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公开(公告)号：KR1020180003041A
公开(公告)日：2018-01-09
申请号：KR1020160082336
申请日：2016-06-30
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/70 , G01N33/569
CPC classification number: C12Q1/701 , C12Q2525/205 , G01N33/56983
Abstract: 본발명은보리로부터유래된서열번호 1의염기서열을포함하는신규한바이러스유전자, 상기신규한바이러스유전자또는상기유전자로부터발현된단백질을검출할수 있는제제를포함하는보리의바이러스감염여부진단용조성물, 상기보리의바이러스감염여부진단용조성물을포함하는보리의바이러스감염여부진단용키트및 상기조성물또는키트를이용하여보리의바이러스감염여부를진단하는방법에관한것이다. 본발명에서제공하는보리의바이러스감염여부진단용조성물또는키트를사용하면, 지금까지알려지지않았던신규한바이러스에의한보리의감염여부를진단할수 있으므로, 바이러스감염에의한보리의질병발생여부의판단에널리활용될수 있을것이다.
Abstract translation: 本发明涉及新的病毒基因，含有制剂大麦的新型病毒基因或病毒感染，可以检测从基因表达的蛋白质诊断组合物是否包含SEQ ID NO：1的核苷酸序列：1从大麦衍生的，所述 本发明涉及用于诊断大麦中病毒感染的试剂盒，其包含用于诊断大麦病毒感染的组合物以及使用该组合物或试剂盒诊断大麦病毒感染的方法。 本发明提供的用于诊断大麦病毒感染的组合物或试剂盒可用于诊断迄今为止还不知道的由新病毒引起的大麦感染 这将是。
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公开(公告)号：KR1020140046570A
公开(公告)日：2014-04-21
申请号：KR1020120110742
申请日：2012-10-05
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/686 , C12Q2600/13
Abstract: The present invention provides: a primer set for differentiating the seed coat color of black rice containing anthocyanin and other brown rice seed coat colors; a kit for differentiating the seed coat color of black rice containing anthocyanin and other brown rice seed coat colors; and a method of differentiating the seed coat color of black rice containing anthocyanin and other brown rice seed coat colors by performing an amplification reaction using the primer set. By using the primer set of the present invention, a user can differentiate black rice in a seedling condition compared to an existing sorting method of confirming the color of seed coats after the vegetative growth, discharging water, and grain filling; and the homomorphy and heteromorphy of characteristics can be possible, thereby improving the breeding efficiency utilizing the juvenile selection of black rice and minimum resources. [Reference numerals] (AA) Boseokheugchal(black rice); (BB) No.244 of Miryang(white rice); (CC) Approximately 450 bp; (DD) Approximately 215 bp
Abstract translation: 本发明提供了一种用于分化含有花青素和其他糙米种衣色的黑米的种皮颜色的引物组; 一种用于区分含有花青素和其他糙米种皮颜色的黑米的种皮颜色的试剂盒; 以及通过使用引物组进行扩增反应来区分含有花青素和其他糙米种皮颜色的黑米的种皮颜色的方法。 通过使用本发明的引物组，与用于确认营养生长后的种皮颜色的现有分类方法相比，使用者能够在苗期条件下区分黑米，排出水和灌浆; 特色的同性异体和异质性是可能的，从而通过青少年选择黑米和最低限度的资源提高育种效率。 （AA）Boseokheugchal（黑米）; （BB）米阳244号（白米）; （CC）约450bp; （DD）约215bp
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3.发明授权ＤＮＡ 마커를 포함하는 끝동매미충 저항성 유전자 Ｇｒｈ１을 가진 벼 품종 선별용 조성물 및 ＤＮＡ 마커를 이용한 끝동매미충 저항성 벼 품종 선별 방법 有权用于选择含有DNA标记的含有Grh1基因的绿色稻叶蝉抗性品种的组合物和使用DNA标记选择绿色水稻叶hop抗性品种的方法
公开(公告)号：KR101322345B1
公开(公告)日：2013-10-28
申请号：KR1020110030389
申请日：2011-04-01
Applicant: 대한민국(농촌진흥청장)
Abstract: 본 발명은 RM516, RM6082, RM18182, RM18184, RM18185, RM5844 및 RM7409로 구성되는 군으로부터 선택된 하나 이상의 올리고뉴클레오티드 또는 이의 단편의 존재여부를 검출하는 프라이머를 포함하는, 끝동매미충 저항성 벼 품종 선별용 조성물, 구체적으로는, 서열번호 1 내지 서열번호 14로 구성되는 군으로부터 선택되는 하나 이상의 프라이머 세트를 포함하는, 끝동매미충 저항성 벼 품종 선별용 조성물에 관한 것이다. 또한, 본 발명은 벼 시료에서 게놈 DNA를 분리하는 단계; 상기 분리된 게놈 DNA를 주형으로 하고, 서열번호 1 내지 서열번호 14로 구성되는 군으로부터 선택되는 하나 이상의 프라이머 세트를 이용하여 증폭 반응을 수행하여 표적 서열을 증폭하는 단계; 및 상기 증폭 산물을 검출하는 단계를 포함하는, 끝동매미충 저항성 벼 품종 선별 방법 및 벼 선별 키트에 관한 것이다. 본 발명에 따른 끝동매미충 저항성 개체를 선발하는 방법을 이용하면, 끝동매미충 저항성 유전자의 위치와 아주 밀접하게 연관된 마커를 이용하여 효율적으로 끝동매미충 저항성 및 감수성 개체를 구분할 수 있다.
