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公开(公告)号：KR101892085B1
公开(公告)日：2018-08-27
申请号：KR1020170097855
申请日：2017-08-01
Applicant: 대한민국(농촌진흥청장) , 한림대학교 산학협력단
IPC: C12Q1/68
CPC classification number: C12Q1/6888 , C12Q2527/101 , C12Q2563/107
Abstract: 본발명은벼멸구검출용고리매개등온증폭용프라이머세트및 이를이용한벼멸구검출방법관한것으로, 본발명의프라이머세트는벼멸구()을 PCR 방법보다빠르게특이적으로검출할수 있고전기영동없이형광반응으로신속하게파악하여방제시기결정에활용할수 있다.
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公开(公告)号：KR102159023B1
公开(公告)日：2020-09-23
申请号：KR1020190131684
申请日：2019-10-22
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/6876 , C12Q1/6888
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公开(公告)号：KR100404767B1
公开(公告)日：2003-11-07
申请号：KR1020010016649
申请日：2001-03-29
Applicant: 대한민국(농촌진흥청장) , 윤철식
IPC: C12N1/20
Abstract: PURPOSE: A microorganism Verticillium lecanii CS626 and a method for the control of harmful insects using the same microorganism are provided, thereby effectively preventing the growth of the harmful insects living in agricultural equipments. CONSTITUTION: The microorganism Verticillium lecanii CS626(KFCC-11277) has an improved insecticidal effect on the harmful insects living in agricultural equipments, wherein it is isolated by collecting a mycelium from the surface of an insect infected by Verticillium lecanni; inoculating the mycelium on a potato agar medium containing tetracycline; culturing it at 22 to 28 deg. C; isolating spores and mycelia from the cultured medium; suspending the isolated spores in 0.01% tween 80 solution; spraying the suspension on a leaf containing insects; and selecting a microorganism showing excellent insecticidal activity.
Abstract translation: 目的：提供一种微生物蜡蚧轮枝菌（Verticillium lecanii）CS626和使用相同微生物控制有害昆虫的方法，从而有效地防止住在农业设备中的有害昆虫的生长。 组成：微生物蜡蚧轮枝菌Verticillium lecanii CS626（KFCC-11277）对农业设备中的有害昆虫具有改善的杀虫效果，其中通过从被轮枝孢菌感染的昆虫表面收集菌丝体来分离它; 将菌丝体接种在含有四环素的马铃薯琼脂培养基上; 在22至28度培养它。 C; 从培养基中分离孢子和菌丝体; 将分离的孢子悬浮在0.01％吐温80溶液中; 将悬浮液喷洒在含有昆虫的叶上; 并选择显示优异的杀虫活性的微生物。
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Patent Agency Ranking6.发明授权애멸구 유래 재조합 그로이엘단백질 유전자 클론 및 이를이용한 벼줄무늬 잎마름병 바이러스의 면역포획역전사효소연쇄중합반응 검정방법 有权来自小褐斑病病人的重组体基因克隆和使用蛋白质蛋白质的米糠病毒免疫测定RT-PCR的检测方法
公开(公告)号：KR100822742B1
公开(公告)日：2008-04-17
申请号：KR1020070026577
申请日：2007-03-19
Applicant: 대한민국(농촌진흥청장)
CPC classification number: C12N15/11 , C12Q1/6888 , C12Q1/701 , C12Q2531/113
Abstract: A recombinant protein derived from small brown planthopper is provided to be specifically coupled to thread-shaped rice stripe virus, thereby forecasting precise RSV outbreak, developing a transgenic plant having RSV resistance and being applied as various tools for detecting the RSV. A recombinant protein described in SEQ ID : NO. 2 is obtained by expressing a recombinant protein in a gene clone(deposition no. KACC-95060P) described in SEQ ID : NO. 1, which is constructed by inserting a GroEL gene isolated from small brown planthopper into an expression vector, and then purifying the protein using His-taq Ni-NTA agarose column and is characterized in that it is specifically coupled to thread-shaped RSV. A method for detecting an RSV gene comprises the steps of: (a) coating a reaction container which is PolySorp treated with the GroEL recombinant protein derived from the small brown planthopper or an antibody thereof with a coating solution; (b) treating the reaction solution with a detecting sample through coupling reaction with the with the RSV to immuno-capturing RSV particles; and (c) after isolating genes from the immuno-captured RSV particles, subjecting the isolated genes to RT-PCR to detect genes of the RSV.
