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公开(公告)号：KR1020140052156A
公开(公告)日：2014-05-07
申请号：KR1020120116959
申请日：2012-10-19
Applicant: 서강대학교산학협력단
Abstract: The present invention relates to a transformed yeast for improving a fatty acid content and a method for producing same. Provided is a transformed yeast for producing transformed fatty acid through a recombinant vector comprising: (i) a nucleotide sequence that codes oxoacyl carrier protein synthetic enzyme II (fabF) that is derived from Saccharomyces cerevisiae; (ii) a nucleotide sequence that codes oxoacyl carrier protein reduced enzyme (fabG) that is derived from Streptococcus pyogenes; (iii) a nucleotide sequence that codes hydroxyl myristoyl carrier protein dehydrated enzyme (fabZ) that is derived from Streptococcus pyogenes; (iv) a nucleotide sequence that codes ynoyl carrier protein reduced enzyme II (fabK) that is derived from Streptococcus pyogenes; or (v) a nucleotide sequence that codes oleyl carrier protein dehydrated enzyme (EC 3.1.2.14) that is derived from Streptococcus pyogenes. In the present invention, by means of Saccharomyces cerevisiae, a fatty acid biosynthesis path extending step is over-expressed so that the content of fatty acid can be increased. Through the over-expression from glucose to a fatty acid biosynthesis path and changes in a metabolism flow by means of the insertion of a gene of a different kind, fatty acid biosynthesis can be effectively mass produced.
Abstract translation: 本发明涉及一种改善脂肪酸含量的转化酵母及其制备方法。 提供了通过重组载体产生转化的脂肪酸的转化酵母，其包含：（i）编码来源于酿酒酵母的氧代酰基载体蛋白质合成酶II（fabF）的核苷酸序列; （ii）编码源自化脓性链球菌的氧代酰基载体蛋白质还原酶（fabG）的核苷酸序列; （iii）编码衍生自化脓性链球菌的羟基肉豆蔻酰载体蛋白脱水酶（fabZ）的核苷酸序列; （iv）编码衍生自化脓性链球菌的甲酰基载体蛋白质还原酶II（fabK）的核苷酸序列; 或（v）编码来源于化脓性链球菌的油基载体蛋白脱水酶（EC 3.1.2.14）的核苷酸序列。 在本发明中，通过酿酒酵母，脂肪酸生物合成途径延伸步骤被过度表达，从而可以增加脂肪酸的含量。 通过从葡萄糖到脂肪酸生物合成途径的过度表达，通过插入不同种类的基因来改变代谢流，可以有效地大量生产脂肪酸生物合成。
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公开(公告)号：KR1020140026912A
公开(公告)日：2014-03-06
申请号：KR1020120092750
申请日：2012-08-24
Applicant: 서강대학교산학협력단
Abstract: The present invention relates to a method for preparing transformed Escherichia coli for expressing acyl-′acyl carrier protein′ thioesterase which includes the steps of: (a) obtaining a nucleotide sequence of first sequence in sequencing list by codon-optimization of a nucleotide sequence which codes Streptococcus pyogenes-derived acyl-′acyl carrier protein′ thioesterase in order to be optimized to be expressed in the Escherichia coli; (b) preparing a recombinant vector by inserting the codon-optimized nucleotide sequence of the acyl-′acyl carrier protein′ thioesterase; and (c) preparing recombinant Escherichia coli by transforming the recombinant vector into the Escherichia coli. The method can produce a large amount of fatty acids which will become the base of bioenergy production, are environmentally friendly, and have great financial power by increasing the production of saturated fatty acids of C12:0 and C14:0 and unsaturated fatty acids of C18:1 among fatty acids produced from Escherichia coli by 200-400% compared with the production by a wild type.