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4.发明公开
公开(公告)号：KR1020130091434A
公开(公告)日：2013-08-19
申请号：KR1020120012710
申请日：2012-02-08
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6895 , C12Q1/6844 , C12Q2600/13 , C12Q2600/158
Abstract: PURPOSE: A method of selecting a breed that is resistive to a rice stripe disease is provided to efficiently separate a breed that is resistive and sensitive with respect to the rice stripe disease by using a marker, which is closely related with a location of a rice stripe disease-resistant gene. CONSTITUTION: A pair of primers for selecting a breed of rice resistive against a rice stripe disease is selected from the group consisting of a primer pair represented by SEQ ID NO: 1 and 2; a primer pair represented by SEQ IE NO: 3 and 4; a primer pair represented by SEQ IE NO: 5 and 6; a primer pair represented by SEQ IE NO: 7 and 8; a primer pair represented by SEQ IE NO: 9 and 10; a primer pair represented by SEQ IE NO: 11 and 12; a primer pair represented by SEQ IE NO: 13 and 14; a primer pair represented by SEQ IE NO: 15 and 16; a primer pair represented by SEQ IE NO: 17 and 18; a primer pair represented by SEQ IE NO: 19 and 20; and a primer pair represented by SEQ IE NO: 21 and 22. A method of selecting a rice stripe disease-resistant breed comprises the steps of: (i) isolating DNA from a rice sample; (ii) amplifying a target sequence by performing an amplification reaction by having the isolated DNA as a template and using the at least one primer pair selected from above; and (iii) detecting the amplification product.
Abstract translation: 目的：提供一种选择抗稻瘟病抗性品种的方法，通过使用与稻米位置密切相关的标记，有效地分离出与水稻条纹病有关的抗性和敏感性品种 条纹抗病基因。 构成：从由SEQ ID NO：1和2表示的引物对组成的组中选择一对用于选择抗稻瘟病抗性的水稻品种的引物; 由SEQ ID NO：3和4表示的引物对; 由SEQ ID NO：5和6表示的引物对; 由SEQ ID NO：7和8表示的引物对; 由SEQ ID NO：9和10表示的引物对; 由SEQ ID NO：11和12表示的引物对; 由SEQ ID NO：13和14表示的引物对; 由SEQ ID NO：15和16表示的引物对; 由SEQ ID NO：17和18表示的引物对; 由SEQ ID NO：19和20表示的引物对; 和由SEQ ID NO：21和22表示的引物对。选择水稻条纹抗病品种的方法包括以下步骤：（i）从稻样品中分离DNA; （ii）通过使分离的DNA作为模板并使用选自上述的至少一种引物对进行扩增反应来扩增靶序列; 和（iii）检测扩增产物。
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公开(公告)号：KR1020120037585A
公开(公告)日：2012-04-20
申请号：KR1020100099131
申请日：2010-10-12
Applicant: 대한민국(농촌진흥청장)
Abstract: PURPOSE: A cosmetic composition containing black waxy rice extract is provided to ensure excellent anti-wrinkling and skin whitening. CONSTITUTION: A cosmetic composition for anti-wrinkling and skin whitening contains 0.01-10 wt% of black waxy rice extract as an active ingredient. The extract is prepared using water, ethanol, methanol, or ethyl acetate. The extract contains anthocyanine, amylo pectin, GABA, gamma-oryzanol, proteins, fat and fatty acid, inorganic ingredients, amino acids, saccharides, and vitamins.
