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公开(公告)号:KR100905414B1
公开(公告)日:2009-07-02
申请号:KR1020080060020
申请日:2008-06-25
Applicant: 대한민국 (식품의약품안전처장)
Abstract: A discrimination method of growing district of herbal medicine is provided to analysis the metabolite mixture without DNA analysis or standard material and discriminate the growing district of the herbal medicine. A method for discriminating the growing district of Scutellaria baicalensis Georgi. Comprises: a step of extracting metabolite from a plurality of groups of Scutellaria baicalensis Georgi. Sample; a step of analyzing the metabolite with 1H NMR(Nuclear magnetic resonance) spectroscopy; a step of converting the analyzed result of 1H NMR; a step of analyzing the converted value with multivariate analysis; a step of detecting the difference among the groups and establishing standard model; a step of discriminating the growing district; and a step of analyzing the metabolite from the Scutellaria baicalensis Georgi. and verifying the growing district.
Abstract translation: 提供了生长区草药的辨别方法,对DNA代谢物混合物进行分析,不用DNA分析或标准物质,从而区分中草药生长区。 一种鉴别黄芩生长区的方法。 包括:从多个黄芩黄芩提取代谢物的步骤。 样品; 用1 H NMR(核磁共振)光谱分析代谢物的步骤; 转化1H NMR的分析结果的步骤; 用多变量分析分析转换价值的一个步骤; 检测组间差异,建立标准模型的步骤; 区分增长区的一个步骤; 并分析了黄芩的代谢物。 并验证增长区。
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公开(公告)号:KR100829987B1
公开(公告)日:2008-05-20
申请号:KR1020070109425
申请日:2007-10-30
Applicant: 대한민국 (식품의약품안전처장)
Abstract: A primer set and a real time PCR detecting set for detecting deer are provided to identify the existence of horns of reindeer, which is mixed with the young antlers of the deer, thereby securing the quality and reliability of the young antlers of the deer. A primer set for detecting deer includes a primer pair, which is specific to the deer and has sequences of SEQ ID : NOs. 1 and 2. A real time PCR detection set for detecting deer comprises the primer pair and a probe, which is specific to the deer and has a sequence of SEQ ID : NO. 3. A method for distinguishing materials derived from deer comprises the steps of: (a) subjecting DNA extracted from a sample to real time PCR using the real time PCR detection set; and (b) identifying the real time PCR result obtained from the step(a) to determine whether the sample is derived from the deer or the sample is mixed with materials derived from reindeer. Further, the sample is a cell of an organism, a tissue of the organism, an organ of the organism and a part of body.
Abstract translation: 提供了一套用于检测鹿的引物组和实时PCR检测装置,用于识别与鹿的年轻鹿角混合的驯鹿角的存在,从而确保鹿的年轻鹿角的质量和可靠性。 用于检测鹿的引物组包括特异于鹿并具有SEQ ID NO:的序列的引物对。 用于检测鹿的实时PCR检测组包括引物对和探针,其特异于鹿并具有SEQ ID NO:1的序列。 3.鉴别衍生自鹿的物质的方法包括以下步骤:(a)使用实时PCR检测装置对从样品提取的DNA进行实时PCR; 和(b)鉴定从步骤(a)获得的实时PCR结果,以确定样品是从鹿获得的还是样品与来自驯鹿的材料混合。 此外,样品是生物体的细胞,生物体的组织,生物的器官和身体的一部分。
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