公开(公告)号：KR1020130139817A

公开(公告)日：2013-12-23

申请号：KR1020130148576

申请日：2013-12-02

Applicant: 서강대학교산학협력단

Inventor： 이정국 ,  김미선 ,  김의진 ,  오은경

IPC: C12P1/04 ,  C12N15/52 ,  C12N15/63

CPC classification number: C12P3/00 ,  C12N15/74

Abstract: The present invention relates to a method for screening oxygen-tolerant hydrogenase using a rhodobacter sphaeroides mutant which lacks a Quinone oxidase (QxtAB) gene and a cytochrome bc1 (FbcBC) gene in chromosome DNA, a method for preparing oxygen-tolerant hydrogenase, and a method for generating hydrogen by culturing recombinant rhodobacter sphaeroides which is transgened using recombinant vectors including gene for coding foreign hydrogen enzymes.

Abstract translation: 本发明涉及使用在染色体DNA中缺乏喹诺酮氧化酶（QxtAB）基因和细胞色素bc1（FbcBC））基因的罗汉斯杆菌球形体突变体筛选耐氧性氢化酶的方法，制备耐氧氢化酶的方法和 通过培养使用包含用于编码外源氢酶的基因的重组载体转基因的重组罗红假单胞菌来产生氢的方法。

公开(公告)号：KR1020130004947A

公开(公告)日：2013-01-15

申请号：KR1020110065649

申请日：2011-07-01

Applicant: 서강대학교산학협력단

Inventor： 이정국 ,  김미선 ,  김의진 ,  오은경

IPC: C12Q1/02 ,  C12N1/21 ,  C12N15/74 ,  C12N9/02

CPC classification number: C12Q1/26 ,  C12N9/0067 ,  C12N15/74 ,  C12P21/00

Abstract: PURPOSE: A method for screening oxygen tolerant hydrogenase using a Rhodobacter sphaeroides mutant is provided to screen the hydrogenase without measuring hydrogen generation amount. CONSTITUTION: A method for screening oxygen tolerant hydrogenase comprises: a step of preparing a Rhodobacter sphaeroides mutant in which quinine oxidase(QxtAB) gene and cytochrome bc1(FbcBC) gene are deleted; and a step of expressing foreign hydrogenase in the mutant strain and confirming the growth of the mutant strain. A method for producing hydrogen comprises a step of culturing recombinant Rhodobacter sphaeroides which is transformed using the recombinant vector under anaerobic and dark conditions. The recombinant vector contains a gene encoding the foreign hydrogenase. [Reference numerals] (AA) Successive removal of Quinol oxidase Qxt/Cytochromebcl; (BB) Introduction; (CC) Manufacturing a structure expressing cyanobacterium-derived hydrogenase; (DD) Acquiring recombinant; (EE) Separating suppressor mutant which survives under various oxygen partial pressures of 1%-20%; (FF) Acquring and analyzing HoxYH mutein

Abstract translation: 目的：提供使用罗红假单胞菌突变体筛选耐氧化氢化酶的方法，以筛选氢化酶而不测量氢生成量。 构成：用于筛选耐氧化氢化酶的方法包括：制备其中奎宁氧化酶（QxtAB）基因和细胞色素bc1（FbcBC））基因缺失的球形红细菌突变体的步骤; 以及在突变菌株中表达外源氢化酶并证实突变株的生长的步骤。 生产氢的方法包括培养在厌氧和黑暗条件下使用重组载体转化的重组球形红细菌球菌的步骤。 重组载体含有编码外源氢化酶的基因。 （标号）（AA）连续去除喹诺酮氧化酶Qxt / Cytochromebcl; （BB）介绍; （CC）制造表达蓝细菌衍生的氢化酶的结构; （DD）获得重组; （EE）分离抑制突变体，其在各种氧分压为1％-20％下存活; （FF）对HoxYH突变蛋白进行分析和分析