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公开(公告)号：KR1020160014960A
公开(公告)日：2016-02-12
申请号：KR1020140097192
申请日：2014-07-30
Applicant: 대한민국 (식품의약품안전처장)
IPC: C12Q1/68
CPC classification number: C12Q1/6844
Abstract: 본발명은식중독미생물의병원성유전자동시검출용프라이머세트및 이의용도에관한것으로, 보다상세하게는식중독미생물의병원성유전자인 mapA, ceuE, cpe, ctx, glnA, tdh, bceT, ail, coa, his, iap, VT1, VT2, STh 및 STp의동시검출이가능한프라이머세트및 이의용도에관한것이다. 본발명의식중독미생물의병원성유전자동시검출용프라이머세트는쉽고빠르며민감도와특이도가높으므로식중독미생물의병원성유전자검출에있어서효과적이다.
Abstract translation: 本发明涉及同时检测导致食物中毒的微生物的毒力基因的引物组及其用途。 更具体地，本发明涉及确保同时检测作为毒力的mapA，ceuE，cpe，ctx，glnA，tdh，bceT，ail，coa，his，iap，VT1，VT2，STh和STp的引物组 导致食物中毒的微生物基因。 本发明还涉及其用途。 用于同时检测导致食物中毒的微生物毒力基因的引物组快速且易于使用，并且具有高灵敏度和特异性。 因此，引物组在检测导致食物中毒的微生物的毒力基因方面是有效的。 为此，本发明的引物组包括由各种序列号1至45表示的特异性引物组。
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公开(公告)号：KR101653161B1
公开(公告)日：2016-09-02
申请号：KR1020120129698
申请日：2012-11-15
Applicant: 중앙대학교 산학협력단 , 대한민국 (식품의약품안전처장)
Abstract: 본발명은육류에서 E형간염바이러스(Hepatitis E virus, HEV)의검출방법에관한것이다. 보다상세하게는, 본발명은 HEV의오픈리딩프레임 (Open reading frame) 서열에서가장감도와특이성이높은서열을기초로하여설계한유전자특이적인 HEV 검출용프라이머세트, 상기프라이머및 프로브세트를이용하여실시간 RT-PCR 을수행하며돼지간장에서 E형간염바이러스의유전자를검출할시 검출률이낮은것을고려하여시료에대해바이러스탈리및 농축과정을수행하여 HEV를검출하는방법에관한것이다. 본발명의 HEV 탈리및 농축방법과검출용조성물을이용하는경우, HEV에대한농축률을높여민감도와특이도를높일수 있으며시간과노력을줄이며한 번에많은양의시료를검사할수 있어종래의 HEV 검출방법을대체할수 있는장점이있다.
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公开(公告)号：KR1020140065540A
公开(公告)日：2014-05-30
申请号：KR1020120129698
申请日：2012-11-15
Applicant: 중앙대학교 산학협력단 , 대한민국 (식품의약품안전처장)
CPC classification number: C12Q1/706 , C12Q1/6844 , C12Q1/6888 , C12Q2561/101 , C12Q2561/113
Abstract: The present invention relates to a method for detecting a hepatitis E virus (HEV) in meat. More specifically, the present invention relates to a method for detecting an HEV by performing an RT-PCR using a gene-specific HEV-detecting primer set that is designed based on the most sensitive and specific sequence from among open reading frame sequences of the HEV, the primer, and a probe set and by performing a virus desorbing and concentrating process for a sample by considering that a detection rate is low when a gene of the HEV is detected in swine intestines. If a method for desorbing and concentrating an HEV and a detecting composition are used, a concentration rate for HEV is increased so that sensitivity and specificity can be enhanced. Time and effort can be reduced and a large amount of samples can be inspected at once so that the method can replace existing HEV detecting methods.
Abstract translation: 本发明涉及一种检测肉中戊型肝炎病毒（HEV）的方法。 更具体地，本发明涉及通过使用基于特异性HEV检测引物组进行RT-PCR检测HEV的方法，所述基因特异性HEV检测引物组是基于来自HEV的开放阅读框序列中最敏感和特异性序列设计的 ，引物和探针组，并且通过考虑当在猪肠中检测到HEV的基因时检测率低时，通过对样品进行病毒解吸和浓缩处理。 如果使用HEV和检测组合物的解吸和浓缩方法，HEV的浓度增加，从而可以提高灵敏度和特异性。 可以减少时间和精力，并可以立即检查大量样品，以便该方法可以替代现有的HEV检测方法。
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