Abstract translation: 目的：提供含有黑糯米提取物的化妆品组合物，以确保优异的抗皱和皮肤美白。 构成：抗皱和皮肤美白化妆品组合物含有0.01-10重量％的黑糯米提取物作为活性成分。 萃取液用水，乙醇，甲醇或乙酸乙酯制备。 提取物含有花青，淀粉果胶，GABA，γ-谷维素，蛋白质，脂肪和脂肪酸，无机成分，氨基酸，糖类和维生素。
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Patent Agency Ranking
公开(公告)号：KR101869795B1
公开(公告)日：2018-06-21
申请号：KR1020160082336
申请日：2016-06-30
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/70 , G01N33/569
Abstract: 본발명은보리로부터유래된서열번호 1의염기서열을포함하는신규한바이러스유전자, 상기신규한바이러스유전자또는상기유전자로부터발현된단백질을검출할수 있는제제를포함하는보리의바이러스감염여부진단용조성물, 상기보리의바이러스감염여부진단용조성물을포함하는보리의바이러스감염여부진단용키트및 상기조성물또는키트를이용하여보리의바이러스감염여부를진단하는방법에관한것이다. 본발명에서제공하는보리의바이러스감염여부진단용조성물또는키트를사용하면, 지금까지알려지지않았던신규한바이러스에의한보리의감염여부를진단할수 있으므로, 바이러스감염에의한보리의질병발생여부의판단에널리활용될수 있을것이다.
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公开(公告)号：KR101794697B1
公开(公告)日：2017-11-07
申请号：KR1020150157555
申请日：2015-11-10
Applicant: 대한민국(농촌진흥청장)
Abstract: 본발명은보리누른모자이크병바이러스, 보리마일드모자이크병바이러스또는보리위축병바이러스에특이적인프라이머세트들을포함하는보리바이러스병의진단용조성물, 상기조성물을포함하는진단용키트, 상기조성물을이용하여 RT-PCR을수행하는단계를포함하는, 보리바이러스병의진단방법에관한것이다. 본발명의진단용조성물은국내에주로발생하는보리바이러스병 2종이상을동시에진단할수 있으므로, 진단에필요한시간및 노력을절감할수 있으며, 보리바이러스병의발생또는확산을예방할수 있을뿐만아니라, 나아가맥류바이러스병의종합관리체계를확립하는데기여할수 있을것이다.
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公开(公告)号：KR1020170054872A
公开(公告)日：2017-05-18
申请号：KR1020150157555
申请日：2015-11-10
Applicant: 대한민국(농촌진흥청장)
Abstract: 본발명은보리누른모자이크병바이러스, 보리마일드모자이크병바이러스또는보리위축병바이러스에특이적인프라이머세트들을포함하는보리바이러스병의진단용조성물, 상기조성물을포함하는진단용키트, 상기조성물을이용하여 RT-PCR을수행하는단계를포함하는, 보리바이러스병의진단방법에관한것이다. 본발명의진단용조성물은국내에주로발생하는보리바이러스병 2종이상을동시에진단할수 있으므로, 진단에필요한시간및 노력을절감할수 있으며, 보리바이러스병의발생또는확산을예방할수 있을뿐만아니라, 나아가맥류바이러스병의종합관리체계를확립하는데기여할수 있을것이다.
