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公开(公告)号：KR1020140052156A
公开(公告)日：2014-05-07
申请号：KR1020120116959
申请日：2012-10-19
Applicant: 서강대학교산학협력단
Abstract: The present invention relates to a transformed yeast for improving a fatty acid content and a method for producing same. Provided is a transformed yeast for producing transformed fatty acid through a recombinant vector comprising: (i) a nucleotide sequence that codes oxoacyl carrier protein synthetic enzyme II (fabF) that is derived from Saccharomyces cerevisiae; (ii) a nucleotide sequence that codes oxoacyl carrier protein reduced enzyme (fabG) that is derived from Streptococcus pyogenes; (iii) a nucleotide sequence that codes hydroxyl myristoyl carrier protein dehydrated enzyme (fabZ) that is derived from Streptococcus pyogenes; (iv) a nucleotide sequence that codes ynoyl carrier protein reduced enzyme II (fabK) that is derived from Streptococcus pyogenes; or (v) a nucleotide sequence that codes oleyl carrier protein dehydrated enzyme (EC 3.1.2.14) that is derived from Streptococcus pyogenes. In the present invention, by means of Saccharomyces cerevisiae, a fatty acid biosynthesis path extending step is over-expressed so that the content of fatty acid can be increased. Through the over-expression from glucose to a fatty acid biosynthesis path and changes in a metabolism flow by means of the insertion of a gene of a different kind, fatty acid biosynthesis can be effectively mass produced.
Abstract translation: 本发明涉及一种改善脂肪酸含量的转化酵母及其制备方法。 提供了通过重组载体产生转化的脂肪酸的转化酵母，其包含：（i）编码来源于酿酒酵母的氧代酰基载体蛋白质合成酶II（fabF）的核苷酸序列; （ii）编码源自化脓性链球菌的氧代酰基载体蛋白质还原酶（fabG）的核苷酸序列; （iii）编码衍生自化脓性链球菌的羟基肉豆蔻酰载体蛋白脱水酶（fabZ）的核苷酸序列; （iv）编码衍生自化脓性链球菌的甲酰基载体蛋白质还原酶II（fabK）的核苷酸序列; 或（v）编码来源于化脓性链球菌的油基载体蛋白脱水酶（EC 3.1.2.14）的核苷酸序列。 在本发明中，通过酿酒酵母，脂肪酸生物合成途径延伸步骤被过度表达，从而可以增加脂肪酸的含量。 通过从葡萄糖到脂肪酸生物合成途径的过度表达，通过插入不同种类的基因来改变代谢流，可以有效地大量生产脂肪酸生物合成。
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Patent Agency Ranking
公开(公告)号：KR101442682B1
公开(公告)日：2014-09-19
申请号：KR1020120038484
申请日：2012-04-13
Applicant: 서강대학교산학협력단
Abstract: 본 발명은 (a) 미생물의 바이오매스를 냉장 메탄올(cold methanol)에 현탁한 다음 동결시키는 단계; (b) 상기 동결된 바이오매스를 해동시키는 단계; (c) 상기 단계 (a) 및 (b)를 반복하여 상기 바이오매스로부터 누출된 대사물의 현탁액을 얻는 단계; 및 (d) 상기 대사물의 현탁액에 대하여 매스 스펙트로미트리(mass spectrometry) 분석을 실시하여 상기 미생물의 세포 내 대사물을 동정하는 단계를 포함하는 미생물의 세포 내 대사 프로파일(intracellular metabolic profile)을 얻는 방법을 제공한다. 본 발명의 방법에 따르면, 본 발명은 고생산 및 고수율의 대사 프로파일을 제공할 수 있다.
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公开(公告)号：KR1020140071293A
公开(公告)日：2014-06-11
申请号：KR1020140046079
申请日：2014-04-17
Applicant: 서강대학교산학협력단
Abstract: The present invention relates to transformed yeast for improving the content of fatty acids and a preparation method thereof and provides the transformed yeast for producing fatty acids which is transformed with a recombination vector comprising; (i) a nucleotide sequence which codes an oxoacyl carrier protein synthetase II (fabF) derived from Saccharomyces cerevisiae; (ii) a nucleotide sequence which codes oxoacyl carrier protein reductase (fabG) derived from Streptococcus pyogenes; (iii) a nucleotide sequence which codes a hydroxyl myristoyl carrier protein dehydratase (fabZ) derived from Streptococci pyogenes; (iv) a nucleotide sequence which codes ynoyl carrier protein reductase II (fabI) derived from Streptococcus pyogenes; or (v) a nucleotide sequence which codes oleyl carrier protein hydrolase (EC 3.1.2.14) derived from Streptococcus pyogenes. The present invention increases the content of the fatty acids itself by over-expressing an extension step of a fatty acid biosynthesis path by Saccharomyces cerevisiae and mass-produces fatty acid biosynthesis through a metabolism flow change due to the over-expression of the acid biosynthesis path from glucose and the insertion of other species.
