公开(公告)号：WO2014157820A1

公开(公告)日：2014-10-02

申请号：PCT/KR2014/000657

申请日：2014-01-23

Applicant: 서울대학교산학협력단

Inventor： 제연호 ,  최재영 ,  김송은 ,  김재수

IPC: C12N15/32 ,  C07K14/325 ,  A01N63/02

CPC classification number: C07K14/325

Abstract: 본 발명은 배추좀나방, 파밤나방, 옥수수조명나방 등 나비목 해충에 대한 살충활성이 향상된 신규의 바실러스 트린지엔시스 내독소단백질 유전자 및 그 제조방법에 관한 것으로, 해충 저항성 형질전환 작물의 제작을 위하여 다중부위 특정 돌연변이유발(muti site-directed mutagenesis)를 통해 나비목 해충에 살충활성을 가지는 크라이1-유형( cry1 -type) 내독소단백질의 기주범위와 관련된 아미노산 잔기 서열이 치환된 신규 재조합 내독소단백질 유전자 및 그 제조 방법을 제공한다.

Abstract translation: 本发明涉及一种具有增强杀虫活性的苏云金芽孢杆菌内毒素蛋白的新型基因，例如小菜蛾（Plutella xylostella），草地夜蛾（Spodoptera exigua）和鸵鸟（Ostrinia furnacalis）及其制备方法。 本发明提供了一种重组内毒素蛋白质的新基因，其中通过多位点定向诱变取代与具有杀虫活性的宿主范围的cry1型内毒素蛋白质相关的氨基酸残基序列，以产生昆虫 - 耐转基因作物及其制备方法。

公开(公告)号：KR1020100052254A

公开(公告)日：2010-05-19

申请号：KR1020080111192

申请日：2008-11-10

Applicant: 충북대학교 산학협력단 ,  동아대학교 산학협력단 ,  서울대학교산학협력단 ,  주식회사 중앙백신연구소 ,  주식회사 팜한농

Inventor： 우수동 ,  구현나 ,  진병래 ,  이광식 ,  제연호 ,  노종열 ,  최재영 ,  윤인중 ,  유성식 ,  김재수 ,  김영인

IPC: C12N15/34 ,  C12N15/09

Abstract: PURPOSE: A gene sequence encoding glycoprotein gB, gC, and gD of swine Aujeszky's disease virus(ADV) is provided to develop virus vaccine and diagnosis reagent and apparatus and to prevent miscarriage of pregnant pig. CONSTITUTION: A DNA fragment encoding glycoprotein gB of swine Aujeszky's disease virus(ADV) NYJ(KCTC 11132BP) has a base sequence of sequence number 1. A DNA fragment encoding glycoprotein gC of swine Aujeszky's disease virus(ADV) NYJ(KCTC 11132BP) has a sequence of sequence number 2. A DNA fragment encoding glycoprotein gD of swine Aujeszky's disease virus(ADV) NYJ(KCTC 11132BP) has a sequence of sequence number 3. A polypeptide encoded by the DNA fragment has amino acid sequences of sequence number 4(gB), 5(gC), and 6(gD).

Abstract translation: 目的：提供编码猪Aujeszky病病毒（ADV）的糖蛋白gB，gC和gD的基因序列，开发病毒疫苗和诊断试剂和仪器，防止怀孕猪流产。 构成：编码猪Aujeszky病病毒（ADV）NYJ（KCTC 11132BP）的糖蛋白gB的DNA片段具有序列号1的碱基序列。编码猪Aujeszky病病毒（ADV）NYJ（KCTC 11132BP）的糖蛋白gC的DNA片段具有 序列号为2的序列。编码猪Aujeszky病病毒（ADV）NYJ（KCTC 11132BP）的糖蛋白gD的DNA片段具有序列号3的序列。由该DNA片段编码的多肽具有序列号4的氨基酸序列（ gB），5（gC）和6（gD）。

公开(公告)号：KR1020140117257A

公开(公告)日：2014-10-07

申请号：KR1020140004841

申请日：2014-01-15

Applicant: 서울대학교산학협력단

Inventor： 제연호 ,  최재영 ,  김송은 ,  김재수

IPC: C12N15/32 ,  C12N15/63 ,  C12N15/70 ,  C12Q1/68

CPC classification number: C07K14/325 ,  A01N63/02 ,  C12N15/8286 ,  Y02A40/162 ,  C12N15/11 ,  C12N15/63 ,  C12N15/70

Abstract: The present invention relates to a novel bacillus thuringiensis endotoxic protein gene with improved insecticidal activities against lepidopteran insects and to a method for producing the same. More specifically, the present invention relates to a method for producing a bacillus thuringiensis endotoxic gene with strong insecticidal activities against lepidopteran insects such as Mamestra brassicae, Spodoptera litura, and Spodoptera exigua. The purpose of the present invention is achieved through the steps of: confirming a base sequence and a tertiary structure of a mod-cry1AC gene; producing a PIM-mod-cry1AC vector using the mod-cry1AC gene obtained from the previous step; producing a mutation-inducing primer using the mod-cry1AC as a mold; performing a polymerase chain reaction (PCR) using the mutation-inducing primer obtained from the previous step so as to produce novel BT endotoxic protein gene to be transformed in colon bacilli and confirming mutations; and confirming a base sequence and a tertiary structure of the novel BT endotoxic protein gene obtained from the previous step. Accordingly, in the present invention, agricultural products, which are not damaged by larvae of various lepidopteran insects, can be produced using a microorganism formulation including novel recombinant endotoxic protein gene Mut-N16.

