公开(公告)号：KR1020030012998A

公开(公告)日：2003-02-14

申请号：KR1020010047271

申请日：2001-08-06

Applicant: 대한민국(농촌진흥청장)

Inventor： 지상덕 ,  조세연 ,  남성희 ,  송병권 ,  이효순

IPC: A01G1/04

CPC classification number: A61K36/068

Abstract: PURPOSE: A method for culturing Isaria sinclairii is provided by inoculating Isaria sinclairii to the pupa or larva of a domestic silkworm, thereby mass producing Isaria sinclairii . CONSTITUTION: The method for culturing Isaria sinclairii using a domestic silkworm comprises the steps of: inoculating spores of Isaria sinclairii to the larva or pupa of a domestic silkworm by spraying the spores on the surface of the larva or the pupa; and forming a fruit body of Isaria sinclairii from the infected pupa, wherein the inoculation process is carried out by diluting the spores of Isaria sinclairii in water in the concentration of 106/ml to 108/ml, spraying the diluted solution on the surfaces of the larvae or pupae of the domestic silkworms aged 2 weeks, 3 weeks, 4 weeks and 5 weeks, and leaving them under 95% of humidity for 12 hours; the larvae or pupae of the domestic silkworms are stored at 20 deg. C or less prior to the inoculation; and the fruit body formation process is carried out under conditions of 15 to 25 deg. C and humidity of 95%.

Abstract translation: 目的：通过将Isaria sinclairii接种到家蚕的蛹或幼虫来提供Isaria sinclairii的培养方法，从而大量生产Isaria sinclairii。 构成：使用家蚕培养伊蚊（Isaria sinclairii）的方法包括以下步骤：通过将孢子喷洒在幼虫或蛹的表面上，将Isaria sinclairii的孢子接种到家蚕的幼虫或蛹中; 并从感染的蛹中形成Isaria sinclairii的果实体，其中通过在浓度为106 / ml至108 / ml的水中稀释Isaria sinclairii的孢子进行接种过程，将稀释的溶液喷洒在 家蚕的幼虫或蛹2周，3周，4周和5周，并将其置于95％湿度下12小时; 家蚕的幼虫或蛹保存在20度。 C以下; 果实体形成处理在15〜25℃的条件下进行。 C和湿度95％。

公开(公告)号：KR100407072B1

公开(公告)日：2003-11-28

申请号：KR1020010047271

申请日：2001-08-06

Applicant: 대한민국(농촌진흥청장)

Inventor： 지상덕 ,  조세연 ,  남성희 ,  송병권 ,  이효순

IPC: A01G1/04

Abstract: PURPOSE: A method for culturing Isaria sinclairii is provided by inoculating Isaria sinclairii to the pupa or larva of a domestic silkworm, thereby mass producing Isaria sinclairii . CONSTITUTION: The method for culturing Isaria sinclairii using a domestic silkworm comprises the steps of: inoculating spores of Isaria sinclairii to the larva or pupa of a domestic silkworm by spraying the spores on the surface of the larva or the pupa; and forming a fruit body of Isaria sinclairii from the infected pupa, wherein the inoculation process is carried out by diluting the spores of Isaria sinclairii in water in the concentration of 106/ml to 108/ml, spraying the diluted solution on the surfaces of the larvae or pupae of the domestic silkworms aged 2 weeks, 3 weeks, 4 weeks and 5 weeks, and leaving them under 95% of humidity for 12 hours; the larvae or pupae of the domestic silkworms are stored at 20 deg. C or less prior to the inoculation; and the fruit body formation process is carried out under conditions of 15 to 25 deg. C and humidity of 95%.

Abstract translation: 目的：通过将Isaria sinclairii接种到家蚕的蛹或幼虫来提供Isaria sinclairii的培养方法，由此大量生产Isaria sinclairii。 组成：使用家蚕培养Isaria sinclairii的方法包括以下步骤：通过将孢子喷洒在幼虫或蛹表面上，将Isaria sinclairii的孢子接种到家蚕的幼虫或蛹中; 并从感染的蛹中形成Isaria sinclairii的果实体，其中接种过程通过将Isaria sinclairii的孢子以106 / ml的浓度稀释到10 8 / ml，将稀释的溶液喷雾到 2周，3周，4周和5周的家蚕的幼虫或蛹，并将其置于95％的湿度下12小时; 家蚕的幼虫或蛹储存在20℃。 接种前C或更少; 并且在15至25度的条件下进行子实体形成过程。 C和95％的湿度。