Abstract translation: 提供了一种来源于小型褐飞虱的重组蛋白，特异性地与丝状水稻条纹病毒偶联，从而预测了精确的RSV疫情，开发了具有RSV抗性的转基因植物，并被用作检测RSV的各种工具。 SEQ ID NO： 2是通过在SEQ ID NO：1中描述的基因克隆（沉积编号KACC-95060P）中表达重组蛋白获得的。 1，其通过将从小褐飞虱分离的GroEL基因插入表达载体中构建，然后使用His-taq Ni-NTA琼脂糖柱纯化蛋白质，其特征在于其特异性结合于螺纹状RSV。 检测RSV基因的方法包括以下步骤：（a）用来自小褐飞虱或其抗体的GroEL重组蛋白用涂布溶液涂覆PolySorp处理的反应容器; （b）用检测样品通过与RSV的偶联反应处理反应溶液以免疫捕获RSV颗粒; 和（c）从免疫捕获的RSV颗粒中分离出基因后，将分离的基因进行RT-PCR检测RSV的基因。
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7.发明公开버티실리움 레카니 씨에스626 국내 토착균주 및 이를이용한 시설해충의 방제방법 有权MICROORGANISM VERTICILLIUM LECANII CS626及使用该方法控制有害物质的方法
公开(公告)号：KR1020020076646A
公开(公告)日：2002-10-11
申请号：KR1020010016649
申请日：2001-03-29
Applicant: 대한민국(농촌진흥청장) , 윤철식
IPC: C12N1/20
Abstract: PURPOSE: A microorganism Verticillium lecanii CS626 and a method for the control of harmful insects using the same microorganism are provided, thereby effectively preventing the growth of the harmful insects living in agricultural equipments. CONSTITUTION: The microorganism Verticillium lecanii CS626(KFCC-11277) has an improved insecticidal effect on the harmful insects living in agricultural equipments, wherein it is isolated by collecting a mycelium from the surface of an insect infected by Verticillium lecanni; inoculating the mycelium on a potato agar medium containing tetracycline; culturing it at 22 to 28 deg. C; isolating spores and mycelia from the cultured medium; suspending the isolated spores in 0.01% tween 80 solution; spraying the suspension on a leaf containing insects; and selecting a microorganism showing excellent insecticidal activity.
Abstract translation: 目的：提供微生物轮枝孢菌CS626和使用相同微生物控制害虫的方法，从而有效防止生活在农业设备中的有害昆虫的生长。 构成：微生物轮枝孢属植物CS626（KFCC-11277）对农业设备中的有害昆虫具有改善的杀虫作用，其通过从轮枝堇病毒感染的昆虫表面收集菌丝来分离， 将菌丝体接种在含有四环素的马铃薯琼脂培养基上; 在22〜28度培养 C; 从培养基中分离孢子和菌丝体; 将分离的孢子悬浮在0.01％吐温80溶液中; 将悬浮液喷洒在含有昆虫的叶上; 并选择显示出优异的杀虫活性的微生物。
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公开(公告)号：KR102000459B1
公开(公告)日：2019-07-16
申请号：KR1020180016254
申请日：2018-02-09
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/6888
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公开(公告)号：KR102207783B1
公开(公告)日：2021-01-26
申请号：KR1020190166776
申请日：2019-12-13
Applicant: 대한민국(농촌진흥청장)
IPC: C12Q1/6888
Abstract: 본발명은선녀벌레집게벌의종 판별을위한 PCR용프라이머세트및 이를이용하여선녀벌레집게벌종을판별하는방법에관한것이다. 본발명에따른선녀벌레집게벌특이프라이머세트를이용하면선녀벌레집게벌의종 판별이가능하여산란초기에서도약충단계의미국선녀벌레에서선녀벌레집게벌의산란및 기생여부를확인하는것이가능한바, 초기에미국선녀벌레의천적기생율조사가가능하고생물적방제전략수립에기생율정보를제공할수 있으며, 선녀벌레집게벌을활용한미국선녀벌레의생물적방제에서정확한해충방제효과를산출할수 있을것으로기대된다. 또한미국선녀벌레와유사종인선녀벌레에서도선녀벌레집게벌의산란및 기생가능성을조기에판별해선녀벌레집게벌의선녀벌레방제에활용여부를판단할수 있을것으로기대된다.
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公开(公告)号：KR100475346B1
公开(公告)日：2005-03-10
申请号：KR1020020040749
申请日：2002-07-12
Applicant: 대한민국(농촌진흥청장) , 학교법인고려중앙학원
IPC: C12N1/14
Abstract: 본 발명은 국내 토착 균주
버티실리움 레카니 CS625(
Verticillium lecanii CS625) 및 이를 이용한 진딧물 방제 방법에 관한 것이다. 보다 구체적으로는, 진딧물에 대하여 살충력이 우수하고 시설 재배지 환경조건에서도 잘 적응된 국내 토착 균주
버티실리움 레카니 CS625 균주, 상기 균주 및 균주의 포자 현탁액을 함유하는 진딧물 방제용 조성물 및 상기 조성물을 농작물에 살포하여 진딧물, 특히 목화진딧물 및 복숭아혹진딧물을 생물학적으로 방제하는 방법에 관한 것이다. 본 발명 균주는 복숭아혹진딧물로부터 분리한 국내 토착 균주로, 시설 재배지의 환경조건에서도 생장률이 우수할 뿐만 아니라, 상기 균주 및 균주로부터 생성된 포자현탁액을 시설 재배재의 농작물에 처리 시 목화진딧물을 효과적으로 방제할 수 있는 뛰어난 효과가 있다.
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