公开(公告)号：KR100829987B1

公开(公告)日：2008-05-20

申请号：KR1020070109425

申请日：2007-10-30

Applicant: 대한민국 (식품의약품안전처장)

Inventor： 심영훈 ,  강신정 ,  강인호 ,  남용석 ,  조소연 ,  오세욱 ,  이병희 ,  현성예 ,  장승엽

IPC: C12Q1/68 ,  G01N33/53 ,  G01N33/15 ,  G01N33/02

CPC classification number: G01N33/15 ,  G01N33/02

Abstract: A primer set and a real time PCR detecting set for detecting deer are provided to identify the existence of horns of reindeer, which is mixed with the young antlers of the deer, thereby securing the quality and reliability of the young antlers of the deer. A primer set for detecting deer includes a primer pair, which is specific to the deer and has sequences of SEQ ID : NOs. 1 and 2. A real time PCR detection set for detecting deer comprises the primer pair and a probe, which is specific to the deer and has a sequence of SEQ ID : NO. 3. A method for distinguishing materials derived from deer comprises the steps of: (a) subjecting DNA extracted from a sample to real time PCR using the real time PCR detection set; and (b) identifying the real time PCR result obtained from the step(a) to determine whether the sample is derived from the deer or the sample is mixed with materials derived from reindeer. Further, the sample is a cell of an organism, a tissue of the organism, an organ of the organism and a part of body.

Abstract translation: 提供了一套用于检测鹿的引物组和实时PCR检测装置，用于识别与鹿的年轻鹿角混合的驯鹿角的存在，从而确保鹿的年轻鹿角的质量和可靠性。 用于检测鹿的引物组包括特异于鹿并具有SEQ ID NO：的序列的引物对。 用于检测鹿的实时PCR检测组包括引物对和探针，其特异于鹿并具有SEQ ID NO：1的序列。 3.鉴别衍生自鹿的物质的方法包括以下步骤：（a）使用实时PCR检测装置对从样品提取的DNA进行实时PCR; 和（b）鉴定从步骤（a）获得的实时PCR结果，以确定样品是从鹿获得的还是样品与来自驯鹿的材料混合。 此外，样品是生物体的细胞，生物体的组织，生物的器官和身体的一部分。