公开(公告)号：KR1020140046302A

公开(公告)日：2014-04-18

申请号：KR1020120112609

申请日：2012-10-10

Applicant: 서강대학교산학협력단

Inventor： 이현수 ,  김동욱 ,  장소혜

IPC: C12Q1/37 ,  G01N33/58 ,  G01N33/52

CPC classification number: G01N33/58 ,  G01N33/68

Abstract: The present invention relates to a protein labeling method including unnatural amino acid, an analysis method of labeled protein, and a protein labeling composition. The protein labeling method comprises: a step of substituting amino acid contained in target protein using azide group-containing unnatural amino acid; and a step of labeling the target protein using a labeling reagent through a conjugation reaction between a cycloakynyl group contained in the labeling reagent and an azide group of the azide group-containing unnatural amino acid. [Reference numerals] (AA) Unnatural amino acid genetically introduced; (BB) Biochemical probe attached to ADIBO

Abstract translation: 本发明涉及包括非天然氨基酸的蛋白质标记方法，标记蛋白质的分析方法和蛋白质标记组合物。 蛋白质标记方法包括：使用含有叠氮基的非天然氨基酸取代靶蛋白中所含的氨基酸的步骤; 以及使用标记试剂通过包含在标记试剂中的环亚炔基和与含叠氮基的非天然氨基酸的叠氮基之间的共轭反应来标记靶蛋白的步骤。 （AA）遗传上引入的非天然氨基酸; （BB）生物化学探针附于ADIBO

公开(公告)号：KR101451229B1

公开(公告)日：2014-10-15

申请号：KR1020120112609

申请日：2012-10-10

Applicant: 서강대학교산학협력단

Inventor： 이현수 ,  김동욱 ,  장소혜

IPC: C12Q1/37 ,  G01N33/58 ,  G01N33/52

Abstract: 비천연 아미노산을 포함하는 단백질의 표지 방법, 표지된 단백질의 분석 방법, 및 단백질 표지용 조성물에 관한 것이다

公开(公告)号：KR1020140106476A

公开(公告)日：2014-09-03

申请号：KR1020140093158

申请日：2014-07-23

Applicant: 서강대학교산학협력단

Inventor： 이현수 ,  박나영 ,  유지혜 ,  장소혜

IPC: C07K1/22

Abstract: Provided are a producing method of a protein mutant where some amino acid in sequences is substituted to non-natural amino acid containing residues of metal chelate, a protein mutant where some amino acid in sequences is substituted to non-natural amino acid containing residues of metal chelate, and a method for purifying protein using the protein mutant.

Abstract translation: 提供了一种蛋白质突变体的制备方法，其中序列中的一些氨基酸被替换为含有金属螯合物残基的非天然氨基酸，其中序列中的一些氨基酸被替换为含有金属残基的非天然氨基酸的蛋白质突变体 螯合物和使用蛋白质突变体纯化蛋白质的方法。

公开(公告)号：KR1020130117139A

公开(公告)日：2013-10-25

申请号：KR1020120039956

申请日：2012-04-17

Applicant: 서강대학교산학협력단

Inventor： 이현수 ,  박나영 ,  유지혜 ,  장소혜

IPC: C12N9/10 ,  C07K14/435 ,  C12P21/04 ,  C07K1/00

CPC classification number: C12P21/00 ,  C07K1/22 ,  C07K2319/23 ,  C12N9/1088 ,  C12Y205/01018

Abstract: PURPOSE: A mutant protein is provided to purify proteins with high purity by non-natural amino acids and to skip a step of removing a tag. CONSTITUTION: A method for preparing a mutant protein comprises the steps of: substituting a nucleic acid encoding a protein with a stop codon; and translating the substituted protein by non-natural amino acids containing metal chelating residues using tRNA and amino acyl tRNA synthetase. Proteins are purified using the mutant protein.

Abstract translation: 目的：提供突变蛋白以通过非天然氨基酸纯化蛋白质，并跳过去除标签的步骤。 构成：制备突变蛋白的方法包括以下步骤：用终止密码子取代编码蛋白质的核酸; 并使用tRNA和氨基酰基tRNA合成酶将含有金属螯合残基的非天然氨基酸翻译为取代蛋白。 使用突变蛋白纯化蛋白质。