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公开(公告)号：KR1020130068092A
公开(公告)日：2013-06-25
申请号：KR1020110135488
申请日：2011-12-15
Applicant: 서강대학교산학협력단
IPC: A61K31/7088 , G01N33/15 , C12N15/113 , G01N33/68
CPC classification number: G01N33/15 , A61K31/7088 , C12N15/113 , G01N33/68 , G01N2500/04
Abstract: PURPOSE: A method for screening an inhibitor of radiation-induced vascular regeneration loss is provided to suppress reduction of vascular regeneration due to cellular senescence and functional damage to EPCs(endothelial progenitor cells). CONSTITUTION: A method for screening an inhibitor of radiation-induced vascular regeneration loss comprises: a step of contacting a sample with a cell containing p53 gene or protein; and a step of measuring the expression level p53 gene, or protein level or activation of p53 protein. The sample is determined as an inhibitor of radiation-induced vascular regeneration loss when the expression level, protein level, or activation is down-regulated. A composition for suppressing radiation-induced vascular regeneration loss contains a material as an active ingredient, which suppresses expression of p53 gene or reduces activation of p53 protein. A pharmaceutical composition for vascular regeneration contains the inhibitor as an active ingredient.
Abstract translation: 目的：提供一种筛选放射诱导的血管再生损失抑制剂的方法，以抑制由于EPCs（内皮祖细胞）的细胞衰老和功能损伤引起的血管再生的减少。 构成：用于筛选辐射诱导的血管再生损失抑制剂的方法包括：使样品与含有p53基因或蛋白质的细胞接触的步骤; 以及测量p53基因表达水平或蛋白水平或p53蛋白激活的步骤。 当表达水平，蛋白质水平或活化被下调时，样品被确定为辐射诱导的血管再生损失的抑制剂。 用于抑制辐射诱导的血管再生损失的组合物含有作为活性成分的材料，其抑制p53基因的表达或降低p53蛋白的活化。 用于血管再生的药物组合物含有作为活性成分的抑制剂。
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公开(公告)号：KR1020110042426A
公开(公告)日：2011-04-27
申请号：KR1020090099094
申请日：2009-10-19
Applicant: 서강대학교산학협력단
Abstract: PURPOSE: A method for differentiating human embryonic stem cells is provided to induce selective apoptosis of undifferentiated human embryonic stem cells. CONSTITUTION: A method for differentiating human embryonic stem cells comprises: a step of culturing embryoid derived from the human embryonic stem cells in a medium for differentiating to induce differentiation; a step of adding quercetin and culturing; and a step of inducing selective apoptosis of undifferentiated human embryonic stem cells to separate differentiated cells. The concentration of quercetin is 25-200 uM.
Abstract translation: 目的：提供一种区分人类胚胎干细胞的方法，以诱导未分化人胚胎干细胞的选择性凋亡。 构成：用于分化人类胚胎干细胞的方法包括：在用于分化以诱导分化的培养基中培养源自人胚胎干细胞的胚状体的步骤; 添加槲皮素和培养的步骤; 以及诱导未分化人胚胎干细胞选择性凋亡以分离分化细胞的步骤。 槲皮素浓度为25-200uM。
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公开(公告)号：KR101420752B1
公开(公告)日：2014-08-14
申请号：KR1020110135488
申请日：2011-12-15
Applicant: 서강대학교산학협력단
IPC: A61K31/7088 , G01N33/15 , C12N15/113 , G01N33/68
Abstract: 본 발명은 방사선-유도 EPC의 혈관재생성 소실 억제제를 스크리닝하는 방법에 관한 것이다. 보다 상세하게는, (a) p53 유전자 또는 단백질을 포함하는 세포에 분석하고자 하는 시료를 접촉시키는 단계; 및 (b) 상기 p53 유전자의 발현량, p53 단백질의 양 또는 p53 단백질의 활성을 측정하는 단계를 포함하는 방법으로, 상기 p53 유전자의 발현량, p53 단백질의 양 또는 p53 단백질의 활성이 감소-조절(down-regulation)되는 것으로 측정되는 경우에는 상기 시료는 방사선-유도성 혈관재생성 소실 억제제로 판정된다. 본 발명은 방사능 노출로 인해 세포 노화 및 EPCs의 기능 손상되어 혈관 재생이 감소되는 것을 억제할 수 있다.
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