Abstract translation: 本发明大麦向下花叶病病毒，大麦温和花叶病病毒或大麦大麦卷曲病毒疾病的诊断组合物包括特定的引物组上的病毒，诊断试剂盒包含所述组合物，使用该组合物的RT-PCR 一种诊断大麦病毒病的方法。 本发明的诊断组合物，因为它可以诊断小麦病毒性疾病两种或更多种的发生主要在国内同时，并可以减少诊断所需的时间和精力，以及可以防止的发生或小麦病毒性疾病的蔓延，此外静脉曲张 它将有助于建立一个全面的病毒性疾病管理系统。
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公开(公告)号：KR1020120111806A
公开(公告)日：2012-10-11
申请号：KR1020110030391
申请日：2011-04-01
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6895 , C12Q1/6844 , C12Q2600/13
Abstract: PURPOSE: A primer set for determining white rice and colored rice is provided to determine homo/hetero type of characteristics and to enhance breeding efficiency. CONSTITUTION: A primer set for determining white rice and colored rice containing proanthocyanidin-based pigment comprises: an oligonucleotide primer set of sequence numbers 1 and 2; an oligonucleotide primer set of sequence numbers 3 and 4; an oligonucleotide primer set of sequence numbers 5 and 6; an oligonucleotide primer set of sequence numbers 7 and 4; and an oligonucleotide primer set of sequence numbers 8 and 6. A kit for determining the seed coat color of white rice and colored rice contains the primer set and reagent.
Abstract translation: 目的：提供确定白米和彩色米的引物组，以确定特异性/杂种类型，提高繁殖效率。 构成：用于测定含有原花青素色素的白米和色米的引物组包括：序列号1和2的寡核苷酸引物组; 序列号3和4的寡核苷酸引物组; 序列号5和6的寡核苷酸引物组; 序列号7和4的寡核苷酸引物组; 和序列号8和6的寡核苷酸引物组。用于测定白米和有色米的种皮颜色的试剂盒含有引物组和试剂。
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10.发明公开ＤＮＡ 마커를 포함하는 끝동매미충 저항성 유전자 Ｇｒｈ１을 가진 벼 품종 선별용 조성물 및 ＤＮＡ 마커를 이용한 끝동매미충 저항성 벼 품종 선별 방법 有权用于选择含有包含DNA标记的GRH1基因的绿色耐旱变种的组合物和使用DNA标记选择绿色耐旱叶片的变种的方法
公开(公告)号：KR1020120111804A
公开(公告)日：2012-10-11
申请号：KR1020110030389
申请日：2011-04-01
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12Q1/6895 , C12Q1/683 , C12Q2600/13
Abstract: PURPOSE: A composition containing a DNA marker for selecting Oryza sativa with green rice leaf hopper-resistant gene Grh1 is provided to efficiently distinguish green rice leaf hopper resistant and sensitive individual. CONSTITUTION: A composition for selecting Oryza sativa species with green rice leaf hopper-resistance contains a primer for detecting the presence of an oligonucleotide or a fragment thereof. The primer is one or more primer sets selected from sequence numbers 1-14. The primer set is one or more primer sets selected from sequence numbers 3-14. A method for selecting the Oryza sativa species comprises: a step of isolating genome DNA from Oryza sativa samples; a step of amplifying a target sequence using the genome DNA as a template and the primer set; and a step of detecting the amplifies product. The sample is derived from roots, leaves, stems, flowers, or buds of Oryza sativa. A kit for selecting Oryza sativa species with green rice leaf hopper-resistance contains the primer set selected from sequence numbers 1-14, restriction enzyme, and reagent.
Abstract translation: 目的：提供含有绿色稻叶漏斗抗性基因Grh1的选择水稻的DNA标记物的组合物，以有效区分绿色稻叶斗漏斗和敏感个体。 构成：用绿色稻叶料斗抗性选择水稻种的组合物含有用于检测寡核苷酸或其片段的存在的引物。 引物是选自序列号1-14中的一个或多个引物组。 引物组是选自序列号3-14的一个或多个引物组。 选择水稻种的方法包括：从水稻样品中分离基因组DNA的步骤; 使用基因组DNA作为模板和引物组扩增靶序列的步骤; 以及检测放大产物的步骤。 样品来源于水稻的根，叶，茎，花或芽。 用于选择具有绿色稻叶料斗抗性的水稻品种的试剂盒含有选自序列号1-14，限制酶和试剂的引物组。
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