-
公开(公告)号：KR1020100041018A
公开(公告)日：2010-04-22
申请号：KR1020080099994
申请日：2008-10-13
Applicant: 대한민국 (식품의약품안전처장)
IPC: C12Q1/68
CPC classification number: G01N33/53 , C12Q1/6837 , C12Q2600/142
Abstract: PURPOSE: A method for determining food additive having hepatotoxicity is provided to effectively determine hepatotoxicity and develop safe food additive. CONSTITUTION: A method for determining food additive having hepatotoxicity comprises: a step of treating food additive to liver cell line and culturing; a step of isolating RNA from liver cell line and synthesizing cDNA; and a step of measuring hepatotoxicity-specific gene expression level and comparing with control liver cell line to determine presence of hepatotoxicity. The liver cell line is HepG2. The measurement is performed through Northern blot.
Abstract translation: 目的：提供一种确定具有肝毒性的食品添加剂的方法，以有效确定肝毒性并开发安全的食品添加剂。 构成：一种确定具有肝毒性的食品添加剂的方法，包括：对肝细胞系的食品添加剂进行处理和培养的步骤; 从肝细胞系中分离RNA并合成cDNA的步骤; 测定肝毒性特异性基因表达水平，与对照肝细胞系比较，测定肝毒性的存在。 肝细胞系为HepG2。 通过Northern印迹进行测量。
-
公开(公告)号：KR1020100040138A
公开(公告)日：2010-04-19
申请号：KR1020080099228
申请日：2008-10-09
Applicant: 대한민국 (식품의약품안전처장)
IPC: C12Q1/68
CPC classification number: C12Q1/6837 , C12Q2600/142
Abstract: PURPOSE: A method for determining medicinal product having mytotoxicity is provided to effectively determine mytotoxicity and develop safe drug. CONSTITUTION: A method for determining medicinal product having mytotoxicity comprises: a step of treating medicinal product to muscular cells and culturing the cells; a step of isolating RNA form the cultured cells and synthesizing cRNA; and a step of measuring expression level of mytotoxicity-specific gene in the synthesized cRNA and comparing cRNA of untreated group. The expression level of the gene is performed through micorarray.
Abstract translation: 目的：提供一种确定具有肌肉毒性的药物的方法，以有效测定肌肉毒性并开发安全药物。 构成：用于测定具有肌肉毒性的药物的方法，其特征在于，包括：对肌肉细胞处理药物并培养细胞的步骤; 分离RNA形成培养细胞并合成cRNA的步骤; 并测定合成的cRNA中骨髓毒素特异性基因的表达水平，并比较未处理组的cRNA。 通过微阵列进行基因的表达水平。
-
Search Results
2
records
(took 0.046 seconds)
