公开(公告)号：KR1020020094646A

公开(公告)日：2002-12-18

申请号：KR1020010032990

申请日：2001-06-12

Applicant: 대한민국(서울대학총장)

Inventor： 김기호 ,  이재섭 ,  김기수 ,  조종수 ,  조광용 ,  이광길 ,  여주홍 ,  이용우

IPC: C07D207/46 ,  B82Y40/00

Abstract: PURPOSE: Provided is a method for producing self-assembled nanoparticle of sericine which can be effectively used in functional cosmetics and a drug delivery system. CONSTITUTION: The method for producing self-assembled nanoparticle of sericine comprises the steps of: reaction of sericine extracted from cocoons with 2-(O-methoxypolyethylene glycol)- 4,6-dichloro- S-triazine (actPEG1) or 2,4-bis (O-methoxypolyethylene glycol)-6 -chloro-S-triazine (actPEG2) in a base buffer solution; and dialysis of a resulting product, wherein the sericine is extracted from cocoons in hot water of 40 to 120 deg. C or treated by enzyme to have molecular weight of 1,000 to 20,000 dalton; the reaction is carried out at 4 to 40 deg. C for 3 to 36 hours in the presence of base buffer solution; and the base buffer solution is selected from the group consisting of a boron salt buffer solution, tris buffer solution, AMPSO buffer solution, CHES buffer solution, CAPSO buffer solution, AMP buffer solution, and CAPS buffer solution.

Abstract translation: 目的：提供一种可以有效用于功能性化妆品和药物输送系统的丝氨酸自组装纳米粒子的制备方法。 构成：用于生产丝氨酸自组装纳米粒子的方法包括以下步骤：从茧提取的丝胶与2-（O-甲氧基聚乙二醇）-4,6-二氯-S-三嗪（actPEG1）或2,4- 双（O-甲氧基聚乙二醇）-6-氯-3-三嗪（actPEG2）; 并对所得产物进行透析，其中将丝氨酸在40至120度的热水中从茧中提取。 或用酶处理分子量为1,000至20,000道尔顿; 反应在4至40℃进行。 在碱缓冲溶液存在下3至36小时; 碱缓冲溶液选自硼盐缓冲溶液，tris缓冲溶液，AMPSO缓冲溶液，CHES缓冲溶液，CAPSO缓冲溶液，AMP缓冲溶液和CAPS缓冲溶液。

公开(公告)号：KR100449904B1

公开(公告)日：2004-09-22

申请号：KR1020010032990

申请日：2001-06-12

Applicant: 대한민국(서울대학총장)

Inventor： 김기호 ,  이재섭 ,  김기수 ,  조종수 ,  조광용 ,  이광길 ,  여주홍 ,  이용우

IPC: C07D207/46 ,  B82Y40/00

Abstract: PURPOSE: Provided is a method for producing self-assembled nanoparticle of sericine which can be effectively used in functional cosmetics and a drug delivery system. CONSTITUTION: The method for producing self-assembled nanoparticle of sericine comprises the steps of: reaction of sericine extracted from cocoons with 2-(O-methoxypolyethylene glycol)- 4,6-dichloro- S-triazine (actPEG1) or 2,4-bis (O-methoxypolyethylene glycol)-6 -chloro-S-triazine (actPEG2) in a base buffer solution; and dialysis of a resulting product, wherein the sericine is extracted from cocoons in hot water of 40 to 120 deg. C or treated by enzyme to have molecular weight of 1,000 to 20,000 dalton; the reaction is carried out at 4 to 40 deg. C for 3 to 36 hours in the presence of base buffer solution; and the base buffer solution is selected from the group consisting of a boron salt buffer solution, tris buffer solution, AMPSO buffer solution, CHES buffer solution, CAPSO buffer solution, AMP buffer solution, and CAPS buffer solution.

Abstract translation: 目的：提供一种生产可以有效用于功能性化妆品和药物递送系统的丝氨酸自组装纳米粒子的方法。 构成：生产自组装纳米丝胶的方法包括以下步骤：将从茧中提取的丝胶与2-（O-甲氧基聚乙二醇）-4,6-二氯-S-三嗪（actPEG1）或2,4- （O-甲氧基聚乙二醇）-6-氯-S-三嗪（actPEG2）在碱缓冲溶液中的溶液; 并对所得产物进行透析，其中丝胶在40至120℃的热水中从茧中提取。 或通过酶处理以具有1,000至20,000道尔顿的分子量; 反应在4至40℃下进行。 在碱缓冲溶液存在下进行3至36小时; 并且碱缓冲溶液选自由硼盐缓冲溶液，tris缓冲溶液，AMPSO缓冲溶液，CHES缓冲溶液，CAPSO缓冲溶液，AMP缓冲溶液和CAPS缓冲溶液组成的组。