A01H4/005 ,  C12N5/0025 ,  C12N2500/34 ,  C12N2501/30

    Abstract: PURPOSE: A method for producing a large amount of transgenic plants using somatic cell embryo of Sweet Yellow is provided. CONSTITUTION: A method for producing a large amount of transgenic plants using rose 'Sweet Yellow' somatic cell embryo and embryogenic callus comprises: a step of co-culturing somatic cell embryo GUS gene-transformed Agrobacterium tumefaciens and GFP gene-transformed Agrobacterium tumefaciens; a step of culturing the co-cultured somatic cell embryo or embryogenic callues in an embryo selection medium containing 250mg/L^-1 of cefotaxim; a step of culturing the somatic cell embryo or embryogenic callus in a medium containing cefotaxim solely or together with PPT(phosphinothricin); a step of culturing a cultured shoot in a medium; a step of culturing the shoot in a shoot growth medium; a step of culturing 1-1.5cm of shoots in a multi-shoot growth medium; and a step of culturing the multi-shoots in a root inducing medium.

    Abstract translation: 目的：提供使用甜黄体细胞胚的大量转基因植物的生产方法。 构成：使用玫瑰“甜黄”体细胞胚和胚发生愈伤组织生产大量转基因植物的方法包括：共培养体细胞胚GUS基因转化的根癌土壤杆菌和GFP基因转化的根癌土壤杆菌的步骤; 在含有250mg / L ^ 1的头孢噻肟的胚胎选择培养基中培养共培养的体细胞胚胎或胚胎发育的洗脱液的步骤; 在含有头孢噻肟的培养基中单独或与PPT（次膦酸）一起培养体细胞胚或胚发生愈伤组织的步骤; 在培养基中培养培养的苗的步骤; 在芽生长培养基中培养芽的步骤; 在多枝生长培养基中培养1-1.5cm的芽的步骤; 以及在根诱导培养基中培养多芽的步骤。

    대기환경오염 저항성 페튜니아 형질전환체
    3.
    发明公开
    대기환경오염 저항성 페튜니아 형질전환체 有权
    改变了对空气污染的耐宠物

    公开(公告)号：KR1020130056546A

    公开(公告)日：2013-05-30

    申请号：KR1020110122205

    申请日：2011-11-22

    Applicant: 대한민국(농촌진흥청장)

    Inventor： 이수영 ,  이정림 ,  이영란 ,  김영태 ,  김정호 ,  김성태 ,  이은경 ,  권오현 ,  김원희

    IPC: A01H5/00 ,  C12N15/82 ,  A01H1/02

    CPC classification number: C12N15/8271 ,  A01H1/02 ,  A01H1/04 ,  C12N15/8205 ,  C12N15/8209

    Abstract: PURPOSE: A Petunia transformant containing SOD gene and NDPK2 gene is provided to ensure resistance to air pollution, especially sulphur dioxide. CONSTITUTION: A method for preparing a Petunia transformant with resistance to air pollution comprises: a step of introducing SOD gene to Petunia and preparing transformed Petunia in which a gene is stably immobilized through generation advancement; a step of introducing NDPK2 gene to Petunia and preparing transformed Petunia in which a gene is stably immobilized through generation advancement; a step of crossbreeding the transformants to introduce SOD gene and NDPK2 gene; a step of selecting a transformant containing SOD gene and NDPK2 gene with resistance to sulphur dioxide; and a step of self-pollination of the transformant and secondarily selecting a sulphur dioxide with resistance to sulphur dioxide. [Reference numerals] (A) Treat with 60ppm of sulfurous acid gas for 8 hours; (AA,CC) 2 days after treatment; (B) Treat with 60ppm of sulfurous acid gas for 4 hours; (BB,DD) 7 days after treatment

    Abstract translation: 目的：提供含有SOD基因和NDPK2基因的矮牵牛转化子，以确保抵抗空气污染，特别是二氧化硫。 构成：制备抗空气污染的矮牵牛转化体的方法包括：向矮牵牛引入SOD基因并通过产生提前稳定地固定基因的转化矮牵牛的步骤; 将NDPK2基因导入矮牵牛的步骤，并通过发育前进制备转基因小麦，其中基因被稳定地固定; 使转化子杂交引入SOD基因和NDPK2基因的步骤; 选择含有SOD基因和具有二氧化硫抗性的NDPK2基因的转化体的步骤; 以及转化体的自花传粉的步骤，并且二次选择耐二氧化硫的二氧化硫。 （附图标记）（A）用60ppm的亚硫酸气处理8小时; （AA，CC）治疗后2天; （B）用60ppm亚硫酸气处理4小时; （BB，DD）治疗后7天

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