Abstract translation: 本发明涉及一种具有改善的对鳞翅目昆虫的杀虫活性的新型苏云金芽孢杆菌内毒素蛋白基因及其制备方法。 更具体地说，本发明涉及一种对鳞翅目昆虫（如Mamestra brassicae），斜纹夜蛾（Spodoptera litura）和斜纹夜蛾（Spodoptera exigua）具有强杀虫活性的苏云金芽孢杆菌内毒素基因的生产方法。 通过以下步骤实现本发明的目的：确认mod-cry1AC基因的碱基序列和三级结构; 使用从前一步获得的mod-cry1AC基因产生PIM-mod-cry1AC载体; 使用mod-cry1AC作为模具产生突变诱导引物; 使用从前一步骤获得的突变诱导引物进行聚合酶链反应（PCR），以产生要在大肠杆菌中转化的新的BT内毒素蛋白基因并确认突变; 并确认从前一步骤获得的新型BT内毒素蛋白质基因的碱基序列和三级结构。 因此，在本发明中，可以使用包含新型重组内毒素蛋白质基因Mut-N16的微生物制剂来制造未被各种鳞翅目昆虫的幼虫损伤的农产品。

公开(公告)号：KR101555124B1

公开(公告)日：2015-09-22

申请号：KR1020140004841

申请日：2014-01-15

Applicant: 서울대학교산학협력단

Inventor： 제연호 ,  최재영 ,  김송은 ,  김재수

IPC: C12N15/32 ,  C12N15/63 ,  C12N15/70 ,  C12Q1/68

CPC classification number: C07K14/325 ,  A01N63/02 ,  C12N15/8286 ,  Y02A40/162

Abstract: 본발명은나비목해충에대한살충활성이향상된신규의바실러스트린지엔시스내독소단백질유전자및 그제조방법에관한것으로, 더욱상세하게는도둑나방, 담배거세미나방, 파밤나방등 나비목해충에강력한살충활성을갖고있는바실러스트린지엔시스내독소단백질유전자의제조방법에관한것이며본 발명의상기목적은모드-크라이1에이씨유전자의염기서열및 3차구조를확인하는단계와; 상기단계에서얻은모드-크라이1에이씨유전자를이용하여피아이엠-모드-크라이1에이씨벡터를제조하는단계와; 상기모드-크라이1에이씨를주형으로하여돌연변이유발프라이머를제조하는단계와; 상기단계에서얻은돌연변이유발프라이머를이용하여유전자증폭반응(Polymerase Chain Reaction, PCR)을수행하여신규비티내독소단백질유전자를제조한후 대장균에형질전환하여돌연변이여부를확인하는단계와; 상기단계에서얻은신규비티내독소단백질유전자의염기서열및 3차구조를확인하는단계를통하여달성하였으며따라서본 발명은신규한재조합내독소단백질유전자뮤트-엔16(Mut-N16)을포함하는미생물제제를이용하여다양한나비목해충의유충에의한피해가전혀없는농산물을생산할수 있는뛰어난효과가있다.

公开(公告)号：KR1020100049998A

公开(公告)日：2010-05-13

申请号：KR1020080109071

申请日：2008-11-04

Applicant: 충북대학교 산학협력단 ,  동아대학교 산학협력단 ,  서울대학교산학협력단 ,  주식회사 중앙백신연구소 ,  주식회사 팜한농

Inventor： 우수동 ,  구현나 ,  진병래 ,  이광식 ,  제연호 ,  노종열 ,  최재영 ,  윤인중 ,  유성식 ,  김재수 ,  김영인

IPC: C12N15/41 ,  C12N15/09 ,  C07K14/005

Abstract: PURPOSE: A novel porcine reproductive and respiratory syndrome virus(PRRSV) is provided to research of replication, transcription, and translation mechanism of PRRSV and develop diagnosis reagent and apparatus. CONSTITUTION: A DNA fragment encoding structure protein of porcine reproductive respiratory syndrome virus(PRRSV) is denoted by sequence number 1. The DNA fragment is denoted by sequence number 2, 3, 4, 5, 6, or 7. A vaccine composition for introducing antibody to PRRSV contains a polypeptide encoding with DNA fragment sequence.

Abstract translation: 目的：提供一种新型猪繁殖与呼吸综合征病毒（PRRSV），用于研究PRRSV的复制，转录和翻译机制，开发诊断试剂和仪器。 构成：编码猪繁殖呼吸综合征病毒（PRRSV）结构蛋白的DNA片段由序列号1表示.DNA片段由序列号2,3,4,5,6或7表示。用于引入的疫苗组合物 对PRRSV的抗体含有编码DNA片段序列的多肽。