Abstract translation: 本发明涉及一种用于表达酰基 - 酰基载体蛋白'硫酯酶的转化大肠杆菌的制备方法，其包括以下步骤：（a）通过核苷酸序列的密码子优化获得测序列表中第一序列的核苷酸序列， 代谢化脓性链球菌衍生的酰基 - 酰基载体蛋白'硫酯酶，以优化在大肠杆菌中表达; （b）通过插入酰基 - 酰基 - 载体蛋白'硫酯酶的密码子优化的核苷酸序列来制备重组载体; 和（c）通过将重组载体转化到大肠杆菌中制备重组大肠杆菌。 该方法可以产生大量的脂肪酸，成为生物能源生产的基础，环保，通过增加C12：0和C14：0的饱和脂肪酸和C18不饱和脂肪酸的生产具有巨大的财力 ：大肠杆菌生产的脂肪酸中的1种与野生型相比，产量为200-400％。
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公开(公告)号：KR101879904B1
公开(公告)日：2018-07-19
申请号：KR1020140046079
申请日：2014-04-17
Applicant: 서강대학교산학협력단
Abstract: 본발명은지방산함량개선을위한형질전환효모및 그의제조방법에관한것으로, (ⅰ) 사카로미세스세레비시아() 유래의옥소아실운반단백질합성효소Ⅱ()를코딩하는뉴클레오타이드서열; (ⅱ) 스트렙토코커스피오지네스() 유래의옥소아실운반단백질환원효소()를코딩하는뉴클레오타이드서열; (ⅲ) 스트렙토코커스피오지네스유래의하이드록시미리스토일운반단백질탈수효소()를코딩하는뉴클레오타이드서열; (ⅳ)스트렙토코커스피오지네스유래의이노일운반단백질환원효소Ⅱ()를코딩하는뉴클레오타이드서열; 또는 (ⅴ) 스트렙토코커스피오지네스유래의올레일운반단백질가수분해효소(EC 3.1.2.14)를코딩하는뉴클레오타이드서열을포함하는재조합벡터로형질전환된지방산생산용형질전환효모를제공한다. 본발명은에의하여지방산생합성경로의신장단계를과발현하여지방산자체의함량을증진할수 있어, 글루코스로부터지방산생합성경로에의과발현과다른종의유전자삽입에의한대사흐름변경을통해지방산생합성을대량생산해내는데효과적이다.
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Patent Agency Ranking
公开(公告)号：KR101469689B1
公开(公告)日：2014-12-08
申请号：KR1020120116959
申请日：2012-10-19
Applicant: 서강대학교산학협력단
Abstract: 본발명은지방산함량개선을위한형질전환효모및 그의제조방법에관한것으로, (ⅰ) 사카로미세스세레비시아() 유래의옥소아실운반단백질합성효소Ⅱ()를코딩하는뉴클레오타이드서열; (ⅱ) 스트렙토코커스피오지네스() 유래의옥소아실운반단백질환원효소()를코딩하는뉴클레오타이드서열; (ⅲ) 스트렙토코커스피오지네스유래의하이드록시미리스토일운반단백질탈수효소()를코딩하는뉴클레오타이드서열; (ⅳ)스트렙토코커스피오지네스유래의이노일운반단백질환원효소Ⅱ()를코딩하는뉴클레오타이드서열; 또는 (ⅴ) 스트렙토코커스피오지네스유래의올레일운반단백질가수분해효소(EC 3.1.2.14)를코딩하는뉴클레오타이드서열을포함하는재조합벡터로형질전환된지방산생산용형질전환효모를제공한다. 본발명은에의하여지방산생합성경로의신장단계를과발현하여지방산자체의함량을증진할수 있어, 글루코스로부터지방산생합성경로에의과발현과다른종의유전자삽입에의한대사흐름변경을통해지방산생합성을대량생산해내는데효과적이다.
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公开(公告)号：KR101568401B1
公开(公告)日：2015-11-13
申请号：KR1020130110540
申请日：2013-09-13
Applicant: 서강대학교산학협력단
Abstract: 본발명은지방산생산용형질전환효모에관한것으로, 스트렙토코커스피오지네스() 유래의 (ⅰ) 옥소아실운반단백질환원효소()를코딩하는뉴클레오타이드서열; (ⅱ) 하이드록시미리스토일운반단백질탈수효소()를코딩하는뉴클레오타이드서열; (ⅲ) 이노일운반단백질환원효소Ⅱ()를코딩하는뉴클레오타이드서열; 및 (ⅳ) 올레일운반단백질가수분해효소()를코딩하는뉴클레오타이드서열로구성된군으로부터선택되는하나이상의뉴클레오타이드서열을포함하는재조합벡터로형질전환된지방산생산용형질전환효모이다. 본발명에따르면, 본발명의지방산생산용형질전환효모는스트렙토코커스피오지네스유래의지방산관련효소를동시에발현하고, 우수한지방산생산능을갖는다.
