公开(公告)号：KR1020000058378A

公开(公告)日：2000-10-05

申请号：KR1020000024818

申请日：2000-05-10

Applicant: 대한민국(충남대학교)

Inventor： 송경빈 ,  원미선 ,  김소연 ,  이승환 ,  김철중 ,  전무형 ,  김현수

IPC: C12N15/70

CPC classification number: C12N15/70 ,  A61K38/16

Abstract: PURPOSE: Provided is MBP-agfA fused protein to mass-expressed agfA, which is Salmonella enteritidis fimbriae protein, in E.coli. CONSTITUTION: Samonella enteritidis is composed SEF 21, 17 and 14 fimbriae proteins, in which SEF17 consisting of agfA has an excellent antigen property and a potentiality to be used as vaccine against infection of Salmonella. The agfA subunit of Salmonella enteritidis fimbriae itself is not water-soluble, thereby it is not easy to apply to vaccine. The agfA subunit is fused with maltose binding protein in order to be soluble in water. The agfA subunit fused with maltose binding protein is mass-expressed in E. coli.

Abstract translation: 目的：在大肠杆菌中提供MBP-agfA融合蛋白与质粒表达的agfA，其是肠炎沙门氏菌菌毛蛋白。 构成：肠炎沙门氏菌组成SEF 21,17和14菌毛蛋白，其中由agfA组成的SEF17具有优良的抗原性能和潜在的抗沙门氏菌感染疫苗。 沙门氏肠杆菌菌毛本身的agfA亚基不是水溶性的，因此不易于应用于疫苗。 agfA亚基与麦芽糖结合蛋白融合，以溶于水。 与麦芽糖结合蛋白融合的agfA亚基在大肠杆菌中大量表达。