公开(公告)号：KR1020110092892A

公开(公告)日：2011-08-18

申请号：KR1020100012578

申请日：2010-02-10

Applicant: 서울대학교산학협력단

Inventor： 김원 ,  김성민 ,  강승현 ,  황승용 ,  김지훈 ,  구혜영 ,  최준길

IPC: C12Q1/68 ,  C12N15/11

CPC classification number: C12Q1/6827 ,  C12Q1/6837 ,  C12Q1/6844 ,  C12Q1/6888

Abstract: PURPOSE: A method for analyzing genotype of Korean fresh water fishes is provided to accurately analyze genotype and to shorten analysis time. CONSTITUTION: A method for determining classification of inter species of Korean fresh water fishes comprises: a step of isolating DNA from blood, cells, or tissue of Korean fresh water fishes; a step of performing PCR of the DNA to prepare a PCR product; a step of binding a polynucleotide probe which is identical or complementary with a DNA sequence among sequence numbers 3-28 with the PCR product; and a step of determining classification system.

Abstract translation: 目的：分析韩国淡水鱼类基因型的分析方法，准确分析基因型，缩短分析时间。 构成：确定韩国淡水鱼种间分类的方法，包括：从韩国淡水鱼血液，细胞或组织中分离DNA的步骤; 进行DNA的PCR以制备PCR产物的步骤; 将与序列号3-28中的DNA序列相同或互补的多核苷酸探针与PCR产物结合的步骤; 并确定分类系统的一个步骤。

公开(公告)号：KR101140022B1

公开(公告)日：2012-05-02

申请号：KR1020100012578

申请日：2010-02-10

Applicant: 서울대학교산학협력단

Inventor： 김원 ,  김성민 ,  강승현 ,  황승용 ,  김지훈 ,  구혜영 ,  최준길

IPC: C12Q1/68 ,  C12N15/11

Abstract: PURPOSE: A method for analyzing genotype of Korean fresh water fishes is provided to accurately analyze genotype and to shorten analysis time. CONSTITUTION: A method for determining classification of inter species of Korean fresh water fishes comprises: a step of isolating DNA from blood, cells, or tissue of Korean fresh water fishes; a step of performing PCR of the DNA to prepare a PCR product; a step of binding a polynucleotide probe which is identical or complementary with a DNA sequence among sequence numbers 3-28 with the PCR product; and a step of determining classification system.

公开(公告)号：KR1020100128118A

公开(公告)日：2010-12-07

申请号：KR1020090046573

申请日：2009-05-27

Applicant: 서울대학교산학협력단

Inventor： 김원 ,  김성민 ,  황승용 ,  김예림 ,  구혜영 ,  최준길 ,  어해석 ,  송교홍

IPC: C12Q1/68 ,  C12N15/11

CPC classification number: C12Q1/6888 ,  C12N15/11 ,  C12Q1/6827 ,  C12Q1/686

Abstract: PURPOSE: A method for determining classification system of inter species of fish living in fresh water is provided to simply analyze genotype of pre-larva, post-larva, young or juveniles, unmature and adult fish. CONSTITUTION: A method for determining classification of inter species of fish living in fresh water comprises: a step of isolating DNA from blood, cells or tissue of fish living in fresh water; a step of performing PCR; a step of binding the PCR product with a polynucleotide probe having identical or complementary sequence with DNA sequence selected from sequence numbers 3-18; and a step of determining classification system.

Abstract translation: 目的：提供一种确定生活在淡水中的鱼类种间分类系统的方法，简单分析幼虫，幼虫，幼鱼或幼鱼，未成熟鱼和成年鱼的基因型。 构成：一种确定生活在淡水中的鱼类种间分类方法，包括：从生活在淡水中的鱼血液，细胞或组织中分离DNA; 进行PCR的步骤; 将PCR产物与具有选自序列号3-18的DNA序列的具有相同或互补序列的多核苷酸探针结合的步骤; 并确定分类系统的一个步骤。