Abstract:
PURPOSE: A composition containing a DNA marker for selecting Oryza sativa with green rice leaf hopper-resistant gene Grh1 is provided to efficiently distinguish green rice leaf hopper resistant and sensitive individual. CONSTITUTION: A composition for selecting Oryza sativa species with green rice leaf hopper-resistance contains a primer for detecting the presence of an oligonucleotide or a fragment thereof. The primer is one or more primer sets selected from sequence numbers 1-14. The primer set is one or more primer sets selected from sequence numbers 3-14. A method for selecting the Oryza sativa species comprises: a step of isolating genome DNA from Oryza sativa samples; a step of amplifying a target sequence using the genome DNA as a template and the primer set; and a step of detecting the amplifies product. The sample is derived from roots, leaves, stems, flowers, or buds of Oryza sativa. A kit for selecting Oryza sativa species with green rice leaf hopper-resistance contains the primer set selected from sequence numbers 1-14, restriction enzyme, and reagent.
Abstract:
본 발명은 RM516, RM6082, RM18182, RM18184, RM18185, RM5844 및 RM7409로 구성되는 군으로부터 선택된 하나 이상의 올리고뉴클레오티드 또는 이의 단편의 존재여부를 검출하는 프라이머를 포함하는, 끝동매미충 저항성 벼 품종 선별용 조성물, 구체적으로는, 서열번호 1 내지 서열번호 14로 구성되는 군으로부터 선택되는 하나 이상의 프라이머 세트를 포함하는, 끝동매미충 저항성 벼 품종 선별용 조성물에 관한 것이다. 또한, 본 발명은 벼 시료에서 게놈 DNA를 분리하는 단계; 상기 분리된 게놈 DNA를 주형으로 하고, 서열번호 1 내지 서열번호 14로 구성되는 군으로부터 선택되는 하나 이상의 프라이머 세트를 이용하여 증폭 반응을 수행하여 표적 서열을 증폭하는 단계; 및 상기 증폭 산물을 검출하는 단계를 포함하는, 끝동매미충 저항성 벼 품종 선별 방법 및 벼 선별 키트에 관한 것이다. 본 발명에 따른 끝동매미충 저항성 개체를 선발하는 방법을 이용하면, 끝동매미충 저항성 유전자의 위치와 아주 밀접하게 연관된 마커를 이용하여 효율적으로 끝동매미충 저항성 및 감수성 개체를 구분할 수 있다.
Abstract:
PURPOSE: A producing method of rice paper having soft eating texture is provided to prevent the generation of bad smell from the rice paper by using rice and potato starch in the predetermined ratio. CONSTITUTION: A producing method of rice paper having soft eating texture comprises the following steps: soaking rice in water for 1-24 hours at 20-40 deg C; mixing 100 parts of water soaked rice by weight with 30-70 parts of water by weight, and crushing the mixture before drying to obtain rice powder; mixing the rice powder with potato starch, and adding more water and salt into the mixture to obtain dough; spreading the dough on a fabric, and steaming the dough; firstly drying the steamed product at 30-60 deg C for 30 minutes-2 hours in 60-80% humidity; secondly drying the product at room temperature in 60-80% of relative humidity; and cutting and packaging the dried product. The rice powder is mixed with 10-90wt% of potato starch. [Reference numerals] (AA) Domestic rice; (BB) Polishing and assorting; (CC) Soaking(4 hours); (DD) Wet-milling and drying; (EE) Assorting with a sieve(100meshes); (FF) Rice powder:potato starch (1:1) water(2.25 times)/salt 1%; (GG) Steaming; (HH) First drying, wind(40 deg. C/70%RH); (II) Secondly drying, windless(room temperature/70%RH); (JJ) Cutting and packaging