公开(公告)号：KR1020140071059A

公开(公告)日：2014-06-11

申请号：KR1020120138966

申请日：2012-12-03

Applicant: 대한민국(해양수산부 국립수산물품질관리원장)

Inventor： 황창남 ,  황은미 ,  오윤철 ,  윤상린 ,  서장우

IPC: C12N15/11 ,  C12Q1/68

CPC classification number: C12Q1/686 ,  C12N15/11 ,  C12Q1/683

Abstract: The present invention relates to a primer, capable of identifying species which pertain to the Branchiostegus family, a method for identifying species of the Branchiostegus family using the same, and a polynucleotide useful in identifying species of the Branchiostegus family. Branchiostegus japonicas and Branchiostegus albus can be identified when a polynucleotide having a pair of nucleotides of SEQ ID NO: 3, 5, or 7 and SEQ ID NO: 4, 6, or 8 is used. Branchiostegus argentatus and Branchiostegus auratus can be identified when a single nucleotide polymorphism (SNP) marker of SEQ ID NO: 12 or 13 is used.

Abstract translation: 本发明涉及一种能够鉴定与Branch科家族有关的物种的引物，用于鉴定使用其的Branch科家族物种的方法，以及可用于鉴定Branch科家族物种的多核苷酸。 当使用具有SEQ ID NO：3,5或7对核苷酸和SEQ ID NO：4,6或8的核苷酸的多核苷酸时，可以鉴定出Branch us属（Logiostegus japonicas）和Branch属（Branchiostegus albus）。 当使用SEQ ID NO：12或13的单核苷酸多态性（SNP）标记时，可以鉴定分枝杆菌属（Archioctgus argentatus）和斑节杆菌（Branchiostegus auratus）。

公开(公告)号：KR101395344B1

公开(公告)日：2014-05-16

申请号：KR1020130125069

申请日：2013-10-21

Applicant: 대한민국(해양수산부 국립수산물품질관리원장)

Inventor： 황창남 ,  황은미 ,  이정선

IPC: C12N15/11 ,  C12Q1/68

CPC classification number: C12N15/11 ,  C12Q1/6813 ,  C12Q1/6818 ,  C12Q2525/107 ,  C12Q2527/107

Abstract: The present invention relates to a peptide nucleic acid set for identifying skate ray species and a skate ray species identification method using the same, and more specifically, to a method of identifying skate ray species by hybridizing more than one peptide nucleic acid (PNA) composed of more than one base sequence selected from a base sequence group composed of SEQ ID NO: 1-6 with a skate ray DNA sample and obtaining a melting curve per temperature by increasing the temperature of the hybridized product. From the obtained melting temperature of the melting curve, it is possible to identify the skate ray species. The present invention as described above uses PNA having an excellent binding force with DNA so as to indicate different melting temperatures for each skate ray species, thereby having the effects of simply, promptly, and accurately identifying the skate ray species.

Abstract translation: 本发明涉及用于识别溜冰鞋类物种的肽核酸组和使用其的溜冰溜溜球物种识别方法，更具体地说，涉及一种通过杂交多于一种的肽核酸（PNA）来鉴定溜冰鞋类物种的方法 的多个碱基序列，其选自由SEQ ID NO：1-6组成的碱基序列组和溜冰鞋DNA样品，并通过增加杂交产物的温度获得每温度的熔解曲线。 根据获得的熔解曲线的熔融温度，可以识别滑冰射线种类。 如上所述的本发明使用与DNA具有优异结合力的PNA，以表示对于每个溜冰射线物种的不同熔融温度，从而具有简单，迅速和准确地识别溜冰鞋类物种的效果。

公开(公告)号：KR101534271B1

公开(公告)日：2015-07-09

申请号：KR1020120138966

申请日：2012-12-03

Applicant: 대한민국(해양수산부 국립수산물품질관리원장)

Inventor： 황창남 ,  황은미 ,  오윤철 ,  윤상린 ,  서장우

IPC: C12N15/11 ,  C12Q1/68

Abstract: 본발명은옥돔()과에속하는종의품종을판별할수 있는프라이머, 이를이용한옥돔과품종판별방법및 옥돔과품종판별에유용한폴리뉴클레오티드에대한것으로, 서열번호 3, 5 또는 7 및서열번호 4, 6 또는 8의염기서열을쌍으로가지는폴리뉴클레오티드를프라이머로사용하는경우옥돔()과옥두어()를판별할수 있고, 서열번호 12 또는 13의단일뉴클레오타이드다형성(SNP, Single Nucleotide Polymorphism) 마커를이용하면등흑점옥두어()과황옥돔()을정확하고간단하게판별할수 있는효과가있다.