公开(公告)号：KR1020160037300A

公开(公告)日：2016-04-06

申请号：KR1020140129123

申请日：2014-09-26

Applicant: 동국대학교 산학협력단

Inventor： 김대영 ,  권구중 ,  조준형 ,  성정석

IPC: A01G31/00

Abstract: 본발명은폐목재칩및 폐지슬러지로이루어진양액재배용목재고형배지에관한것이다. 본발명은또한 a)목재와수피가혼합된폐목재칩을멸균처리하는단계; b) 폐지상지를파쇄하여폐지슬러지를제조하는단계; c) 상기폐목재칩및 폐지슬러리를혼합하는단계; d) 상기혼합물을성형틀에부은후 압착하는단계; 및e) 상기압착물을건조시키는단계;를포함하는양액재배용목재고형배지의제조방법에관한것이다.

Abstract translation: 本发明涉及由废木屑和废纸污泥制备的用于营养培养的垫型木材固体培养基。 另外，本发明涉及用于营养培养的垫型木材固体培养基的制备方法，其特征在于，该方法包括以下步骤：a）对包括树皮和木屑在内的废木屑进行消毒; b）粉碎废纸箱以生产废纸污泥; c）将废木屑和废纸污泥混合以产生混合物; d）将混合物倒入模具并压缩; 和e）干燥压缩产品。

公开(公告)号：KR1020100053456A

公开(公告)日：2010-05-20

申请号：KR1020090108115

申请日：2009-11-10

Applicant: 동국대학교 산학협력단 ,  한국생명공학연구원

Inventor： 조준형 ,  권석윤 ,  김영화 ,  김명수 ,  변지희

IPC: C12N15/82 ,  C12N15/63 ,  A01H1/00 ,  A01H4/00

Abstract: PURPOSE: A method for producing transgenic rice plants in which a selection marker is removed is provided to effectively obtain marker-free transgenic plants. CONSTITUTION: A method for producing transgenic rice plants in which a selection marker is removed comprises: a step of preparing an expression vector containing a positive-negative selection marker gene and foreign target gene; a step of introducing the expression vector to Agrobacterium; a step of co-culturing the expression vector-introduced Agrobacterium with a rice plant or tissue thereof; a step of treating transgenic rice plant with N-AcPt(N-acetyl-phosphinothricin); and a step of selecting transgenic rice plant in which a positive-negative selection marker gene is not contained. The positive selection marker gene is hpt gene or NPTII gene. The negative selection marker gene is argE gene. The expression vector is pSN-K(KCTC10538BP) or pSN-H(KCTC10539BP) vector.

Abstract translation: 目的：提供一种转基因水稻植物的制备方法，其中除去选择标记物以有效获得无标记转基因植物。 构成：其中除去选择标记的转基因水稻植物的制备方法包括：制备含有阳性 - 阴性选择标记基因和外来靶基因的表达载体的步骤; 将表达载体导入农杆菌的步骤; 用水稻植物或其组织共培养引入的表达载体的农杆菌的步骤; 用N-AcPt（N-乙酰基 - 膦丝菌素）处理转基因水稻植株的步骤; 以及选择不含有阳性 - 阴性选择标记基因的转基因水稻植物的步骤。 阳性选择标记基因是hpt基因或NPTII基因。 阴性选择标记基因是argE基因。 表达载体是pSN-K（KCTC10538BP）或pSN-H（KCTC10539BP）载体。