公开(公告)号：KR1020140106476A

公开(公告)日：2014-09-03

申请号：KR1020140093158

申请日：2014-07-23

Applicant: 서강대학교산학협력단

Inventor： 이현수 ,  박나영 ,  유지혜 ,  장소혜

IPC: C07K1/22

Abstract: Provided are a producing method of a protein mutant where some amino acid in sequences is substituted to non-natural amino acid containing residues of metal chelate, a protein mutant where some amino acid in sequences is substituted to non-natural amino acid containing residues of metal chelate, and a method for purifying protein using the protein mutant.

Abstract translation: 提供了一种蛋白质突变体的制备方法，其中序列中的一些氨基酸被替换为含有金属螯合物残基的非天然氨基酸，其中序列中的一些氨基酸被替换为含有金属残基的非天然氨基酸的蛋白质突变体 螯合物和使用蛋白质突变体纯化蛋白质的方法。

公开(公告)号：KR1020130117139A

公开(公告)日：2013-10-25

申请号：KR1020120039956

申请日：2012-04-17

Applicant: 서강대학교산학협력단

Inventor： 이현수 ,  박나영 ,  유지혜 ,  장소혜

IPC: C12N9/10 ,  C07K14/435 ,  C12P21/04 ,  C07K1/00

CPC classification number: C12P21/00 ,  C07K1/22 ,  C07K2319/23 ,  C12N9/1088 ,  C12Y205/01018

Abstract: PURPOSE: A mutant protein is provided to purify proteins with high purity by non-natural amino acids and to skip a step of removing a tag. CONSTITUTION: A method for preparing a mutant protein comprises the steps of: substituting a nucleic acid encoding a protein with a stop codon; and translating the substituted protein by non-natural amino acids containing metal chelating residues using tRNA and amino acyl tRNA synthetase. Proteins are purified using the mutant protein.

Abstract translation: 目的：提供突变蛋白以通过非天然氨基酸纯化蛋白质，并跳过去除标签的步骤。 构成：制备突变蛋白的方法包括以下步骤：用终止密码子取代编码蛋白质的核酸; 并使用tRNA和氨基酰基tRNA合成酶将含有金属螯合残基的非天然氨基酸翻译为取代蛋白。 使用突变蛋白纯化蛋白质。