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公开(公告)号：KR1020150031375A
公开(公告)日：2015-03-24
申请号：KR1020130110540
申请日：2013-09-13
Applicant: 서강대학교산학협력단
Abstract: 본 발명은 지방산 생산용 형질전환 효모에 관한 것으로, 스트렙토코커스 피오지네스(
Streptococcus pyogenes ) 유래의 (ⅰ) 옥소아실 운반단백질 환원효소(
fabG )를 코딩하는 뉴클레오타이드 서열; (ⅱ) 하이드록시미리스토일 운반단백질 탈수효소(
fabZ )를 코딩하는 뉴클레오타이드 서열; (ⅲ) 이노일 운반단백질 환원효소 Ⅱ(
fabK )를 코딩하는 뉴클레오타이드 서열; 및 (ⅳ) 올레일 운반단백질 가수분해효소(
EC
3.1.2.14 )를 코딩하는 뉴클레오타이드 서열로 구성된 군으로부터 선택되는 하나 이상의 뉴클레오타이드 서열을 포함하는 재조합 벡터로 형질전환된 지방산 생산용 형질전환 효모이다. 본 발명에 따르면, 본 발명의 지방산 생산용 형질전환 효모는 스트렙토코커스 피오지네스 유래의 지방산 관련 효소를 동시에 발현하고, 우수한 지방산 생산능을 갖는다.Abstract translation: 本发明涉及用于生产脂肪酸的转基因酵母，其通过使用包含至少一种选自以下的核苷酸序列的重组载体转化：（i）编码氧代酰基递送蛋白还原酶（fabG）的核苷酸序列， 衍生自化脓性链球菌; （ii）编码羟基肉豆蔻酰蛋白递送蛋白脱水酶（fabZ）的核苷酸序列; （iii）编码递送蛋白质还原酶II（fabK）的核苷酸序列; 和（iv）编码油基递送蛋白水解酶的核苷酸序列（EC3.1.2.14）。 根据本发明，用于生产脂肪酸的转基因酵母同时表达与来自化脓性链球菌的脂肪酸相关的酶，并具有突出的产生脂肪酸的能力。
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公开(公告)号：KR1020140071293A
公开(公告)日：2014-06-11
申请号：KR1020140046079
申请日：2014-04-17
Applicant: 서강대학교산학협력단
Abstract: The present invention relates to transformed yeast for improving the content of fatty acids and a preparation method thereof and provides the transformed yeast for producing fatty acids which is transformed with a recombination vector comprising; (i) a nucleotide sequence which codes an oxoacyl carrier protein synthetase II (fabF) derived from Saccharomyces cerevisiae; (ii) a nucleotide sequence which codes oxoacyl carrier protein reductase (fabG) derived from Streptococcus pyogenes; (iii) a nucleotide sequence which codes a hydroxyl myristoyl carrier protein dehydratase (fabZ) derived from Streptococci pyogenes; (iv) a nucleotide sequence which codes ynoyl carrier protein reductase II (fabI) derived from Streptococcus pyogenes; or (v) a nucleotide sequence which codes oleyl carrier protein hydrolase (EC 3.1.2.14) derived from Streptococcus pyogenes. The present invention increases the content of the fatty acids itself by over-expressing an extension step of a fatty acid biosynthesis path by Saccharomyces cerevisiae and mass-produces fatty acid biosynthesis through a metabolism flow change due to the over-expression of the acid biosynthesis path from glucose and the insertion of other species.
Abstract translation: 本发明涉及用于改善脂肪酸含量的转化酵母及其制备方法，并提供用于制备用重组载体转化的脂肪酸的转化酵母，其包含： （i）编码源自酿酒酵母的氧代酰基载体蛋白质合成酶II（fabF）的核苷酸序列; （ii）编码源自化脓性链球菌的氧代酰基载体蛋白还原酶（fabG）的核苷酸序列; （iii）编码源自链球菌化脓链球菌的羟基肉豆蔻酰载体蛋白脱水酶（fabZ）的核苷酸序列; （iv）编码源自化脓性链球菌的月桂酰载体蛋白还原酶II（fabI）的核苷酸序列; 或（v）编码源自化脓链球菌的油基载体蛋白水解酶（EC 3.1.2.14）的核苷酸序列。 本发明通过过表达酿酒酵母的脂肪酸生物合成途径的延伸步骤而增加脂肪酸本身的含量，并且由于酸生物合成途径的过度表达而通过代谢流动变化大规模生产脂肪酸生物合成 从葡萄糖和其他物种的插入。
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