Abstract translation: 本发明涉及用于改善脂肪酸含量的转化酵母及其制备方法，并提供用于制备用重组载体转化的脂肪酸的转化酵母，其包含： （i）编码源自酿酒酵母的氧代酰基载体蛋白质合成酶II（fabF）的核苷酸序列; （ii）编码源自化脓性链球菌的氧代酰基载体蛋白还原酶（fabG）的核苷酸序列; （iii）编码源自链球菌化脓链球菌的羟基肉豆蔻酰载体蛋白脱水酶（fabZ）的核苷酸序列; （iv）编码源自化脓性链球菌的月桂酰载体蛋白还原酶II（fabI）的核苷酸序列; 或（v）编码源自化脓链球菌的油基载体蛋白水解酶（EC 3.1.2.14）的核苷酸序列。 本发明通过过表达酿酒酵母的脂肪酸生物合成途径的延伸步骤而增加脂肪酸本身的含量，并且由于酸生物合成途径的过度表达而通过代谢流动变化大规模生产脂肪酸生物合成 从葡萄糖和其他物种的插入。
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公开(公告)号：KR1020130115815A
公开(公告)日：2013-10-22
申请号：KR1020120038484
申请日：2012-04-13
Applicant: 서강대학교산학협력단
Abstract: PURPOSE: An extraction and analysis method of microorganisms for acquiring an intracellular metabolic profile is provided to obtain a metabolic profile of high productivity and high yield and to construct an optimal method for preparing intracellular metabolites. CONSTITUTION: A method for acquiring an intracellular metabolic profile in microorganisms comprises the steps of: suspending microorganism biomass in cold methanol and freezing the biomass; thawing the biomass and repeating the steps; collecting a suspension of the metabolites released from the biomass; and performing mass spectrometry of the suspension and identifying the intracellular metabolites.
Abstract translation: 目的：提供获取细胞内代谢特征的微生物提取和分析方法，以获得高生产率和高产量的代谢特征，并构建细胞内代谢产物的最佳方法。 构成：在微生物中获得细胞内代谢分布的方法包括以下步骤：将微生物生物质悬浮在冷甲醇中并冷冻生物质; 解冻生物质并重复步骤; 收集从生物质释放的代谢物的悬浮液; 并进行悬浮液的质谱分析和鉴定细胞内代谢物。
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公开(公告)号：KR101879904B1
公开(公告)日：2018-07-19
申请号：KR1020140046079
申请日：2014-04-17
Applicant: 서강대학교산학협력단
Abstract: 본발명은지방산함량개선을위한형질전환효모및 그의제조방법에관한것으로, (ⅰ) 사카로미세스세레비시아() 유래의옥소아실운반단백질합성효소Ⅱ()를코딩하는뉴클레오타이드서열; (ⅱ) 스트렙토코커스피오지네스() 유래의옥소아실운반단백질환원효소()를코딩하는뉴클레오타이드서열; (ⅲ) 스트렙토코커스피오지네스유래의하이드록시미리스토일운반단백질탈수효소()를코딩하는뉴클레오타이드서열; (ⅳ)스트렙토코커스피오지네스유래의이노일운반단백질환원효소Ⅱ()를코딩하는뉴클레오타이드서열; 또는 (ⅴ) 스트렙토코커스피오지네스유래의올레일운반단백질가수분해효소(EC 3.1.2.14)를코딩하는뉴클레오타이드서열을포함하는재조합벡터로형질전환된지방산생산용형질전환효모를제공한다. 본발명은에의하여지방산생합성경로의신장단계를과발현하여지방산자체의함량을증진할수 있어, 글루코스로부터지방산생합성경로에의과발현과다른종의유전자삽입에의한대사흐름변경을통해지방산생합성을대량생산해내는데효과적이다.
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公开(公告)号：KR101469689B1
公开(公告)日：2014-12-08
申请号：KR1020120116959
申请日：2012-10-19
Applicant: 서강대학교산학협력단
Abstract: 본발명은지방산함량개선을위한형질전환효모및 그의제조방법에관한것으로, (ⅰ) 사카로미세스세레비시아() 유래의옥소아실운반단백질합성효소Ⅱ()를코딩하는뉴클레오타이드서열; (ⅱ) 스트렙토코커스피오지네스() 유래의옥소아실운반단백질환원효소()를코딩하는뉴클레오타이드서열; (ⅲ) 스트렙토코커스피오지네스유래의하이드록시미리스토일운반단백질탈수효소()를코딩하는뉴클레오타이드서열; (ⅳ)스트렙토코커스피오지네스유래의이노일운반단백질환원효소Ⅱ()를코딩하는뉴클레오타이드서열; 또는 (ⅴ) 스트렙토코커스피오지네스유래의올레일운반단백질가수분해효소(EC 3.1.2.14)를코딩하는뉴클레오타이드서열을포함하는재조합벡터로형질전환된지방산생산용형질전환효모를제공한다. 본발명은에의하여지방산생합성경로의신장단계를과발현하여지방산자체의함량을증진할수 있어, 글루코스로부터지방산생합성경로에의과발현과다른종의유전자삽입에의한대사흐름변경을통해지방산생합성을대량생산해내는데효과적이다.
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